E-BUGS-126 - Transcription profiling by array of Streptoccocus pneumoniae serotype 3 and serogroup 11 clinical isolates from both general and indigenous populations in Australia

Status
Released on 1 December 2011, last updated on 1 May 2014
Organism
Streptococcus pneumoniae
Samples (12)
Array (1)
Protocols (7)
Description
The capsular serotype has long been associated with the virulence of Streptococcus pneumoniae. Here we present an in-depth study of phenotypic and genetic differences between serotype 3 and serogroup 11 S. pneumoniae clinical isolates from both the general and indigenous populations of Australia. Both serotypes/groups included clonally unrelated strains with differences in well-known polymorphic virulence genes, such as nanA and pspA, as demonstrated by multilocus sequence typing and Western blot analysis. Nonetheless, the serotype 3 strains were consistently and significantly more virulent in mice than the serogroup 11 strains. Despite extensive genomic analysis, noncapsular genes common to one serotype/group but not the other were not identified. Nevertheless, following the conversion of a serotype 11A isolate to serotype 3 and subsequent analysis in an intranasal infection model, it was evident that both capsular and noncapsular factors determine the virulence phenotype in mice. However, it appears that these noncapsular factors vary from strain to strain. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-126
Experiment types
transcription profiling by array, strain or line
Contacts
BuG@S group <awitney@sgul.ac.uk>, Denise Waldron, Lauren McAllister
Citations
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-126.idf.txt
Sample and data relationshipE-BUGS-126.sdrf.txt
Raw data (1)E-BUGS-126.raw.1.zip
Processed data (1)E-BUGS-126.processed.1.zip
Array designA-BUGS-14.adf.txt
Links