E-BUGS-123 - Transcription profiling by tiling array of Antimicrobial Treatment Improves Mycobacterial Survival in Non-Permissive Growth Conditions
Released on 28 February 2014, last updated on 2 June 2014
Mycobacterium bovis, Mycobacterium tuberculosis
Antimicrobials targeting cell wall biosynthesis are generally considered inactive against non-replicating bacteria. Paradoxically, we found that in non-permissive growth conditions exposure of Mycobacterium bovis BCG bacilli to such antimicrobials enhanced their survival. We identified a transcriptional regulator, Raas (for regulator of antimicrobial-assisted survival) encoded by bcg1279 (rv1219c) as being responsible for the observed phenomenon. Induction of this transcriptional regulator resulted in reduced expression of specific ATP-dependent efflux pumps and promoted long-term survival of mycobacteria, while its deletion accelerated bacterial death in non-permissive growth conditions in vitro and during macrophage or mouse infection. These findings have implications for the design of antimicrobial drug combination therapies for persistent infectious diseases such as tuberculosis. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-123]
transcription profiling by array, compound treatment, growth condition, reference, replicate
Antimicrobial Treatment Improves Mycobacterial Survival in Non-Permissive Growth Conditions. Turapov O1, Waddell SJ, Burke B, Glenn S, Sarybaeva A, Tudo G, Labesse G, Young DI, Young M, Andrew PW, Butcher PD, Cohen-Gonsaud M, Mukamolova GV.
Antimicrobial treatment improves mycobacterial survival in nonpermissive growth conditions. Turapov O, Waddell SJ, Burke B, Glenn S, Sarybaeva AA, Tudo G, Labesse G, Young DI, Young M, Andrew PW, Butcher PD, Cohen-Gonsaud M, Mukamolova GV. :2798-2806 (2014), PMID:24590482