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On the 1st December the majority of services hosted on www.ebi.ac.uk will be served over HTTPS by default. Services that are becoming HTTPS by default will automatically redirect users accessing the site on insecure HTTP URLs to secure HTTPS URLs.
Users of EMBL-EBI services may wish to update links, bookmarks or API clients to use the HTTPS URLs.


Service Retirement

Wise2DBA and Promoterwise are scheduled for retirement on 15th April 2018. Alternatives can be found at Exonerate, BWA or BLAT. If you have any concerns, please contact us via support.

Selected EMBOSS tools for sequence analysis

Pairwise Sequence Alignment

Needle More Information & Help Documentation

Create an optimal global alignment of two sequences using the Needleman-Wunsch algorithm

Protein Nucleotide

Stretcher More Information & Help Documentation

Improved version of the Needleman-Wunsch algorithm that allows larger sequences to be globally aligned

Protein Nucleotide

Water More Information & Help Documentation

Use the Smith-Waterman algorithm to calculate the local alignment of two sequences

Protein Nucleotide

Matcher More Information & Help Documentation

Identify local similarities between two sequences using a rigorous algorithm based on the LALIGN application

Protein Nucleotide

Sequence Translation

Transeq More Information & Help Documentation

Translate nucleic acid sequences to the corresponding peptide sequences

Launch Transeq

Sixpack More Information & Help Documentation

Display DNA sequences with 6-frame translation and ORFs

Launch Sixpack

Backtranseq More Information & Help Documentation

Back-translate protein sequences to nucleotide sequences

Launch Backtranseq

Backtranambig More Information & Help Documentation

Back-translate protein sequences to ambiguous nucleotide sequences

Launch Backtranambig

Sequence Statistics

Pepinfo More Information & Help Documentation

Create a variety of plots that display different amino acid properties, such as hydropathy or charged residues, and their position in the sequence

Launch Pepinfo

Pepstats More Information & Help Documentation

Calculate properties of protein sequences such as molecular weight

Launch Pepstats

Pepwindow More Information & Help Documentation

Draw a hydropathy plot for protein sequences

Launch Pepwindow

Cpgplot More Information & Help Documentation

Identify and plot CpG islands in nucleotide sequence(s)

Launch Cpgplot

Newcpgreport More Information & Help Documentation

Identify CpG islands in nucleotide sequence(s)

Launch Newcpgreport

Isochore More Information & Help Documentation

Plot isochores in DNA sequences

Launch Isochore

Sequence Format Conversion

Seqret More Information & Help Documentation

Read and Reformat biosequences.

Launch EMBOSS Seqret

The tools described on this page are provided using the EMBL-EBI bioinformatics web and programmatic tools framework. If you have any feedback or encounter any issues please let us know via EMBL-EBI support.