E-CBIL-11 - Transcription profiling of rat pancreatic beta-cells in response to double stranded RNA and IFNgamma exposure
Released on 20 June 2006, last updated on 21 February 2012
Viral infections and local production of IFNgamma might contribute to beta-cell dysfunction/death in Type 1 diabetes. Double stranded RNA accumulates in the cytosol of viral-infected cells, and exposure of purified rat beta cells to dsRNA (polyinosinic-polycytidylic acid, PIC) in combination with IFNgamma results in beta-cell dysfunction and apoptosis. To elucidate the molecular mechanisms involved in PIC + IFNgamma-effects, we determined the global profile of genes modified by these agents in primary rat beta cells. FACS-purified rat beta cells were cultured for 6 or 24 h in control condition or with IFNgamma, PIC or a combination of both agents. The gene expression profile was analyzed in duplicate with the Affymetrix RG U34A microarray.
compound treatment, time series, transcription profiling by array
Global profiling of double stranded RNA- and IFN-gamma-induced genes in rat pancreatic beta cells. Rasschaert J, Liu D, Kutlu B, Cardozo AK, Kruhoffer M, ORntoft TF, Eizirik DL.