A-MEXP-7 - EMBL Iron Chip v3
The 'IronChip' (Version 3.0) contains a selection of 300 genes that encode proteins directly involved in iron metabolism or involved in linked pathways such as Cu-metabolism, NO-metabolism or oxidative stress. We verified both ends of the sequence for all genes represented on our arrays. Each gene is spotted at least 6 times in different locations of the chip and many genes are represented by up to 5 independent cDNA clones. This redundancy, together with the use of positive (spike-in) controls and negative controls in every subgrid of the array, offers an excellent control system to assure high data quality. Redundancy of genes at different positions of the microarray also assures that small gene expression changes are recognized accurately.
Martina Muckenthaler (firstname.lastname@example.org)