E-BUGS-32 - Transcription profiling of Yersinia pestis dam mutant that has attenuated virulence in mice

Status
Released on 30 January 2007, last updated on 1 May 2014
Organism
Yersinia pestis
Samples (2)
Array (1)
Protocols (7)
Description
We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-32
Experiment types
transcription profiling by array, genetic modification, replicate
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-32.idf.txt
Sample and data relationshipE-BUGS-32.sdrf.txt
Raw data (1)E-BUGS-32.raw.1.zip
Processed data (1)E-BUGS-32.processed.1.zip
Experiment designE-BUGS-32.biosamples.png, E-BUGS-32.biosamples.svg
Array designA-BUGS-11.adf.txt
Links