Dbfetch
LOCUS NC_004952 3990 bp DNA circular CON 24-FEB-2025 DEFINITION Francisella tularensis subsp. novicida F6168 plasmid pFNL10, complete sequence. ACCESSION NC_004952 VERSION NC_004952.1 DBLINK BioProject: PRJNA224116 BioSample: SAMN14224658 KEYWORDS RefSeq. SOURCE Francisella tularensis subsp. novicida F6168 ORGANISM Francisella tularensis subsp. novicida F6168 Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Thiotrichales; Francisellaceae; Francisella. REFERENCE 1 (bases 1 to 3990) AUTHORS Pomerantsev,A.P., Golovliov,I.R., Ohara,Y., Mokrievich,A.N., Obuchi,M., Norqvist,A., Kuoppa,K. and Pavlov,V.M. TITLE Genetic organization of the Francisella plasmid pFNL10 JOURNAL Plasmid 46 (3), 210-222 (2001) PUBMED 11735370 REFERENCE 2 (bases 1 to 3990) AUTHORS Pomerantsev,A.P., Golovliov,I.R., Mokrievish,A.N. and Pavlov,V.M. TITLE Direct Submission JOURNAL Submitted (20-JAN-1999) Microbiology, State Research Center for Applied Microbiology, Obolensk, Moscow Region 142279, Russia COMMENT REFSEQ INFORMATION: The reference sequence is identical to AF121418.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_009942045.2-RS_2025_02_24 Annotation Date :: 02/24/2025 01:40:53 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.9 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6 CDSs (total) :: 6 Genes (coding) :: 5 CDSs (with protein) :: 5 Genes (RNA) :: 0 tRNAs :: 0 ncRNAs :: 0 Pseudo Genes (total) :: 1 CDSs (without protein) :: 1 Pseudo Genes (ambiguous residues) :: 0 of 1 Pseudo Genes (frameshifted) :: 1 of 1 Pseudo Genes (incomplete) :: 0 of 1 Pseudo Genes (internal stop) :: 0 of 1 ##Genome-Annotation-Data-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..3990 /organism="Francisella tularensis subsp. novicida F6168" /mol_type="genomic DNA" /strain="F6168" /sub_species="novicida" /db_xref="taxon:1452728" /plasmid="pFNL10" gene complement(join(3934..3990,1..135)) /locus_tag="HKJ39_RS00005" CDS complement(join(3934..3990,1..135)) /locus_tag="HKJ39_RS00005" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_042536701.1" /translation="MNDFKKENRFLDLVKQQDDKFDDLTSKIDDLSKNIKSLRRVDLL CKIAFFSSIAILLFICLSS" gene complement(180..602) /locus_tag="HKJ39_RS00010" /old_locus_tag="pFNL10_p1" CDS complement(180..602) /locus_tag="HKJ39_RS00010" /old_locus_tag="pFNL10_p1" /inference="COORDINATES: ab initio prediction:GeneMarkS-2+" /note="Derived by automated computational analysis using gene prediction method: GeneMarkS-2+." /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="WP_010891134.1" /translation="MNIEIFKFSKKHKKINKKLIDLTKVKQVVTTGYSFIVVYQRGRK IELSYESSYWHAKKDLDNSNSDVGITYSYIKKDKRGPCHKDNYLRDSKGRLLKDWGKI EELHEKYPKIGKIWYISREETDKLLKDFTYKNGNLIMV" gene complement(605..1624) /locus_tag="HKJ39_RS00015" /old_locus_tag="pFNL10_p2" CDS complement(605..1624) /locus_tag="HKJ39_RS00015" /old_locus_tag="pFNL10_p2" /inference="COORDINATES: protein motif:HMM:NF013235.5" /GO_function="GO:0003887 - DNA-directed DNA polymerase activity [Evidence IEA]" /GO_process="GO:0006270 - DNA replication initiation [Evidence IEA]" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="replication initiation protein" /protein_id="WP_010891135.1" /translation="MKEVKNVNAGKEIAMSNTLVAGKYSLTKEEQNLIFLVASMVKRE DKEFHRYKISLSDLEKATGVKHNRVRLKQLMHSIMSKPVWLNKEQTKIANWFAYIEAD PKSSALICEFHWSLMPHMIQLQEYFTKAERQLLFSFKSKYSSRLYLLLKSKLGEQAGY ANIVDCVLYVDDMINDFDLPKSYSNRYSNFKNKFLLPALEEINQLSDIFVTYDDKDHH RKHGRKITNIKFTVSKVAETEEEFKQKLLDTKNKEDYIPIEASERLREVVLSDELDLD LYYVRSIFRHHHLHDIERVCDDTWRNWDNKKIKDRKRVFIANIKKLEKKPEENHKLPF GFEEV" gene 1995..2252 /locus_tag="HKJ39_RS00020" /old_locus_tag="pFNL10_p3" CDS 1995..2252 /locus_tag="HKJ39_RS00020" /old_locus_tag="pFNL10_p3" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_020292155.1" /GO_component="GO:0110001 - toxin-antitoxin complex [Evidence IEA]" /GO_function="GO:0003677 - DNA binding [Evidence IEA]; GO:0097351 - toxin sequestering activity [Evidence IEA]" /GO_process="GO:0045927 - positive regulation of growth [Evidence IEA]; GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="type II toxin-antitoxin system Phd/YefM family antitoxin" /protein_id="WP_011117071.1" /translation="MQTVNYSTFRNELSDSMDRVTKNHSPMIVTRGSKKEAVVMMSLE DFKAYEETAYLMRSMNNYKRLQNSIDEVESGLAIQKELIEE" gene 2249..2516 /locus_tag="HKJ39_RS00025" /old_locus_tag="pFNL10_p4" /pseudo CDS 2249..2516 /locus_tag="HKJ39_RS00025" /old_locus_tag="pFNL10_p4" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005556313.1" /GO_process="GO:0008219 - cell death [Evidence IEA]" /note="frameshifted; Derived by automated computational analysis using gene prediction method: Protein Homology." /pseudo /codon_start=1 /transl_table=11 /product="Txe/YoeB family addiction module toxin" gene 3075..3953 /locus_tag="HKJ39_RS00030" /old_locus_tag="pFNL10_p6" CDS 3075..3953 /locus_tag="HKJ39_RS00030" /old_locus_tag="pFNL10_p6" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017040200.1" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /codon_start=1 /transl_table=11 /product="integrase domain-containing protein" /protein_id="WP_011117073.1" /translation="MKDKAAKNRDFRKTILSVLQRNKDGSFATQANRKSILLQATKDL KKVGFSKVTAENFGNKHCYALRDHWRAQGLATATIKNRLACLRWLGEKMGKELPDNRK LEIENRKYSDNSINKAQEIDFKAISALTDRQALAIQLQREFGLRREESLKFQPSYAIK EHKIELKSSWTKVGRPREIPILNEKQRELLEKVKEVAGKGSLIESEKSYKQAMEHFTT RCQRAGIKNVHGFRHAYAQDRYRQLTGRECPKNGGLTSKQLTPEQKQQDYEARMTISE ELGHGREDVTVNYLGR" CONTIG join(AF121418.1:1..3990) //