Multiple Sequence Alignment
MUSCLE stands for MUltiple Sequence Comparison by Log- Expectation. MUSCLE is claimed to achieve both better average accuracy and better speed than ClustalW2 or T-Coffee, depending on the chosen options.
Important note: This tool can align up to 500 sequences or a maximum file size of 1 MB.
If you use this service, please consider citing the following publication: Search and sequence analysis tools services from EMBL-EBI in 2022
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