Dbfetch

ID   HC470968; SV 1; linear; unassigned DNA; PAT; SYN; 6230 BP.
XX
AC   HC470968;
XX
DT   21-APR-2010 (Rel. 104, Created)
DT   27-APR-2010 (Rel. 104, Last updated, Version 2)
XX
DE   Sequence 4 from Patent EP2163635.
XX
KW   .
XX
OS   synthetic construct
OC   other sequences; artificial sequences.
XX
RN   [1]
RA   Song H.S., Kock M., Brown J.A., Loyall L.P., Xing L., Jia H., McMillan J.,
RA   Ireland L.;
RT   "Method for isolation of transcription termination sequences";
RL   Patent number EP2163635-A1/4, 17-MAR-2010.
RL   BASF Plant Science GmbH (DE).
XX
DR   MD5; 14f47b6306b0ba667f72e47045d6e773.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..6230
FT                   /organism="synthetic construct"
FT                   /mol_type="unassigned DNA"
FT                   /note="Nucleic acid construct
FT                   Lo522a-pENTR-C1-STPT-nptII-IRnos"
FT                   /db_xref="taxon:32630"
FT   regulatory      268..295
FT                   /note="complement: terminator"
FT                   /regulatory_class="terminator"
FT   regulatory      427..470
FT                   /note="complement: terminator"
FT                   /regulatory_class="terminator"
FT   misc_feature    591..747
FT                   /note="attR2 recombination site"
FT   regulatory      763..2081
FT                   /note="STPT promoter"
FT                   /regulatory_class="promoter"
FT   misc_feature    2112..2959
FT                   /note="nptII"
FT   regulatory      2998..3676
FT                   /note="E9 terminator"
FT                   /regulatory_class="terminator"
FT   regulatory      3682..3934
FT                   /note="complement: nos terminator"
FT                   /regulatory_class="terminator"
FT   regulatory      4063..4325
FT                   /note="nos terminator"
FT                   /regulatory_class="terminator"
FT   misc_feature    4344..4439
FT                   /note="complement: attL3 recombination site"
FT   misc_feature    4624..5433
FT                   /note="kanamycin resistance"
XX
SQ   Sequence 6230 BP; 1747 A; 1342 C; 1348 G; 1793 T; 0 other;
     ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga        60
     taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga       120
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca       180
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc       240
     tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta       300
     gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc       360
     acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa       420
     caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg       480
     gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa       540
     aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac       600
     taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg       660
     tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt       720
     ctcgttcaac tttcttgtac aaagtggggt acccggggat cctgatagct tatactcaaa       780
     ttcaacaagt tatatataaa tgtatagata ctacaatatc attaacaaaa gtcaccttaa       840
     ataaatacac atatctttta tgttctctat tgttttgcgt acgctaacac aatttctcat       900
     atgcaaaagg atgaatgagt aacaaattac ctcataagaa caatcatctt tgcttacata       960
     ctaatacaat aatcactcaa tcaaccaata acatcaatca cataggttta catacaataa      1020
     tcactcaatc aacttcataa gaagaatcat gtttacttaa ttcatcaatt atccccaaaa      1080
     acaccactat taagtataaa ctacaacata tttgtagtga tgggtcaaca tttttatcat      1140
     atttaaactc gggttccctc aaatcgagaa atagtgaaca tgtaatatta attttaaatc      1200
     gcaattacag aaattaattg aatttggtca aatggacaga attttataga ttgggtggaa      1260
     ctagaaaaaa aaaaaaaaag agtatagggt gaattgagta catgaaagta catggtaatc      1320
     ctagttaaac gcataataca tgtgggttca tttgtatttt tttgtaactt acgagtaaac      1380
     tggctacaac aaaaaaaaat tagaagattt ttttgttttg tagaaaaccc taattttagt      1440
     tatagttgta taactttgat aaaattataa aattgtatta cgaaaaaagt aataagatat      1500
     tcaaaaaagc ctagaataac gtatatgact atgagcatga aactgcaagt caaatgctga      1560
     cagacaacca taaacaaaag aaattaaata gagatacctt taaaataagt aaaatttcat      1620
     ttataaaaaa tctactttct tgtgaatctg tcacgttcaa taatttgaag accactcaac      1680
     atacaaggta aataatgaaa aataaaatct accaaaattt caatcattat tatcttccaa      1740
     aaaaacaaaa ttatacagat gatgatggtg atatggaact tcgattggct aatattcact      1800
     gtgtctctaa aaaccatcca cttatcaaga taagatggac cctacactca tccaatctaa      1860
     accagtatct caagattctt atctaattac atcattctct accgttagat gaaattgacc      1920
     attaacccta ccataactcc atacaccgcg agatactgga ttaaccaaat cgagatcatc      1980
     gtagccgtcc gatcaacaag taccatctct tgaaatactc gaaatcctca taagtccgtc      2040
     cctctttgct ctcactatca aaactctgaa tttcgatttc atctagagtc gagcccgggc      2100
     gatatcggat ctcgactcta gtcgagggcc catgggagct tggattgaac aagatggatt      2160
     gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca      2220
     gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct      2280
     ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct      2340
     atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc      2400
     gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct      2460
     tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga      2520
     tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg      2580
     gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc      2640
     agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac      2700
     ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat      2760
     cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga      2820
     tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc      2880
     cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg      2940
     acccaagcta gcttcgacgg atcctctagc atatgctcga ggcggccgca gatatcagat      3000
     cctctagcta gagctttcgt tcgtatcatc ggtttcgaca acgttcgtca agttcaatgc      3060
     atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg gcattgggaa      3120
     aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt      3180
     cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt      3240
     tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga      3300
     aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct      3360
     aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat gcttattcac      3420
     taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa tacaagtatg      3480
     tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag aatccttgtc      3540
     agattctaat cattgcttta taattatagt tatactcatg gatttgtagt tgagtatgaa      3600
     aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat caatcgacct      3660
     gcagcccaag cttgatctag tccgatctag taacatagat gacaccgcgc gcgataattt      3720
     atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac      3780
     tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg      3840
     cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa      3900
     tcttaagaaa ctttattgcc aaatgtttga acgatcgggg aaattcgagc tcggtagcaa      3960
     ttcccgaggc tgtagccgac gatggtgcgc caggagagtt gttgattcat tgtttgcctc      4020
     cctgctgcgg tttttcaccg aagttcatgc cagtccagcg tgtcgaccga tcggtcaaac      4080
     atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata      4140
     taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt      4200
     atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac      4260
     aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat      4320
     cgggaagctt ggcgtaatca tggcaacttt attatacaaa gttggcatta taaaaaagca      4380
     ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg      4440
     gagctccatg gtagcgttaa cgcggccatc ccctatagtg agtcgtatta catggtcata      4500
     gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat      4560
     aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt      4620
     gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct      4680
     gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat      4740
     cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt      4800
     gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt      4860
     ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc      4920
     cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt      4980
     gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt      5040
     aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt      5100
     gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa      5160
     atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt      5220
     gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga      5280
     atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct      5340
     tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg      5400
     cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac      5460
     tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac      5520
     gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct      5580
     tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca      5640
     gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc      5700
     agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc      5760
     aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct      5820
     gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag      5880
     gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc      5940
     tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg      6000
     agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag      6060
     cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt      6120
     gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac      6180
     gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt                 6230
//