E-BUGS-89 - Transcription profiling of by array of Streptococcus pneumoniae D39 to investigate the effect of manganese limitation
Released on 22 July 2010, last updated on 1 May 2014
The importance of Mn(2+) for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn(2+) is required are yet to be fully elucidated. Here, we analyzed the effect of Mn(2+) limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn(2+) transporter, pneumococcal surface antigen A (PsaA) with its isogenic wild-type counterpart. We provide clear evidence for the Mn(2+)-dependent regulation of expression of oxidative stress-response enzymes SpxB and Mn(2+)-SodA, and of virulence-associated genes pcpA and prtA. We also demonstrate the upregulation of at least one oxidative and nitrosative stress-response gene cluster, comprising adhC, nmlRsp and czcD, in response to Mn(2+) stress. A significant increase in 6-phosphogluconate dehydrogenase activity in the psaA mutant grown under Mn(2+)-replete conditions, and upregulation of an oligopeptide ABC permease (AppDCBA), was also observed. Together, transcriptomic and proteomic analyses provided evidence for Mn(2+) having a central role in activating or stimulating enzymes involved in central carbon and general metabolism. Our results also highlight the importance of high-affinity Mn(2+) transport by PsaA in pneumococcal competence, physiology and metabolism, and elucidate mechanisms underlying the response to Mn(2+) stress. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-89
transcription profiling by array, growth condition, strain or line
Central Role of Manganese in Regulation of Stress Responses, Physiology, and Metabolism in Streptococcus pneumoniae. Ogunniyi, Abiodun D.; Mahdi, Layla K.; Jennings, Michael P.; McEwan, Alastair G.; McDevitt, Christopher A.; Van der Hoek, Mark B.; Bagley, Christopher J.; Hoffmann, Peter; Gould, Katherine A.; Paton, James C.