E-BUGS-135 - Transcription profiling by array of adenylylation of mycobacterial Glnk (PII) protein induced by nitrogen limitation

Released on 10 May 2013, last updated on 2 June 2014
Mycobacterium tuberculosis
Samples (6)
Array (1)
Protocols (3)
PII proteins are pivotal regulators of nitrogen metabolism in most prokaryotes, controlling the activities of many targets, including nitrogen assimilation enzymes, two component regulatory systems and ammonium transport proteins. Escherichia coli contains two PII-like proteins, PII (product of glnB) and GlnK, both of which are uridylylated under nitrogen limitation at a conserved Tyrosine-51 residue by GlnD (a uridylyl transferase). PII-uridylylation in E. coli controls glutamine synthetase (GS) adenylylation by GlnE and mediates the NtrB/C transcriptomic response. Mycobacteria contain only one PII protein (GlnK) which in environmental Actinomycetales is adenylylated by GlnD under nitrogen limitation. However in mycobacteria, neither the type of GlnK (PII) covalent modification nor its precise role under nitrogen limitation is known. In this study, we used LC-Tandem MS to analyse the modification state of mycobacterial GlnK (PII), and demonstrate that during nitrogen limitation GlnK from both non-pathogenic Mycobacterium smegmatis and pathogenic Mycobacterium tuberculosis is adenylylated at the Tyrosine-51 residue; we also show that GlnD is the adenylyl transferase enzyme responsible. Further analysis shows that in contrast to E. coli, GlnK (PII) adenylylation in M. tuberculosis does not regulate GS adenylylation, nor does it mediate the transcriptomic response to nitrogen limitation. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-135]
Experiment types
transcription profiling by array, genotyping design, growth condition design
Adenylylation of mycobacterial Glnk (PII) protein is induced by nitrogen limitation. Williams KJ, Bennett MH, Barton GR, Jenkins VA, Robertson BD. :198-206 (2013), PMID:23352854
Adenylylation of mycobacterial Glnk (PII) protein is induced by nitrogen limitation. Williams KJ, Bennett MH, Barton GR, Jenkins VA, Robertson BD.
Investigation descriptionE-BUGS-135.idf.txt
Sample and data relationshipE-BUGS-135.sdrf.txt
Raw data (1)E-BUGS-135.raw.1.zip
Array designA-BUGS-41.adf.txt