Dbfetch
ID GZ298614; SV 1; linear; genomic DNA; PAT; UNC; 7370 BP. XX AC GZ298614; XX DT 06-JUN-2012 (Rel. 113, Created) DT 06-JUN-2012 (Rel. 113, Last updated, Version 1) XX DE Sequence 163 from patent US 8173410. XX KW . XX OS unidentified OC unclassified sequences. XX RN [1] RP 1-7370 RA Bott R.R., Cervin M.A., Kellis J.T.Jr., McAuliffe J.C., Miasnikov A., RA Peres C.M., Rife C.L., Wells D.H., Weyler W., Whited G.M.; RT "Isoprene synthase variants for improved microbial production of isoprene"; RL Patent number US8173410-B2/163, 08-MAY-2012. RL Danisco US Inc.; RL Palo Alto, CA. XX DR MD5; 7f2fd76957e858f6f64640bb48953eb0. XX FH Key Location/Qualifiers FH FT source 1..7370 FT /organism="unidentified" FT /mol_type="genomic DNA" FT /db_xref="taxon:32644" XX SQ Sequence 7370 BP; 1713 A; 1973 C; 2015 G; 1669 T; 0 other; aagggcgagc tcaacgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct 60 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggagt 120 tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca 180 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat 240 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc 300 attaaagctt atcgatgata agctgtcaaa catgagaatt aattcttgaa gacgaaaggg 360 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc 420 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 480 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 540 aaggaagagt atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga 600 gaggctattc ggctatgact gggcacaact gacaatcggc tgctctgatg ccgccgtgtt 660 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct 720 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg 780 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt 840 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc 900 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc 960 gaaacatcgc atcgagcggg cacgtactcg gatggaagcc ggtcttgtcg atcaggatga 1020 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg 1080 catgcccgac ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat 1140 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg 1200 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc 1260 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta 1320 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg 1380 acgcctaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1440 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1500 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1560 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1620 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1680 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1740 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1800 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1860 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1920 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 1980 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2040 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2100 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2160 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2220 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2280 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 2340 ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac 2400 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg 2460 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 2520 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 2580 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg 2640 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga 2700 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg 2760 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa 2820 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt 2880 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt 2940 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct 3000 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac 3060 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag 3120 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc 3180 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac 3240 ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg 3300 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt 3360 cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc 3420 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa 3480 tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg 3540 gtccaatgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat 3600 ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg 3660 aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga 3720 cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt 3780 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa 3840 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga 3900 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga 3960 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca 4020 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac 4080 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 4140 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg 4200 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg 4260 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat 4320 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg 4380 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca 4440 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc 4500 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag 4560 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc 4620 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa 4680 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg 4740 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca 4800 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt 4860 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg 4920 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac 4980 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc 5040 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa 5100 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca 5160 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg 5220 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa 5280 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa 5340 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 5400 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 5460 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 5520 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat 5580 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgcggg 5640 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc 5700 gggatctgta cgacgatgac gataaggatc atcccttcac catgcgtcgt tctgcgaact 5760 acgaacctaa cagctgggac tatgattacc tgctgtcctc cgacacggac gagtccatcg 5820 aagtatacaa agacaaagcg aaaaagctgg aagccgaagt tcgtcgcgag attaataacg 5880 aaaaagcaga atttctgacc ctgctggaac tgattgacaa cgtccagcgc ctgggcctgg 5940 gttaccgttt cgagtctgat atccgtggtg cgctggatcg cttcgtttcc tccggcggct 6000 tcgatgcggt aaccaagact tccctgcacg gtacggcact gtctttccgt ctgctgcgtc 6060 aacacggttt tgaggtttct caggaagcgt tcagcggctt caaagaccaa aacggcaact 6120 tcctggagaa cctgaaggaa gatatcaaag ctatcctgag cctgtacgag gccagcttcc 6180 tggctctgga aggcgaaaac atcctggacg aggcgaaggt tttcgcaatc tctcatctga 6240 aagaactgtc tgaagaaaag atcggtaaag agctggcaga acaggtgaac catgcactgg 6300 aactgccact gcatcgccgt actcagcgtc tggaagcagt atggtctatc gaggcctacc 6360 gtaaaaagga ggacgcgaat caggttctgc tggagctggc aattctggat tacaacatga 6420 tccagtctgt ataccagcgt gatctgcgtg aaacgtcccg ttggtggcgt cgtgtgggtc 6480 tggcgaccaa actgcacttt gctcgtgacc gcctgattga gagcttctac tgggccgtgg 6540 gtgtagcatt cgaaccgcaa tactccgact gccgtaactc cgtcgcaaaa atgttttctt 6600 tcgtaaccat tatcgacgat atctacgatg tatacggcac cctggacgaa ctggagctgt 6660 ttactgatgc agttgagcgt tgggacgtaa acgccatcaa cgacctgccg gattacatga 6720 aactgtgctt tctggctctg tataacacta ttaacgaaat cgcctacgac aacctgaaag 6780 ataaaggtga gaacatcctg ccgtatctga ccaaagcctg ggctgacctg tgcaacgctt 6840 tcctgcaaga agccaagtgg ctgtacaaca aatctactcc gacctttgac gactacttcg 6900 gcaacgcatg gaaatcctct tctggcccgc tgcaactggt gttcgcttac ttcgctgtcg 6960 tgcagaacat taaaaaggaa gagatcgaaa acctgcaaaa ataccatgac accatctctc 7020 gtccttccca tatcttccgt ctgtgcaatg acctggctag cgcgtctgcg gaaattgcgc 7080 gtggtgaaac cgcaaatagc gtttcttgtt acatgcgcac taaaggtatc tccgaagaac 7140 tggctaccga aagcgtgatg aatctgatcg atgaaacctg gaaaaagatg aacaaggaaa 7200 aactgggtgg tagcctgttc gcgaaaccgt tcgtggaaac cgcgatcaac ctggcacgtc 7260 aatctcactg cacttatcat aacggcgacg cgcatacctc tccggatgag ctgacccgca 7320 aacgcgttct gtctgtaatc actgaaccga ttctgccgtt tgaacgctaa 7370 //