A-MEXP-2321 - BlueGnome human CGH-SNP-8x60K custom microarrays
This is the BlueGnome custom CGH+SNP-8x60K human microarray (BlueGnome 142 Ltd., Cambridge, UK). The design was genome-wide, but targeted known disease genes and regions (design identification: MUNCH005). It was made up of 59,090 60mer oligonucleotides mapped to the human genome according to the Genome Reference Consortium Human Build 37 of February 2009 (GRCh37/hg19). The practical resolution for aCGH was appr. 200 kb corresponding to three consecutive deviating probes with average spacing of 66 kb. The design comprised 18% SNP-probes enabling the detection of stretches of homozygosity of 13 Mb or wider. Respective male or female genomic DNA of known haplotype derived from single individuals displaying no clinical phenotype was used as reference (SNPRef DNA, commercially not available, BlueGnome Ltd., Cambridge, UK).
Stefanie Bug (Stefanie.Bug@synlab.com)