Multiple Sequence Alignment (MSA) is generally the alignment of three or more biological sequences (protein or nucleic acid) of similar length. From the output, homology can be inferred and the evolutionary relationships between the sequences studied.

By contrast, Pairwise Sequence Alignment tools are used to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences.

Clustal Omega

New MSA tool that uses seeded guide trees and HMM profile-profile techniques to generate alignments. Suitable for medium-large alignments.

Launch Clustal Omega

Kalign

Very fast MSA tool that concentrates on local regions. Suitable for large alignments.

Launch Kalign

MAFFT

MSA tool that uses Fast Fourier Transforms. Suitable for medium-large alignments.

Launch MAFFT

MUSCLE

Accurate MSA tool, especially good with proteins. Suitable for medium alignments.

Launch MUSCLE

MView

Transform a Sequence Similarity Search result into a Multiple Sequence Alignment or reformat a Multiple Sequence Alignment using the MView program.

Launch MView

T-Coffee

Consistency-based MSA tool that attempts to mitigate the pitfalls of progressive alignment methods. Suitable for small alignments.

Launch T-Coffee

WebPRANK

The EBI has a new phylogeny-aware multiple sequence alignment program which makes use of evolutionary information to help place insertions and deletions.
Try it out at WebPRANK.

The tools described on this page are provided using The EMBL-EBI search and sequence analysis tools APIs in 2019

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