NCBI BLAST+

• PROTEIN DATABASES
  • UniProt Knowledgebase (The UniProt Knowledgebase includes UniProtKB/Swiss-Prot and UniProtKB/TrEMBL)
  • UniProtKB/Swiss-Prot (The manually annotated section of UniProtKB)
  • UniProtKB/Swiss-Prot isoforms (The manually annotated isoforms of UniProtKB/Swiss-Prot)
  • UniProtKB/TrEMBL (The automatically annotated section of UniProtKB)
  • UniProtKB Reference Proteomes plus Swiss-Prot
  • UniProtKB COVID-19
  • UniProtKB Taxonomic Subsets
    • UniProtKB Archaea
    • UniProtKB Arthropoda
    • UniProtKB Bacteria
    • UniProtKB Complete Microbial Proteomes
    • UniProtKB Eukaryota
    • UniProtKB Fungi
    • UniProtKB Human
    • UniProtKB Mammals
    • UniProtKB Nematoda
    • UniProtKB Reference Proteomes
    • UniProtKB Rodents
    • UniProtKB Vertebrates
    • UniProtKB Viridiplantae
    • UniProtKB Viruses
    • UniProtKB Enzyme
    • UniProtKB COVID-19
  • UniProt Clusters
    • UniProt Clusters 100% (UniRef100)
    • UniProt Clusters 90% (UniRef90)
    • UniProt Clusters 50% (UniRef50)
  • Patents
    • EPO Patent Protein Sequences
    • JPO Patent Protein Sequences
    • KIPO Patent Protein Sequences
    • USPTO Patent Protein Sequences
    • NR Patent Proteins Level-1
    • NR Patent Proteins Level-2
  • Structures
    • Protein Structure Sequences (PDBe protein structure sequences)
    • AlphaFold DB
    • UniProtKB PDB
  • EnsemblCOVID19
    • Sars_cov_2.ASM985889v3.pep.all
  • Other Protein Databases
    • Enzyme Portal (Sequences from enzyme reports from UniProtKB, PDBe, ChEMBL, ChEBI, REACTOME, Rhea and IntEnz)
    • UniProt Archive (Sequences from the UniProt Archive UniParc)
    • IntAct (Sequences from IntAct interactors)
    • IPD-IMGT/HLA (Immuno Polymorphism Database-International Immunogenetics project/Human Leucocyte Antigen)
    • IPD-KIR (Immuno Polymorphism Database-Killer-cell Immunoglobulin-like Receptors)
    • IPD-NHKIR (Immuno Polymorphism Database-Killer-cell Immunoglobulin-like Receptors)
    • IPD-MHC (Immuno Polymorphism Database-Major Histocompatibility Complex)
    • MEROPS-MPRO (Sequences from the MEROPS scan dataset)
    • MEROPS-MPEP (Sequences from the peptidase or inhibitor domain sequence only)
    • MEROPS-MP (Sequences from the full MEROPS collection)
    • ChEMBL (Sequences from a manually curated database of bioactive molecules with drug-like properties)

Enter or paste a PROTEIN DNA/RNA sequence in any supported format:

or upload a file: Use a example sequence | Clear sequence | See more example inputs

• PROGRAM
  blastp blastx

• INCLUDED TAXONOMY IDS
• EXCLUDED TAXONOMY IDS

• MATRIX
  BLOSUM45 BLOSUM50 BLOSUM62 BLOSUM80 BLOSUM90 PAM30 PAM70 PAM250
• MATCH/MISMATCH SCORES
  N/A
• GAP OPEN
  -1 (default) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 25
• GAP EXTEND
  -1 (default) 0 1 2 3 4
• EXP. THR
  1e-200 1e-100 1e-50 1e-10 1e-5 1e-4 1e-3 1e-2 1e-1 1.0 10 (default) 100 1000
• FILTER
  no yes
• DROPOFF
  0 (default) 2 4 6 8 10
• SCORES
  0 5 10 20 50 (default) 100 150 200 250 500 750 1000
• ALIGNMENTS
  0 5 10 20 50 (default) 100 150 200 250 500 750 1000
• SEQUENCE RANGE
• HSPS
• GAPALIGN
  true false
• ALIGNMENT VIEWS
  pairwise Query-anchored identities Query-anchored non-identities Flat query-anchored identities Flat query-anchored non-identities BLASTXML Tabular Tabular with comment lines Text ASN.1 Binary ASN.1 Comma-separated values BLAST archive format (ASN.1) Tabular with comment lines with btop
• COMPOSITION-BASED STATISTICS
  F (default) D 1 2 3
• WORD SIZE
• TRANSLATION TABLE
  N/A Standard SGC0 Vertebrate Mitochondrial Yeast Mitochondrial Mold Mitochondrial Protozoan Mitochondrial Coelenterate Invertebrate Mitochondrial Ciliate Nuclear Dasycladacean Nuclear Hexamita Nuclear Echinoderm Mitochondrial Flatworm Mitochondrial Euplotid Nuclear Bacterial and Plant Plastid Alternative Yeast Nuclear Ascidian Mitochondrial Alternative Flatworm Mitochondrial Blepharisma Macronuclear Chlorophycean Mitochondrial Trematode Mitochondrial Scenedesmus obliquus Mitochondrial Thraustochytrium Mitochondrial