E-BUGS-131 - Survival of Staphylococcus aureus ST398 in the Human Nose after Artificial Inoculation

Status
Released on 29 October 2012, last updated on 1 May 2014
Organism
Staphylococcus aureus
Samples (23)
Array (1)
Protocols (5)
Description
There is evidence that MRSA ST398 of animal origin is only capable of temporarily occupying the human nose, and it is therefore, often considered a poor human colonizer.We inoculated 16 healthy human volunteers with a mixture of the human MSSA strain 1036 (ST931, CC8) and the bovine MSSA strain 5062 (ST398, CC398), 7 weeks after a treatment with mupirocin and chlorhexidine-containing soap. Bacterial survival was studied by follow-up cultures over 21 days. The human strain 1036 was eliminated faster (median 14 days; range 2-21 days) than the bovine strain 5062 (median 21 days; range 7-21 days) but this difference was not significant (p = 0.065). The bacterial loads were significantly higher for the bovine strain on day 7 and day 21. 4/14 volunteers (28.6%) showed elimination of both strains within 21 days. Of the 10 remaining volunteers, 5 showed no differences in bacterial counts between both strains, and in the other 5 the ST398 strain far outnumbered the human S. aureus strain. Within the 21 days of follow-up, neither human strain 1036 nor bovine strain 5062 appeared to acquire or lose any mobile genetic elements. In conclusion, S. aureus ST398 strain 5062 is capable of adequately competing for a niche with a human strain and survives in the human nose for at least 21 days. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-131]
Experiment types
comparative genomic hybridization by array, strain or line
Contact
Citation
Survival of Staphylococcus aureus ST398 in the human nose after artificial inoculation. Slingerland BC, Tavakol M, McCarthy AJ, Lindsay JA, Snijders SV, Wagenaar JA, van Belkum A, Vos MC, Verbrugh HA, van Wamel WJ. PLoS One 7(11):e48896 (2012), PMID:23155425
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-131.idf.txt
Sample and data relationshipE-BUGS-131.sdrf.txt
Raw data (1)E-BUGS-131.raw.1.zip
Array designA-BUGS-38.adf.txt
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