E-BUGS-125 - Comparative genomic hybridization by array of Campylobacter jejuni invasion of intestinal epithelial cells under low oxygen co-culture conditions that reflect the in vivo environment

Status
Released on 2 April 2012, last updated on 1 May 2014
Organism
Campylobacter jejuni
Samples (5)
Array (1)
Protocols (7)
Description
Campylobacter jejuni infection often results in bloody, inflammatory diarrhea, indicating bacterial disruption and invasion of the intestinal epithelium. Whilst C. jejuni infection can be reproduced in vitro using intestinal epithelial cell (IEC) lines, low numbers of bacteria invading IECs do not reflect these clinical symptoms. Performing in vitro assays under atmospheric oxygen conditions is neither optimal for microaerophilic C. jejuni nor reflects the low oxygen environment of the intestinal lumen. A Vertical Diffusion Chamber (VDC) model system creates microaerobic conditions at the apical surface and aerobic conditions at the baso-lateral surface of cultured IECs producing an in vitro system that closely mimics in vivo conditions in the human intestine. Nine-fold increases in interacting and eighty-fold increases in intracellular C. jejuni 11168H wild-type strain bacteria were observed after 24 hours co-culture with Caco-2 IECs in VDCs with microaerobic conditions at the apical surface compared to aerobic conditions. Increased bacterial interaction was matched by an enhanced and directional host innate immune response, particularly an increased baso-lateral secretion of the pro-inflammatory chemokine IL-8. Analysis of the invasive ability of a non-motile C. jejuni 11168H rpoN mutant in the VDC model system indicates that motility is an important factor in the early stages of bacterial invasion. The first report of the use of a VDC model system for studying the interactions of an invasive bacterial pathogen with IECs demonstrates the importance of performing such experiments under conditions that represent the in vivo situation and will allow novel insights into C. jejuni pathogenic mechanisms. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-125]
Experiment types
comparative genomic hybridization by array, growth condition
Contact
Citation
Increase in Campylobacter jejuni Invasion of Intestinal Epithelial Cells under Low-Oxygen Coculture Conditions That Reflect the In Vivo Environment. Mills, Dominic C.; Gundogdu, Ozan; Elmi, Abdi; Bajaj-Elliott, Mona; Taylor, Peter W.; Wren, Brendan W.;Dorrell, Nick.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-125.idf.txt
Sample and data relationshipE-BUGS-125.sdrf.txt
Raw data (1)E-BUGS-125.raw.1.zip
Array designA-BUGS-9.adf.txt
Links