Dbfetch

ID   MP923679; SV 1; linear; other DNA; PAT; SYN; 6199 BP.
XX
AC   MP923679;
XX
DT   17-MAR-2021 (Rel. 144, Created)
DT   17-MAR-2021 (Rel. 144, Last updated, Version 1)
XX
DE   Sequence 171 from Patent WO2021023982.
XX
KW   .
XX
OS   synthetic construct
OC   other sequences; artificial sequences.
XX
RN   [1]
RA   MCCORMICK A.J., ATKINSON N.J.;
RT   "PYRENOID-LIKE STRUCTURES";
RL   Patent number WO2021023982-A1/171, 11-FEB-2021.
RL   UNIV COURT UNIV OF EDINBURGH [GB].
XX
DR   MD5; c3e5e82ed8c7750da726dc8f14624c64.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..6199
FT                   /organism="synthetic construct"
FT                   /mol_type="other DNA"
FT                   /note="Synthetic Construct"
FT                   /db_xref="taxon:32630"
FT   misc_feature    1..718
FT                   /note="ocs terminator"
FT   misc_feature    715..718
FT                   /note="MoClo overhang in ocs terminator"
FT   misc_feature    719..1624
FT                   /note="turbo GFP (Pontellina pluma)"
FT   misc_feature    1622..1624
FT                   /note="MoClo overhang in turbo GFP (Pontellina pluma)"
FT   misc_feature    1625..2497
FT                   /note="mature EPYC1"
FT   misc_feature    1625
FT                   /note="MoClo overhang in mature EPYC1"
FT   misc_feature    2498..2669
FT                   /note="Rbcs1A transit peptide"
FT   misc_feature    2498..2501
FT                   /note="MoClo overhang in Rbcs1A transit peptide"
FT   misc_feature    2666..2669
FT                   /note="MoClo overhang in Rbcs1A transit peptide"
FT   misc_feature    2670..3181
FT                   /note="35S promoter"
FT   misc_feature    3182..3205
FT                   /note="linking region"
FT   misc_feature    3182..3185
FT                   /note="MoClo overhang in linking region"
FT   misc_feature    3202..3205
FT                   /note="MoClo overhang in linking region"
FT   misc_feature    3206..3722
FT                   /note="CSVMV promoter"
FT   misc_feature    3722
FT                   /note="MoClo overhang in CSVMV promoter"
FT   misc_feature    3723..3893
FT                   /note="Rbcs1A transit peptide"
FT   misc_feature    3723..3725
FT                   /note="MoClo overhang in Rbcs1A transit peptide"
FT   misc_feature    3890..3893
FT                   /note="MoClo overhang in Rbcs1A transit peptide"
FT   misc_feature    3894..4766
FT                   /note="mature EPYC1"
FT   misc_feature    4766
FT                   /note="MoClo overhang in mature EPYC1"
FT   misc_feature    4767..5678
FT                   /note="eGFP (with N-terminal HA-tag)"
FT   misc_feature    4767..4769
FT                   /note="MoClo overhang in eGFP (with N-terminal HA-tag)"
FT   misc_feature    5679..5932
FT                   /note="HSP terminator"
FT   misc_feature    5679..5682
FT                   /note="MoClo overhang in HSP terminator"
FT   misc_feature    5933..6199
FT                   /note="nos terminator"
FT   misc_feature    5933..5936
FT                   /note="MoClo overhang in nos terminator"
XX
SQ   Sequence 6199 BP; 1653 A; 1467 C; 1498 G; 1581 T; 0 other;
     gtcctgctga gcctcgacat gttgtcgcaa aattcgccct ggacccgccc aacgatttgt        60
     cgtcactgtc aaggtttgac ctgcacttca tttggggccc acatacacca aaaaaatgct       120
     gcataattct cggggcagca agtcggttac ccggccgccg tgctggaccg ggttgaatgg       180
     tgcccgtaac tttcggtaga gcggacggcc aatactcaac ttcaaggaat ctcacccatg       240
     cgcgccggcg gggaaccgga gttcccttca gtgagcgtta ttagttcgcc gctcggtgtg       300
     tcgtagatac tagcccctgg ggcacttttg aaatttgaat aagatttatg taatcagtct       360
     tttaggtttg accggttctg ccgctttttt taaaattgga tttgtaataa taaaacgcaa       420
     ttgtttgtta ttgtggcgct ctatcataga tgtcgctata aacctattca gcacaatata       480
     ttgttttcat tttaatattg tacatataag tagtagggta caatcagtaa attgaacgga       540
     gaatattatt cataaaaata cgatagtaac gggtgatata ttcattagaa tgaaccgaaa       600
     ccggcggtaa ggatctgagc tacacatgct caggtttttt acaacgtgca caacagaatt       660
     gaaagcaaat atcatgcgat cataggcttc tcgcatatct cattaaagca ggacaagctt       720
     atcattcctc accagcatca gcatcagggg tcttgaaagc atgttggtac tcaacgattc       780
     caagctcggt gttagagtga tcttcctcaa ctcttctgaa agcgaacata ggtccaccgt       840
     tttgaaggat agaagggtgg atagcagact tgaagtgcat gtgagaatcc accacagaag       900
     agtagtaacc accatctcta agtgagaagg ttctggtgaa agatccatcg agatcgttat       960
     ctcccatagg atgaagatgc tcaacagtag cgttagacct gatgatcttg tcggtgaaga      1020
     taacagaatc ctcagggaat ccagttccca taacctgcac atcaacaaat tttggtcata      1080
     tattagaaaa gttataaatt aaaatataca cacttataaa ctacagaaaa gcaattgcta      1140
     tatactacat tcttttattt tgaaaaaaat atttgaaata ttatattact actaattaat      1200
     gataattatt atatatatat caaaggtaga agcagaaact taccttgaaa tctccgatca      1260
     ctcttccagc ctcgtatctg taagagaagc taacgtgaag aacaccacca tcctcgtact      1320
     tctcgatcct agtgttggtg tatccaccgt tgttgatagc atgaaggaaa gggttctcgt      1380
     atccagatgg gtaagttccg aagtggtaga atccgtatcc cataacgtga gaaagaaggt      1440
     atggagagaa ggtaagagca cccttggtag acttcatctt gttagtcatt cttccctgct      1500
     caggagttcc ctcaccacct ccaacaagct cgaactcaac accgttaagg gttccagtga      1560
     ttctacactc gatttccata gcaggaagtc cagactcatc agactcagat ccagatcctc      1620
     tcgaaaggcc ctttctccag tctgcaggga gagccgttct cttaatttct ggcttgcttt      1680
     tacctgtcca aggattagtt ccggctttgt cagcggagga actagatgac gaagcgggag      1740
     cgtctctcca ggaagcggat gatgatctag caggtgcagg ggcactgctt gcaggtgcgg      1800
     gactgttgga tcggagtgac tccaactcct gtttccaatt acttggaagg gccgatctac      1860
     taggggtgac tgcttttttg ctcgccgatg agctacgagc tggggcggaa gaagcaggag      1920
     ctgcatccct ccaactagct gaggatgacc tagctggtgc tggcgcgcta gaagctggtg      1980
     ctggactgct tgatcgaagg gattcaagtt cctgtttcca gttagaaggc agagctgaac      2040
     gagacggagt gacggccttc ttgctggaag aactcctagc tggagcgcta gatgcaggcg      2100
     ctgcgtccct ccatgaggca gaactacttc tggccggagc tggcgctgat gaagccggtg      2160
     cagggctgga gcttctcaat gactccaatt cctgcttcca atttgaaggc aaggcacttc      2220
     ttgaaggagt aacggccttc tttgaagctg agctacttct ggcaggcgct gatgatgcag      2280
     gagcggcgtc tcgccacgat gcgctgctcg atctcgcagg agcaggagca gaggacgccg      2340
     ccgacgagct gccgttacca tttctgagtg attcaagttc ttgcctccaa ttagctggga      2400
     gaacgcttct tgttgggctc actctgttcg ttgcactact agctcttgag gcttgaactg      2460
     tagcagtaga tgactctctc caagatcctc ttgctgcacc tccgcagtta actcttccgc      2520
     cgttgcttgt gatggaagta atgtcgttgt tagccttgcg ggtggctggg aaggcagcgg      2580
     aggacttaag tccgttgaaa ggagcgacca tagtggcctg agccggagag gcaaccatag      2640
     tagcggaaga gagcatagag gaagccattg tatcgataat tgtaaatgta attgtaatgt      2700
     tgtttgttgt ttgttgttgt tggtaattgt tgtaaaaatg agctcttata ctcgagcgtg      2760
     tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg      2820
     attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgtagacg      2880
     tggttggaac ctcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac      2940
     cactgtcggc agagagatct tgaatgatag cctttccttt atcgcaatga tggcatttgt      3000
     aggagccacc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga      3060
     atccgaggag gtttcccgaa attatccttt gttgaaaagt ctcaatagcc ctttgatctt      3120
     ctgagactgt atctttgaca tttttggagt agaccagagt gtcgtgctcc accatgttga      3180
     ccctcgcaag aattcaagct tggagccaga aggtaattat ccaagatgta gcatcaagaa      3240
     tccaatgttt acgggaaaaa ctatggaagt attatgtaag ctcagcaaga agcagatcaa      3300
     tatgcggcac atatgcaacc tatgttcaaa aatgaagaat gtacagatac aagatcctat      3360
     actgccagaa tacgaagaag aatacgtaga aattgaaaaa gaagaaccag gcgaagaaaa      3420
     gaatcttgat gacgtaagca ctgacgacaa caatgaaaag aagaagataa ggtcggtgat      3480
     tgtgaaagag acatagagga cacatgtaag gtggaaaatg taagggcgga aagtaacctt      3540
     atcacaaagg aatcttatcc cccactactt atccttttat atttttccgt gtcatttttg      3600
     cccttgagtt ttcctatata aggaaccaag ttcggcattt gtgaaaacaa gaaaaaattt      3660
     ggtgtaagct attttctttg aagtactgag gatacaactt cagagaaatt tgtaagtttg      3720
     taatggcttc ctctatgctc tcttccgcta ctatggttgc ctctccggct caggccacta      3780
     tggtcgctcc tttcaacgga cttaagtcct ccgctgcctt cccagccacc cgcaaggcta      3840
     acaacgacat tacttccatc acaagcaacg gcggaagagt taactgcgga ggtgcagcaa      3900
     gaggatcttg gagagagtca tctactgcta cagttcaagc ctcaagagct agtagtgcaa      3960
     cgaacagagt gagcccaaca agaagcgttc tcccagctaa ttggaggcaa gaacttgaat      4020
     cactcagaaa tggtaacggc agctcgtcgg cggcgtcctc tgctcctgct cctgcgagat      4080
     cgagcagcgc atcgtggcga gacgccgctc ctgcatcatc agcgcctgcc agaagtagct      4140
     cagcttcaaa gaaggccgtt actccttcaa gaagtgcctt gccttcaaat tggaagcagg      4200
     aattggagtc attgagaagc tccagccctg caccggcttc atcagcgcca gctccggcca      4260
     gaagtagttc tgcctcatgg agggacgcag cgcctgcatc tagcgctcca gctaggagtt      4320
     cttccagcaa gaaggccgtc actccgtctc gttcagctct gccttctaac tggaaacagg      4380
     aacttgaatc ccttcgatca agcagtccag caccagcttc tagcgcgcca gcaccagcta      4440
     ggtcatcctc agctagttgg agggatgcag ctcctgcttc ttccgcccca gctcgtagct      4500
     catcggcgag caaaaaagca gtcaccccta gtagatcggc ccttccaagt aattggaaac      4560
     aggagttgga gtcactccga tccaacagtc ccgcacctgc aagcagtgcc cctgcacctg      4620
     ctagatcatc atccgcttcc tggagagacg ctcccgcttc gtcatctagt tcctccgctg      4680
     acaaagccgg aactaatcct tggacaggta aaagcaagcc agaaattaag agaacggctc      4740
     tccctgcaga ctggagaaag ggcctttcgt acccatacga tgttcctgac tatgcgggct      4800
     atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca gattacgcca      4860
     ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg      4920
     actatgcagg attgtatcca tatgacgttc cagattacgc cgtgagcaag ggcgaggagc      4980
     tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt      5040
     tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca      5100
     tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg      5160
     gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg      5220
     ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca      5280
     agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg      5340
     gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca      5400
     gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga      5460
     tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc      5520
     ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc      5580
     tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg      5640
     ccgggatcac tctcggcatg gacgagctgt acaagtaagc ttatatgaag atgaagatga      5700
     aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt      5760
     gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc tcattataat      5820
     gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct tctgcagcat      5880
     ataactactg tatgtgctat ggtatggact atggaatatg attaaagata agagatgtca      5940
     agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct      6000
     tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta      6060
     atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta      6120
     atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc      6180
     atctatgtta ctagatcga                                                   6199
//