Dbfetch
LOCUS NS_000193 5701 bp DNA circular ENV 28-FEB-2013 DEFINITION Uncultured Termite group 1 bacterium phylotype Rs-D17 plasmid pTGRD2 DNA, complete sequence. ACCESSION NS_000193 VERSION NS_000193.1 DBLINK Project: 59059 BioProject: PRJNA59059 KEYWORDS ENV; RefSeq; isolate genome. SOURCE uncultured Termite group 1 bacterium phylotype Rs-D17 ORGANISM uncultured Termite group 1 bacterium phylotype Rs-D17 Bacteria; Elusimicrobia; environmental samples. REFERENCE 1 AUTHORS Hongoh,Y., Sharma,V.K., Prakash,T., Noda,S., Taylor,T.D., Kudo,T., Sakaki,Y., Toyoda,A., Hattori,M. and Ohkuma,M. TITLE Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell JOURNAL Proc. Natl. Acad. Sci. U.S.A. 105 (14), 5555-5560 (2008) PUBMED 18391199 REFERENCE 2 (bases 1 to 5701) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (06-JUN-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 5701) AUTHORS Toyoda,A., Hongoh,Y., Sharma,V.K., Hattori,M., Ohkuma,M. and Sakaki,Y. TITLE Direct Submission JOURNAL Submitted (01-OCT-2007) Contact:Yuichi Hongoh RIKEN, Ecomolecular Biorecycling Science Research Team; Hirosawa 2-1, Wako, Saitama 351-0198, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AP009512. [WARNING] On Feb 28, 2013 this sequence was replaced by NC_020421.1. The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..5701 /organism="uncultured Termite group 1 bacterium phylotype Rs-D17" /mol_type="genomic DNA" /isolation_source="a single cell of termite (Reticulitermes speratus) gut protist" /host="Trichonympha agilis" /db_xref="taxon:471821" /environmental_sample /plasmid="pTGRD2" /note="rRNA phylotype Rs-D17" gene 1..1254 /locus_tag="TGRD_P2-1" /db_xref="GeneID:6372915" CDS 1..1254 /locus_tag="TGRD_P2-1" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956715.1" /db_xref="GeneID:6372915" /translation="MWNEIITELKEKIPTEWLEPIKEESFKDDILTLAVPDTYYAQKY ETDYKPLIQNILKAKTGKAVGLQFQIVASKLLAEPVIKDEKPKTQLMKKTKFNGKPYF ENPAEFVSIAKAASQVKETDIKNAELTSMPNKYGITNVASFSLLDSKFFTYPNDKRKK TKVEMPVRFSNGVVKVYNLYRGQFAINDNGYGQLTTTHAKIFFAIIHIWQKQNSRYAD NKGFYAVVDISMRELAKQLGYQKVSGADYRRLLQRVKELVDFPMILSDGRVAHTFTFL NSAIGRTVHQSGKNKLMLRLTLNPFISKQFYERNVILRNPQCYKIKNPTAFKFLLCYD KRVVKGNNLKLNIYEVANDLELNISKLCHGVSVLKVAFRELNGYELNDSYRLYVELVK EEREWIVIAERISKEKQQPLKALLA" gene 2656..4302 /locus_tag="TGRD_P2-2" /db_xref="GeneID:6372916" CDS 2656..4302 /locus_tag="TGRD_P2-2" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956716.1" /db_xref="GeneID:6372916" /translation="MKLKKALCVYLTIVLFLTGCENLKSQIRGNQNTVASADQPTGDI IKSDNSGIIEKQKEKESDLKKIENNVDNPNGDNGGDNNGDNPNGDNGGDNPNGDNGGD NNGDSSQHKSSSEANGKDLRGWLWDHKGSISGIVLASAVVIGAIGFWGGQPNVCAVNY FYVPQAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQEN QAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQ GNQNPNLVYFDIHSGRYIPGNQNPNLVYFDIHSGRYIPIVRGNPNPILVVQGNLYQVQ GNESPFPVLLVEENQVQVPAVQELPSQQQLQRIQNRLDQLQGQLRNLESHAIFQFQQI QVEAEALQLHNEQYIQLIGSIVDDPLYPQLNLQFQQICQILRHSHSLQLYDIGFHIFL MREILDHHLHLTARNLPDVYGPPPRWEALRVQEQLERHNQQLNQLYQQIEGRLVIEQS VLGARPDSQQRQQELREFNELAIQLQEHQRRIEALLKWLI" gene 4708..5229 /locus_tag="TGRD_P2-3" /db_xref="GeneID:6372917" CDS 4708..5229 /locus_tag="TGRD_P2-3" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_001956717.1" /db_xref="GeneID:6372917" /translation="MTAVKFKKIFAVILMLSVFLSGCQRQVITGVSKNENDVSTSTNT PVPEPTPTPTPAPEPTPTPTPVPEPTPTPTPVPEPTPTPTPVPEPTPTPTPVPEPTPT PTPVPEPTPAFMDCFPKYEAPLSTPAPLDEDDYYDNRVKWALSHVDDDGNWVNGKHSE APKCLLVDYYNKF" ORIGIN 1 gtgtggaatg aaatcattac agaattaaaa gaaaaaatac cgactgaatg gcttgaacca 61 ataaaagagg aaagctttaa agacgatata ttaacgcttg ctgttcctga tacatattat 121 gctcaaaagt atgaaaccga ttataaacca ttaattcaaa atatactaaa agcaaaaaca 181 ggcaaagccg tcggcttgca atttcaaatt gttgcttcta aattactggc agagcctgtt 241 ataaaagacg aaaagccaaa aacgcaatta atgaaaaaaa cgaagtttaa cggcaagcca 301 tattttgaaa atcctgcgga gtttgtctca attgcaaaag ccgcaagtca ggtaaaagag 361 acagatatta aaaatgccga attaacttca atgcctaata aatatggcat aactaatgtt 421 gcttctttca gtttgcttga tagtaagttt tttacttatc cgaatgataa aaggaaaaaa 481 acgaaagttg aaatgcctgt aaggtttagt aatggtgttg ttaaggtata taatttgtat 541 cgagggcaat tcgctattaa cgataatggt tatggtcagt tgacaactac gcacgcaaaa 601 atattttttg cgattattca tatatggcaa aagcagaata gcagatatgc tgataataag 661 ggcttttatg cagttgttga tatttcaatg cgggaacttg caaagcagtt gggttatcaa 721 aaagtaagcg gagctgatta ccgcagatta ttgcaaagag ttaaagagct ggttgatttt 781 cctatgatac tttcagacgg acgtgttgct catactttta cttttttgaa tagtgctatt 841 ggtcgtactg ttcatcaaag cggaaagaat aaattaatgc taagacttac tcttaatcct 901 tttatttcaa aacaatttta cgaaagaaat gtcattttaa gaaatcctca atgctataag 961 attaaaaatc ctaccgcttt taaattcttg ttatgttatg ataaaagagt agttaaaggt 1021 aataacttaa aattaaatat atatgaagtt gcgaatgatt tggaattaaa tatatctaag 1081 ctttgtcacg gagtttctgt gttaaaagtt gcatttcgtg agttaaatgg atacgaactt 1141 aacgatagtt atcgcttgta tgtagaatta gttaaagagg aaagggaatg gattgtcatt 1201 gctgaacgta tctcaaaaga aaaacaacaa ccattaaaag cgttattagc ttaactgcag 1261 aactgacata gaatgcgaag cataagagga gcgaagctcc gtgcttgtag ttgtctttcc 1321 tctcttaaaa gcctgccttt taaaaaagct caaatctaaa agtaaaaata gtcatattaa 1381 acattgtcta aaatcttgtc tttaaaaaac aatgaaaaaa atctgttgtc gttgatgttc 1441 tctggaacac ccttgaggct gttcagtaga agtagcggta ttcccgctta tttattgtct 1501 tgtctttaaa atctattctg tttactgtta aaacgtaaaa aacgattaaa aatcgtaaaa 1561 actgcgttaa aggcacgctg gtgcctcaaa taacgcaaaa cagacgttcg ctggcataat 1621 agccattaaa aacatcttaa aatgtcaaaa tatgccaata ttatgccaaa ataaggcatt 1681 atcacatagt ctaaagcctt aaataaaaca aaataaaaaa aataaaaaag gctaaatgac 1741 tgtgataagc cttgaaaagc ttgtaaaagc taaaaaaaac ggatgtaaat ccgtcagacg 1801 gactgtaaaa aagagctggc taaaactaaa gccagcgata aagcgtgcgt ataaagactt 1861 gattgtatta gcacgctgtc cacattgttt ttgattgttg tgaaaaaatg tgggaaaaaa 1921 cttatgtttt aaaatgtaaa agattggtaa aaaaaaatat tggaaagtct ctaaaaggtg 1981 ataactgatt gtttaatact agtacttgaa aaacgcaata aaatgcgttc cagcctgtct 2041 ttaaaatcta atctctaaaa taatataagc ctatgattaa atattatagg cgtttcaaat 2101 tgctccttct tgatatgcaa ttgggacgtt gtctcttttt gctttaatat gcaattggct 2161 gactgacaac aaaataataa cgatagttta tgaaagcgta aaaagctaag acgttatgat 2221 aatgcttagt cgctgttgta aatatgtcta aaaatttatt atatatttat aggcttgcac 2281 gacatttaga taacgtctgt gatgtataaa tagtcaaaag ccgtcttaaa gtagacggca 2341 aaaataaaca aaaaatgagc gaagcgaaca ccctctatac ttgatataca tatatgctac 2401 gtggcgacaa aaagagccgt aaaaagttat aaaataataa ttactttctt aatcgttttc 2461 agctgaaaac gaaacagtat aaacttgata tactatatgc tagttgcaga caaaaaaaaa 2521 tgaaaaaaag attaagaaaa aaagtgaaaa aaattcagcc ccaaaaatag acccgaataa 2581 aacttatctg ctttgttata caaacttacc ttgagataca cacaaaaaaa tatctggaca 2641 gtttaaagaa aaaaaatgaa attgaaaaaa gctttatgcg tatatttaac gattgttctg 2701 tttttgacag gctgtgaaaa tcttaaaagc cagataagag gcaatcagaa tactgtcgct 2761 tctgccgacc agccgacagg cgatattata aaaagcgata atagtggaat aattgaaaag 2821 cagaaagaaa aagagtcaga tttaaaaaaa atagagaata atgtcgataa tccaaatggt 2881 gataacggtg gcgataataa tggcgataat ccaaatggtg ataacggtgg cgataatcca 2941 aatggtgata acggtggcga taataatggc gatagtagtc aacacaaatc gtccagcgaa 3001 gcgaatggaa aagatttaag aggatggtta tgggatcata aaggaagtat ttcaggtata 3061 gtattagcga gtgcggtagt tataggtgcg ataggatttt ggggtggaca accaaatgtt 3121 tgcgcagtta attactttta tgttccccaa gcagttcagg aaaatcaagc ccaagttcaa 3181 gttccagcag ttcaggaaaa tcaagcccaa gttcaagttc cagcagttca ggaaaatcaa 3241 gctcaagttc aagttccagc agttcaggaa aatcaagctc aagttcaagt tccagcagtt 3301 caggaaaatc aagctcaagt tcaagttcca gcagttcagg aaaatcaagc tcaagttcaa 3361 gttccagcag ttcaggaaaa tcaagcccaa gttcaagttc cagcagttca ggaaaatcaa 3421 gctcaagttc aagttccagc agttcaggaa aatcaagccc aagttcaagt tccagcagtt 3481 cagggaaatc aaaacccaaa tctagtttat tttgacatcc acagcggcag atatattcca 3541 ggaaatcaaa acccaaatct agtttatttt gacatccaca gcggcagata tattccaata 3601 gttcggggaa atccaaaccc aattctagta gttcagggaa atctatacca agttcaggga 3661 aatgaaagcc catttccagt tctactagtt gaggaaaatc aagttcaagt tccagcagtt 3721 caggaactac catcacaaca acaacttcaa cgaatacaaa atcgactaga tcaactacaa 3781 ggacaattac gaaatttaga atctcatgca atttttcaat ttcaacaaat tcaggtagag 3841 gcagaggcat tgcagctaca caatgaacaa tacattcagc tcattggatc tatagttgat 3901 gacccacttt atccgcaact aaacttacaa ttccagcaaa tatgtcaaat actgcgacac 3961 agccattccc tccaattata tgatattgga tttcatatat ttttaatgcg tgaaatactc 4021 gaccaccacc tacacctaac cgcccgaaac ctacctgatg tttatggacc ccctccacga 4081 tgggaagcac tgagagtaca ggaacaactt gaacgacaca atcaacaact aaaccaacta 4141 taccaacaaa tagaaggcag attagttatc gaacagagtg tgctaggagc tcgccccgat 4201 tcccaacaac gccaacaaga gctaagggaa tttaatgaac tagctattca actgcaagaa 4261 caccagcgac gtatagaagc attattgaag tggcttatat aaagcgaaaa ataaaaaaga 4321 atttgattac tgtgaaaacg atatgcgtca tactgctgac gctctaaaac tttgaaaaat 4381 atttgttcaa atagtatccg tatcagcaag gatacaggca ttataactct tacttgacta 4441 tataatgccc atccgtacca cctttgaggc aaaaaatgca cccacgttta gaccctgcaa 4501 aaatatattt agtccagtca aatagtgata attctcttaa tctggaggca gagatattct 4561 tatgaaaaaa actgataata aagataataa agataaaccc ataggaaatg catttcctat 4621 taacaatccg tttagaagtt ctagaactgt tcaaaaagct ttaaaaataa ttaataaaaa 4681 agttgttaaa aaagattaaa tgaggatatg actgcagtga aatttaaaaa aatatttgct 4741 gtgattttaa tgttgtctgt gttcttgtct ggctgtcaga ggcaggttat aacaggcgtt 4801 tctaaaaatg aaaatgatgt ttcaacttca acgaatactc ctgttccaga gcctactcct 4861 actccgactc ctgctccaga gcctactcct actccgactc ctgttccaga gcctactcct 4921 actccgactc ctgttccaga gcctactcct actccgactc ctgttccaga gcctactcct 4981 actccgactc ctgttccaga gcctactcct actccgactc ctgttccaga gcctactcct 5041 gcctttatgg attgtttccc taaatatgaa gccccattgt ctactcctgc tccattggat 5101 gaggatgatt attatgataa cagggtaaaa tgggctctat cgcacgttga tgatgatggt 5161 aactgggtta atggtaagca ttccgaagct cctaagtgct tgttagtaga ctattacaat 5221 aagttttaaa aataaggtaa gctttccgaa gctcctaagc gcttgttatt attaaaggat 5281 ttgtaatttt cagcgtctac tcctgctcct acttcaccat agagattacg tctcaagaca 5341 aactcaatta attattaagt tataaattat gaaaaagctt gcataaatgc aggctctttt 5401 ttttgtgttg acttaaacgt tagtcctttg ttagtatatg tgtttctatg tgctgataca 5461 cgtgtttcta tgtgctgata cacgtgtttc tatgtgctga tacacgtgtt tctatgtgct 5521 gatacacgtg tttctatgtg ctgatttttt gaaaaaagtg gctataaata aggattgggc 5581 ttactctcta tttctaacta ctctattact actatgtctg cctaaagtct ggcagacaat 5641 tttctttttg aaattttaaa agataaaaaa gacaaaaaac caaagaaata aaggataaaa 5701 a //