NS_000193 5701 double DNA circular ENV 28-FEB-2013 06-JUN-2008 Uncultured Termite group 1 bacterium phylotype Rs-D17 plasmid pTGRD2 DNA, complete sequence NS_000193 NS_000193.1 ref|NS_000193.1| gnl|NCBI_GENOMES|23440 gi|189485775 PRJNA59059 ENV RefSeq isolate genome uncultured Termite group 1 bacterium phylotype Rs-D17 uncultured Termite group 1 bacterium phylotype Rs-D17 Bacteria; Elusimicrobia; environmental samples 1 1..5701 Hongoh,Y. Sharma,V.K. Prakash,T. Noda,S. Taylor,T.D. Kudo,T. Sakaki,Y. Toyoda,A. Hattori,M. Ohkuma,M. Complete genome of the uncultured Termite Group 1 bacteria in a single host protist cell Proc. Natl. Acad. Sci. U.S.A. 105 (14), 5555-5560 (2008) doi 10.1073/pnas.0801389105 18391199 2 1..5701 NCBI Genome Project Direct Submission Submitted (06-JUN-2008) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA 3 1..5701 Toyoda,A. Hongoh,Y. Sharma,V.K. Hattori,M. Ohkuma,M. Sakaki,Y. Direct Submission Submitted (01-OCT-2007) Contact:Yuichi Hongoh RIKEN, Ecomolecular Biorecycling Science Research Team; Hirosawa 2-1, Wako, Saitama 351-0198, Japan PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AP009512.; [WARNING] On Feb 28, 2013 this sequence was replaced by NC_020421.1.; The wood-feeding termite Reticulitermes speratus (Isoptera; Rhinotermitidae) was collected in Saitama, Japan. The entire gut was removed from a worker termite, and a single cell of the cellulolytic protist Trichonympha agilis (Parabasalia; Trichonymphida) in the gut was physically isolated by using a micromanipulator. In order to collect the intracellular symbionts of T. agilis, as-yet-uncultivated bacteria designated phylotype Rs-D17 belonging to the candidate division Termite Group 1, the host T. agilis cell was ruptured in buffer and bacterial cells which leaked out were collected with a glass capillary. These collected cells were directly subjected to isothermal whole genome amplification. After checking their purity by clone analysis of 16S rRNA gene and its adjacent ITS, the complete genome sequence was determined using ABI 3730 capillary sequencers and a GS20 system.; COMPLETENESS: full length. source 1..5701 1 5701 NS_000193.1 organism uncultured Termite group 1 bacterium phylotype Rs-D17 mol_type genomic DNA isolation_source a single cell of termite (Reticulitermes speratus) gut protist host Trichonympha agilis db_xref taxon:471821 environmental_sample plasmid pTGRD2 note rRNA phylotype Rs-D17 gene 1..1254 1 1254 NS_000193.1 locus_tag TGRD_P2-1 db_xref GeneID:6372915 CDS 1..1254 1 1254 NS_000193.1 locus_tag TGRD_P2-1 codon_start 1 transl_table 11 product hypothetical protein protein_id YP_001956715.1 db_xref GeneID:6372915 translation MWNEIITELKEKIPTEWLEPIKEESFKDDILTLAVPDTYYAQKYETDYKPLIQNILKAKTGKAVGLQFQIVASKLLAEPVIKDEKPKTQLMKKTKFNGKPYFENPAEFVSIAKAASQVKETDIKNAELTSMPNKYGITNVASFSLLDSKFFTYPNDKRKKTKVEMPVRFSNGVVKVYNLYRGQFAINDNGYGQLTTTHAKIFFAIIHIWQKQNSRYADNKGFYAVVDISMRELAKQLGYQKVSGADYRRLLQRVKELVDFPMILSDGRVAHTFTFLNSAIGRTVHQSGKNKLMLRLTLNPFISKQFYERNVILRNPQCYKIKNPTAFKFLLCYDKRVVKGNNLKLNIYEVANDLELNISKLCHGVSVLKVAFRELNGYELNDSYRLYVELVKEEREWIVIAERISKEKQQPLKALLA gene 2656..4302 2656 4302 NS_000193.1 locus_tag TGRD_P2-2 db_xref GeneID:6372916 CDS 2656..4302 2656 4302 NS_000193.1 locus_tag TGRD_P2-2 codon_start 1 transl_table 11 product hypothetical protein protein_id YP_001956716.1 db_xref GeneID:6372916 translation MKLKKALCVYLTIVLFLTGCENLKSQIRGNQNTVASADQPTGDIIKSDNSGIIEKQKEKESDLKKIENNVDNPNGDNGGDNNGDNPNGDNGGDNPNGDNGGDNNGDSSQHKSSSEANGKDLRGWLWDHKGSISGIVLASAVVIGAIGFWGGQPNVCAVNYFYVPQAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQENQAQVQVPAVQGNQNPNLVYFDIHSGRYIPGNQNPNLVYFDIHSGRYIPIVRGNPNPILVVQGNLYQVQGNESPFPVLLVEENQVQVPAVQELPSQQQLQRIQNRLDQLQGQLRNLESHAIFQFQQIQVEAEALQLHNEQYIQLIGSIVDDPLYPQLNLQFQQICQILRHSHSLQLYDIGFHIFLMREILDHHLHLTARNLPDVYGPPPRWEALRVQEQLERHNQQLNQLYQQIEGRLVIEQSVLGARPDSQQRQQELREFNELAIQLQEHQRRIEALLKWLI gene 4708..5229 4708 5229 NS_000193.1 locus_tag TGRD_P2-3 db_xref GeneID:6372917 CDS 4708..5229 4708 5229 NS_000193.1 locus_tag TGRD_P2-3 codon_start 1 transl_table 11 product hypothetical protein protein_id YP_001956717.1 db_xref GeneID:6372917 translation MTAVKFKKIFAVILMLSVFLSGCQRQVITGVSKNENDVSTSTNTPVPEPTPTPTPAPEPTPTPTPVPEPTPTPTPVPEPTPTPTPVPEPTPTPTPVPEPTPTPTPVPEPTPAFMDCFPKYEAPLSTPAPLDEDDYYDNRVKWALSHVDDDGNWVNGKHSEAPKCLLVDYYNKF 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gcctactcctactccgactcctgttccagagcctactcctgcctttatggattgtttccctaaatatgaagccccattgtctactcctgctccattggatgaggatgattattatgataacagggtaaaatgggctctatcgcacgttgatgatgatggtaactgggttaatggtaagcattccgaagctcctaagtgcttgttagtagactattacaataagttttaaaaataaggtaagctttccgaagctcctaagcgcttgttattattaaaggatttgtaattttcagcgtctactcctgctcctacttcaccatagagattacgtctcaagacaaactcaattaattattaagttataaattatgaaaaagcttgcataaatgcaggctcttttttttgtgttgacttaaacgttagtcctttgttagtatatgtgtttctatgtgctgatacacgtgtttctatgtgctgatacacgtgtttctatgtgctgatacacgtgtttctatgtgctgatacacgtgtttctatgtgctgattttttgaaaaaagtggctataaataaggattgggcttactctctatttctaactactctattactactatgtctgcctaaagtctggcagacaattttctttttgaaattttaaaagataaaaaagacaaaaaaccaaagaaataaaggataaaaa BioProject PRJNA59059