E-BUGS-89 - Transcription profiling of by array of Streptococcus pneumoniae D39 to investigate the effect of manganese limitation

Status
Released on 22 July 2010, last updated on 1 May 2014
Organism
Streptococcus pneumoniae
Samples (2)
Array (1)
Protocols (6)
Description
The importance of Mn(2+) for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn(2+) is required are yet to be fully elucidated. Here, we analyzed the effect of Mn(2+) limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn(2+) transporter, pneumococcal surface antigen A (PsaA) with its isogenic wild-type counterpart. We provide clear evidence for the Mn(2+)-dependent regulation of expression of oxidative stress-response enzymes SpxB and Mn(2+)-SodA, and of virulence-associated genes pcpA and prtA. We also demonstrate the upregulation of at least one oxidative and nitrosative stress-response gene cluster, comprising adhC, nmlRsp and czcD, in response to Mn(2+) stress. A significant increase in 6-phosphogluconate dehydrogenase activity in the psaA mutant grown under Mn(2+)-replete conditions, and upregulation of an oligopeptide ABC permease (AppDCBA), was also observed. Together, transcriptomic and proteomic analyses provided evidence for Mn(2+) having a central role in activating or stimulating enzymes involved in central carbon and general metabolism. Our results also highlight the importance of high-affinity Mn(2+) transport by PsaA in pneumococcal competence, physiology and metabolism, and elucidate mechanisms underlying the response to Mn(2+) stress. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-89
Experiment types
transcription profiling by array, growth condition, strain or line
Contact
Citation
Central Role of Manganese in Regulation of Stress Responses, Physiology, and Metabolism in Streptococcus pneumoniae. Ogunniyi, Abiodun D.; Mahdi, Layla K.; Jennings, Michael P.; McEwan, Alastair G.; McDevitt, Christopher A.; Van der Hoek, Mark B.; Bagley, Christopher J.; Hoffmann, Peter; Gould, Katherine A.; Paton, James C.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-89.idf.txt
Sample and data relationshipE-BUGS-89.sdrf.txt
Raw data (1)E-BUGS-89.raw.1.zip
Processed data (1)E-BUGS-89.processed.1.zip
Experiment designE-BUGS-89.biosamples.png, E-BUGS-89.biosamples.svg
Array designA-BUGS-14.adf.txt
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