E-BUGS-86 - Transcription profiling by array of Mycobacterium tuberculosis virulent and attenuated strains infecting macrophages

Status
Released on 16 August 2010, last updated on 1 May 2014
Organism
Mycobacterium tuberculosis
Samples (5)
Array (1)
Protocols (11)
Description
H37Rv and H37Ra are well-described laboratory strains of Mycobacterium tuberculosis derived from the same parental strain, H37, that show dramatically different pathogenic phenotypes. In this study, the transcriptomes of the two strains during axenic growth in broth and during intracellular growth within murine bone-marrow macrophages were compared by whole genome expression profiling. We identified and compared adaptations made by either strain upon encountering an intracellular environment and also directly compared the transcriptomes of the two strains while inside macrophages. In the former comparison, both strains appeared to induce genes that would facilitate intracellular survival including those involved in mycobactin synthesis and fatty acid metabolism. However, this response was stronger and more extensive for H37Rv than for H37Ra. This was manifested as the differential expression of a greater number of genes and an increased magnitude of expression for some genes in H37Rv. In comparing intracellular transcriptomes, fifty genes were found to be differentially expressed between the strains. Of these fifty, twelve were found to be under control of the PhoPR regulon. Further differences between strains lay in the expression of genes whose products were members of the ESAT-6 family of proteins or were associated with their secretion. Along with the recent identification of single nucleotide polymorphisms in H37Ra when compared to H37Rv our demonstration of differential expression of PhoP-regulated and ESAT-6-like genes during macrophage infection further highlights the significance of these genes in the attenuation of H37Ra. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-86
Experiment types
transcription profiling by array, growth condition, strain or line
Contact
Citation
Contrasting transcriptional responses of a virulent and an attenuated strain of Mycobacterium tuberculosis infecting macrophages. Li AH, Waddell SJ, Hinds J, Malloff CA, Bains M, Hancock RE, Lam WL, Butcher PD, Stokes RW. PLoS One 5(6):e11066 (2010), PMID:20548782
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-86.idf.txt
Sample and data relationshipE-BUGS-86.sdrf.txt
Raw data (1)E-BUGS-86.raw.1.zip
Processed data (1)E-BUGS-86.processed.1.zip
Experiment designE-BUGS-86.biosamples.png, E-BUGS-86.biosamples.svg
Array designA-BUGS-23.adf.txt
Links