E-BUGS-117 - Extracellular Matrix Formation Enhances the Ability of Streptococcus pneumoniae to Cause Invasive Disease

Status
Released on 20 April 2011, last updated on 1 May 2014
Organism
Streptococcus pneumoniae
Samples (2)
Array (1)
Protocols (9)
Description
Background. During infection, pneumococci exist mainly in sessile biofilms rather than in planktonic form, except during sepsis. However, relatively little is known about how biofilms contribute to pneumococcal pathogenesis. Methods. We performed a biofilm assay and mouse infection experiments on opaque and transparent variants of a clinical serotype 19F strain. Results. After 4 days incubation, scanning electron microscopy revealed that opaque biofilm bacteria produced an extracellular matrix, whereas the transparent variant did not. The opaque biofilm-derived bacteria translocated from the nasopharynx to the lungs and brain of mice, and showed 100-fold greater in vitro adherence to A549 cells. Microarray analysis of planktonic and sessile bacteria from transparent and opaque variants showed differential gene expression in two operons: the lic operon, involved in choline uptake, and in the two-component system, ciaRH. Mutants of these genes did not form an extracellular matrix, could not translocate from the nasopharynx to the lungs or the brain, and adhered poorly to A549 cells. Conclusions. We conclude that only the opaque phenotype is able to form extracellular matrix, and that the lic operon and ciaRH contribute to this process. We propose that during infection, extracellular matrix formation enhances the ability of pneumococci to cause invasive disease. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-117
Experiment types
transcription profiling by array, growth condition
Contacts
Citation
Extracellular matrix formation enhances the ability of Streptococcus pneumoniae to cause invasive disease. Trappetti C, Ogunniyi AD, Oggioni MR, Paton JC. PLoS One 6(5):e19844 (2011), PMID:21611130
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-BUGS-117.idf.txt
Sample and data relationshipE-BUGS-117.sdrf.txt
Raw data (1)E-BUGS-117.raw.1.zip
Processed data (1)E-BUGS-117.processed.1.zip
Array designA-BUGS-14.adf.txt
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