E-BUGS-140 - Transcription profiling by array of Mycobacterium smegmatis response to nitrogen stress using bipartite active modules

Status
Released on 10 June 2013, last updated on 2 June 2014
Organism
Mycobacterium smegmatis
Samples (24)
Array (1)
Protocols (3)
Description
BACKGROUND: The ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown. RESULTS: A global transcriptional analysis of the mycobacterial response to nitrogen stress, showed a significant change in the differential expression of 16% of the Mycobacterium smegmatis genome. Gene expression changes were mapped onto the metabolic network using Active Modules for Bipartite Networks (AMBIENT) to identify metabolic pathways showing coordinated transcriptional responses to the stress. AMBIENT revealed several key features of the metabolic response not identified by KEGG enrichment alone. Down regulated reactions were associated with the general reduction in cellular metabolism as a consequence of reduced growth rate. Up-regulated modules highlighted metabolic changes in nitrogen assimilation and scavenging, as well as reactions involved in hydrogen peroxide metabolism, carbon scavenging and energy generation. CONCLUSIONS: Application of an Active Modules algorithm to transcriptomic data identified key metabolic reactions and pathways altered in response to nitrogen stress, which are central to survival under nitrogen limiting environments. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-140]
Experiment types
transcription profiling by array, stimulus or stress design, time series design
Contact
Citation
Deciphering the response of Mycobacterium smegmatis to nitrogen stress using bipartite active modules. Williams KJ, Bryant WA, Jenkins VA, Barton GR, Witney AA, Pinney JW, Robertson BD. BMC Genomics 14:436 (2013), Europe PMC 23819599
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-140.idf.txt
Sample and data relationshipE-BUGS-140.sdrf.txt
Raw data (1)E-BUGS-140.raw.1.zip
Array designA-BUGS-40.adf.txt
Links