ID   BI095947; SV 1; linear; mRNA; EST; PLN; 706 BP.
XX
AC   BI095947;
XX
DT   25-JUN-2001 (Rel. 68, Created)
DT   25-JUN-2001 (Rel. 68, Last updated, Version 1)
XX
DE   PIP2D_G12 Sugar Beet germination cDNA library Beta vulgaris cDNA 5' similar
DE   to Arabidopsis hypothetical protein AC006284, mRNA sequence.
XX
KW   EST.
XX
OS   Beta vulgaris
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Betoideae; Beta.
XX
RN   [1]
RP   1-706
RA   de los Reyes B.G., McGrath J.M., Myers S.;
RT   "Differential gene expression in sugar beet seedlings (Beta vulgaris )
RT   germinated under stress conditions (de los Reyes,B.G., McGrath ,J.M.,
RT   Myers,S.)";
RL   Unpublished.
XX
DR   MD5; 18578227c4971418f3b605af743182c0.
DR   UNILIB; 14890; 4050.
XX
CC   Contact: J. Mitchell McGrath
CC   Sugar Beet Genetics Lab., USDA/ARS/Sugar Beet and Bean Research
CC   Unit
CC   Michigan State University
CC   494 Plant and Soil Sci. Bldg., East Lansing MI 48824, USA
CC   Tel: (517)-432-2355
CC   Fax: (517)-337-6782
CC   Email: mitchmcg@pilot.msu.edu
CC   Seq primer: T3.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..706
FT                   /organism="Beta vulgaris"
FT                   /lab_host="SOLR"
FT                   /cultivar="USH20"
FT                   /mol_type="mRNA"
FT                   /dev_stage="4-day germination under stress (salt/NaCl,
FT                   dehydration/Mannitol and anaerobic stress)"
FT                   /clone_lib="Sugar Beet germination cDNA library"
FT                   /tissue_type="whole seedlings"
FT                   /note="Organ: seeds; Vector: pBluescript II KS (+) excised
FT                   from Lambda Uni-Zap XR; Site_1: EcoRI; Site_2: XhoI; cDNAs
FT                   were derived from reverse transcription of pooled mRNA
FT                   samples  from stressed seedlings (germinated for 4 days in
FT                   150mM NaCl, 200mM  Mannitol, submerged in distilled water
FT                   and 0.3% Hydrogen peroxide).  The cDNA library was
FT                   generated by directional ligation of the cDNAs  in the
FT                   EcoRI and XhoI sites of Lambda Uni-Zap XR vector
FT                   (Stratagene).  The library was excised as a pBluescript
FT                   plasmid from Lambda Uni-Zap  XR by ExAssist helper phage
FT                   and plated on SOLR host cells. "
FT                   /db_xref="taxon:161934"
FT                   /db_xref="UNILIB:14890"
XX
SQ   Sequence 706 BP; 222 A; 153 C; 138 G; 192 T; 1 other;
     gcacgagggt aagacggagt ggagactttg agaggacgaa agaaggtgag aaacatcata        60
     ccaatggttt catctttgta ttcttccttc cagacctctt ctcttcaagt aataccatcc       120
     tcagtttcca cttttcacca ctcaaactca aagaaattgc agtccagaat ttacttttct       180
     gcaaatgtaa atccttgttt ttctaagctc tctcaacaag gattccaaaa atactgtcta       240
     cctttagctg tttctttagt actctcctgg tcaaatcctg ctcaagcaag atttttgtca       300
     agctctactg gaatagaatc tgttccaggt ccccaactgc cttctatcga attcctcgat       360
     cgcttcaatg aagagaacca aaagaagtat gcagctgatg atgaaagaat caaatcatcc       420
     cctatcatga aagagctcct tgagcgttcc aaaaagaaca aagacaagaa taagcaagaa       480
     atattggaca agtattgcat acgtggagcg gagtggggag tgggagattg ctcgacccaa       540
     gggatgacag cagaggagaa agataatttc attgcaatgc tgaagcagaa agcagctgct       600
     gctgctacca aatgatctaa ctntccttat atctttcagt gagccatagc catgatgtac       660
     tacagtacca gttgtgaaga tcgatctttc gcacaagtaa tatcat                      706
//