E-BUGS-122 - Transcription profiling by array of Mycobacterium tuberculosis grown in the presence of ZnSO4

Status
Released on 27 January 2012, last updated on 1 May 2014
Organism
Mycobacterium tuberculosis
Samples (1)
Array (1)
Protocols (5)
Description
Mycobacterium tuberculosis thrives within macrophages by residing in phagosomes and preventing them from maturing and fusing with lysosomes. A parallel transcriptional survey of intracellular mycobacteria and their host macrophages revealed signatures of heavy metal poisoning. In particular, mycobacterial genes encoding heavy metal efflux P-type ATPases CtpC, CtpG, and CtpV, and host cell metallothioneins and zinc exporter ZnT1, were induced during infection. Consistent with this pattern of gene modulation, we observed a burst of free zinc inside macrophages, and intraphagosomal zinc accumulation within a few hours postinfection. Zinc exposure led to rapid CtpC induction, and ctpC deficiency caused zinc retention within the mycobacterial cytoplasm, leading to impaired intracellular growth of the bacilli. Thus, the use of P(1)-type ATPases represents a M. tuberculosis strategy to neutralize the toxic effects of zinc in macrophages. We propose that heavy metal toxicity and its counteraction might represent yet another chapter in the host-microbe arms race. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-122]
Experiment types
transcription profiling by array, compound treatment, replicate
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-122.idf.txt
Sample and data relationshipE-BUGS-122.sdrf.txt
Raw data (1)E-BUGS-122.raw.1.zip
Array designA-GEOD-13420.adf.txt
Links