E-CBIL-20 - Transcription profiling of human pancreatic islets from normal and Type 2 diabetic subjects
Released on 28 July 2009, last updated on 2 June 2014
Human pancreatic islets isolated from 7 people with normal glucose tolerance, and 5 people with type 2 diabetes. All 12 people were organ donors after either cerebrovascular accident or intracerebral haemorrhage. Normals were required to maintain glucose at least 6.1mM in intensive care. Diabetic subjects were all at least 10 years from diagnosis and not insulin-requiring. For every subject, RNA was isolated, cRNA was made and hybridized to the U133A and U133B Affymetrix arrays (total of 24 arrays). No samples were pooled.
transcription profiling by array, disease state
Loss of ARNT/HIF1beta mediates altered gene expression and pancreatic-islet dysfunction in human type 2 diabetes. Gunton Jenny E, Kulkarni Rohit N, Yim SunHee, Okada Terumasa, Hawthorne Wayne J, Tseng Yu-Hua, Roberson Russell S, Ricordi Camillo, O'Connell Philip J, Gonzalez Frank J, Kahn C Ronald.