A-MAXD-3 - Hiscock-Senecio-Array1

Spotting Method: cDNA inserts were PCR amplified, ethanol precipitated and resuspended in 1xGSSA spotting solution (Genetix Ltd.) prior to transfer to 384-well plates (V-bottom, Greiner) for use in array printing. Inserts were printed onto Amersham CodeLink slides in double-spot format using a BioRobotics MicroGrid II arraying robot. Printed slides were placed in a salt chamber for 24hr before being stored in dry, dust-free conditions until required for hybridisation.

Adhesion Method: Amine linking

Number Of Plates: 18

Availability: Ask

Array format for studies of allopolyploid origin of Senecio cambrensis. Contains ~1100 clones from each of S. squalidus/S. cambrensis/S. vulgaris capitulum bud and S. squalidus/S. cambrensis/S. vulgaris mature flower bud cDNA libraries. Features were primarily chosen anonymously, so sequence information will be filled in as target clones are identified and sequenced.

Origin: Non-commercial
Hiscock Plant Reproductive Biology Group, University of Bristol
All experiments done using A-MAXD-3: (E-MAXD-5, E-MAXD-7)
Array DesignA-MAXD-3.adf.txt