ID   H81802; SV 1; linear; mRNA; EST; HUM; 324 BP.
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AC   H81802;
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DT   16-NOV-1995 (Rel. 45, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 2)
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DE   ys68b09.s1 Soares retina N2b4HR Homo sapiens cDNA clone IMAGE:219929 3'
DE   similar to SP:VAV_HUMAN P15498 VAV ;, mRNA sequence.
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KW   EST.
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OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
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RN   [1]
RP   1-324
RA   Hillier L., Clark N., Dubuque T., Elliston K., Hawkins M., Holman M.,
RA   Hultman M., Kucaba T., Le M., Lennon G., Marra M., Parsons J., Rifkin L.,
RA   Rohlfing T., Soares M., Tan F., Trevaskis E., Waterston R., Williamson A.,
RA   Wohldmann P., Wilson R.;
RT   "The WashU-Merck EST Project";
RL   Unpublished.
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DR   MD5; 2d35eb643f25c2e2b700e9dcfd9172dd.
DR   UNILIB; 556; 302.
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CC   Contact: Wilson RK
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: est@watson.wustl.edu
CC   High quality sequence stops: 301
CC   Source: IMAGE Consortium, LLNL
CC   This clone is available royalty-free through LLNL ; contact the
CC   IMAGE Consortium (info@image.llnl.gov) for further information.
CC   Possible reversed clone: similarity on wrong strand
CC   Insert Length: 1065   Std Error: 0.00
CC   Seq primer: Promega -21m13
CC   High quality sequence stop: 301.
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FH   Key             Location/Qualifiers
FH
FT   source          1..324
FT                   /organism="Homo sapiens"
FT                   /lab_host="DH10B (ampicillin resistant)"
FT                   /mol_type="mRNA"
FT                   /sex="male"
FT                   /dev_stage="55 year old"
FT                   /clone_lib="Soares retina N2b4HR"
FT                   /clone="IMAGE:219929"
FT                   /tissue_type="retina"
FT                   /note="Organ: eye; Vector: pT7T3D (Pharmacia) with a
FT                   modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
FT                   strand cDNA was primed with a Not I - oligo(dT) primer [5'
FT                   TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
FT                   double-stranded cDNA was size selected, ligated to Eco RI
FT                   adapters (Pharmacia), digested with Not I and cloned into
FT                   the Not I and Eco RI sites of a modified pT7T3 vector
FT                   (Pharmacia). The retinas were obtained from a 55 year old
FT                   Caucasian  and total cellular poly(A)+ RNA was extracted 6
FT                   hrs after their removal. The retina RNA  was kindly
FT                   provided by Roderick R. McInnes M.D. Ph.D. from the
FT                   University  of Toronto. Library constructed by Bento Soares
FT                   and M.Fatima Bonaldo. "
FT                   /db_xref="taxon:9606"
FT                   /db_xref="UNILIB:556"
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SQ   Sequence 324 BP; 75 A; 79 C; 90 G; 78 T; 2 other;
     cagtcggcat tcctgcatcg catccggcag aacgtggccg actcagtgga gaagggcctc        60
     acggaggaga atgtcaaggt cctgttctcg aacatcgaag acatcctgga agttcataag       120
     gatttcttgg ccgccttgga gtattgttta cacccggagc cgcagtctca gcatgaactt       180
     gggaatgttt tcttaaaatt caaggacaag ttctgcgtgt acgaggagta ttgcagcaac       240
     catgagaaag ccctgaggct gcctggtgga gctgaacaag atccctaccg tgcgcgcctt       300
     ccttttnagc tgcatgcttn tggg                                              324
//