E-BUGS-2 - Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays
Released on 7 March 2007, last updated on 1 May 2014
Regulation of the expression of heat-shock proteins plays an important role in the pathogenesis of Mycobacterium tuberculosis. The heat-shock response of bacteria involves genome-wide changes in gene expression. A combination of targeted mutagenesis and whole-genome expression profiling was used to characterize transcription factors responsible for control of genes encoding the major heat-shock proteins of M.tuberculosis. Two heat-shock regulons were identified. HspR acts as a transcriptional repressor for the members of the Hsp70 (DnaK) regulon, and HrcA similarly regulates the Hsp60 (GroE)response. These two specific repressor circuits overlap with broader transcriptional changes mediated by alternative sigma factors during exposure to high temperatures. Several previously undescribed heat-shock genes were identified as members of the HspR and HrcA regulons. A novel HspR-controlled operon encodes a member of the low-molecular-mass a-crystallin family. This protein is one of the most prominent features of the M.tuberculosis heatshock response and is related to a major antigen induced in response to anaerobic stress. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-2
transcription profiling by array, genetic modification, growth condition, replicate, stimulus or stress
BuG@S group <email@example.com>, Graham Stewart, Lorenz Wernisch, Shirley Graham
Dissection of the heat-shock response in Mycobacterium tuberculosis using mutants and microarrays. Stewart GR, Wernisch L, Stabler R, Mangan JA, Hinds J, Laing KG, Young DB, Butcher PD.