E-BUGS-108 - Genetic Variation in Spatio-Temporal Confined USA300 Community-Associated MRSA Isolates: A Shift from Clonal Dispersion to Genetic Evolution?

Released on 15 April 2011, last updated on 1 May 2014
Staphylococcus aureus
Samples (116)
Array (1)
Protocols (9)
Introduction Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) are increasingly isolated, with USA300-0114 being the predominant clone in the USA. Comparative whole genome sequencing of USA300 isolates collected in 2002, 2003 and 2005 showed a limited number of single nucleotide polymorphisms and regions of difference. This suggests that USA300 has undergone rapid clonal expansion without great genomic diversification. However, whole genome comparison of CA-MRSA has been limited to isolates belonging to USA300. The aim of this study was to compare the genetic repertoire of different CA-MRSA clones with that of HA-MRSA from the USA and Europe through comparative genomic hybridization (CGH) to identify genetic clues that may explain the successful and rapid emergence of CA-MRSA. Materials and Methods Hierarchical clustering based on CGH of 48 MRSA isolates from the community and nosocomial infections from Europe and the USA revealed dispersed clustering of the 19 CA-MRSA isolates. This means that these 19 CA-MRSA isolates do not share a unique genetic make-up. Only the PVL genes were commonly present in all CA-MRSA isolates. However, 10 genes were variably present among 14 USA300 isolates. Most of these genes were present on mobile elements. Conclusion The genetic variation present among the 14 USA300 isolates is remarkable considering the fact that the isolates were recovered within one month and originated from a confined geographic area, suggesting continuous evolution of this clone. Data is also available from http://bugs.sgul.ac.uk/E-BUGS-108
Experiment types
transcription profiling by array, reference, strain or line
Genetic variation in spatio-temporal confined USA300 community-associated MRSA isolates: a shift from clonal dispersion to genetic evolution?. Carpaij N, Willems RJ, Rice TW, Weinstein RA, Hinds J, Witney AA, Lindsay JA, Bonten MJ, Fluit AC. PLoS One 6(2):e16419 (2011), PMID:21326601
Investigation descriptionE-BUGS-108.idf.txt
Sample and data relationshipE-BUGS-108.sdrf.txt
Raw data (1)E-BUGS-108.raw.1.zip
Processed data (1)E-BUGS-108.processed.1.zip
Array designA-BUGS-17.adf.txt