E-CBIL-26 - Transcription profiling of mouse brown preadipocytes from wild type and IRS knock-out cell lines
Released on 6 April 2007, last updated on 31 July 2014
Brown preadipocytes were grown to confluence and synchronized by overnight serum starvation. Four independent RNA samples were analyzed from each IRS KO cell line and three independent clones of WT cells were separately analyzed as controls. Splitting of the samples resulted in a total 28 microarrays. 15 mg of adjusted cRNA were hybridized to Affymetrix U74A-v2 arrays.
transcription profiling by array, genetic modification
Prediction of preadipocyte differentiation by gene expression reveals role of insulin receptor substrates and necdin. Tseng Yu-Hua, Butte Atul J, Kokkotou Efi, Yechoor Vijay K, Taniguchi Cullen M, Kriauciunas Kristina M, Cypess Aaron M, Niinobe Michio, Yoshikawa Kazuaki, Patti Mary Elizabeth, Kahn C Ronald. , PMID:15895078