A-FPMI-3 - Pyxis Genomics CattleArray7800
Pyxis Genomics CattleArray7800, final version For reference see: Vet Immunol Immunopathol. 2005 May 15;105(3-4):235-45. A 7872 cDNA microarray and its use in bovine functional genomics. Everts RE, Band MR, Liu ZL, Kumar CG, Liu L, Loor JJ, Oliveira R, Lewin HA. Department of Animal Sciences, University of Illinois, 206 Edward R. Madigan Laboratory, 1201 West Gregory Drive, Urbana, IL 61801, USA. The strategy used to create and annotate a 7872 cDNA microarray from cattle placenta and spleen cDNA sequences is described. This microarray contains approximately 6300 unique genes, as determined by BLASTN and TBLASTX similarity search against the human and mouse UniGene and draft human genome sequence databases (build 34). Sequences on the array were annotated with gene ontology (GO) terms, thereby facilitating data analysis and interpretation. A total of 3244 genes were annotated with GO terms. The array is rich in sequences encoding transcription factors, signal transducers and cell cycle regulators. Current research being conducted with this array is described, and an overview of planned improvements in our microarray platform for cattle functional genomics is presented. PMID: 15808303 [PubMed - in process] Information from the Pyxis Genomics web site at
Contains more than 7000 gene targets selected cDNA clones printed in duplicate on a single glass slide The array elements were selected from cDNA clones derived from expressed sequence tags (ESTs) of bovine spleen and normalized placenta libraries. The ESTs representing distinct genes were determined via sequence similarity searches against human UniGene sequences using an E value of e10-5 as a threshold. The CattleArray7600™ contains positive expression controls: beta actin (ACTB); phosphoglycerate kinase 1; (PGK1); hypoxanthine phosphoribosyltransferase (HPRT) and beta-2-microglobulin (B2M). Two exogenous plant genes, soy rubisco small chain 1 (RBS1) and chlorophyll ab binding protein (CAB) can be used as negative or spiking controls.
Heather Wilson (email@example.com)
|All experiments done using A-FPMI-3: (E-FPMI-2, E-FPMI-3)|