ID AB326225; SV 1; linear; genomic RNA; STD; VRL; 7561 BP. XX AC AB326225; XX DT 19-JUN-2007 (Rel. 92, Created) DT 17-AUG-2007 (Rel. 92, Last updated, Version 3) XX DE Apple chlorotic leaf spot virus genomic RNA, complete genome, strain: MO-5. XX KW . XX OS Apple chlorotic leaf spot virus OC Viruses; Riboviria; Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus. XX RN [1] RP 1-7561 RA Yoshikawa N., Yaegashi H.; RT ; RL Submitted (15-JUN-2007) to the INSDC. RL Nobuyuki Yoshikawa, Iwate University, Faculty of Agriculture; Ueda 3-18-8, RL Morioka, Iwate 020-8550, Japan (E-mail:yoshikawa@iwate-u.ac.jp, RL Tel:81-19-621-6150, Fax:81-19-621-6150) XX RN [2] RX DOI; 10.1099/vir.0.82984-0. RX PUBMED; 17698674. RA Yaegashi H., Isogai M., Tajima H., Sano T., Yoshikawa N.; RT "Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in RT the coat protein of apple chlorotic leaf spot virus are crucial for RT infectivity"; RL J. Gen. Virol. 88(Pt 9):2611-2618(2007). XX DR MD5; 41942608363248158228af9aa89fb365. DR EuropePMC; PMC4982687; 27519059. XX FH Key Location/Qualifiers FH FT source 1..7561 FT /organism="Apple chlorotic leaf spot virus" FT /sub_species="Plant virus" FT /strain="MO-5" FT /mol_type="genomic RNA" FT /db_xref="taxon:12175" FT CDS 153..5786 FT /codon_start=1 FT /transl_table=1 FT /gene="POL" FT /product="RNA polymerase" FT /db_xref="GOA:A6BMF2" FT /db_xref="InterPro:IPR001788" FT /db_xref="InterPro:IPR002588" FT /db_xref="InterPro:IPR005123" FT /db_xref="InterPro:IPR007094" FT /db_xref="InterPro:IPR008744" FT /db_xref="InterPro:IPR027351" FT /db_xref="InterPro:IPR027417" FT /db_xref="InterPro:IPR037151" FT /db_xref="UniProtKB/TrEMBL:A6BMF2" FT /protein_id="BAF64465.1" FT /translation="MAFSYRTPQEELLSRLPQSQQEVISGYQYERIQKEEEKKVENFSF FT YLPERTKEWFTKSGVYLSPFAYVNHSHPGCKTLENHLLFNVVASYISKYPYVACLSIKS FT NKMSKMERLGPNSVRTYDILNRLVTSKDKARYGPLATPERSPCPKKTNIFIHDEIHYWS FT RTQLESFLMVHRPKNLWATLVFPPEILAGYKSSVLPFLYQFEIHGKDLVYMPDGVRSES FT YTQPLENGYLLSSSSILVHNKKTGVELRYQVSLVYSLGSHHLFHIFPTEDLLKEEVRRF FT GPYDLFDVGSLFVKPVRVPIQDFPLSVFKKIFIYLSSLKKPDVQSAVAKLRQLSDADIS FT IESVFMVQEFASRIEKNGVGSWSCSFWECMKDWFFDKLPYREVIEKVGLADDFTRRLMK FT LKPLAFDIHTSDRPLTVRMVIDSIWEERVCMEDSLPNQIFYGRKEWIQYGVMPMIKRGF FT SKLVPGRDMLEPSYPQEIFSDLLSSTPLGKSYDSRLHSKIASPMVVMRSSHGYSEAAEF FT KSNCLSICASDDSNKAEKTAYEKEFIEKRRSISNKCLDFHIRKAKVKSLSELAIRLSRV FT DKAPSVRRKRKVNVRLVPSYLTCSSDVKEVKGNDKLASTVHSENEGNTQGNETQVALDR FT KKTKVSEGSGPEGKKEEGWFSLSHLTGKEEICSAGLIRTVGNDFLHLERQIEDLPLVQI FT GRRKAAYFCLDFPLVYFHDKVSYPTFEATGPIKQAIINAKNKWGIDFNSALVQVYEDGC FT NLPLHKDNEECYDDDGILTINVCGDAKFHTTCHDEIIDLKQGNEILMPAGFQKRNKHAV FT QVISEGRTSVTMRVHKRDFSFESKVKFVKGRLDCLFSSIAEVIHKKPEEVMAFVPHILD FT RCISNKGCSIDDARAICEAYEIKIECEGDCGLVECGSVGLSVGRLLLRGNHFRVASVRR FT SSIDSLANSSKEIKANGVLDHVMFNFHDRLKAVEPDLNSVEVKVNLERAGKLLKSLMDG FT MTGIVSHNSTHEGWRMIKGINSTSEMRSFMKMIKDDDSNEKNEVSEKLQGLNFFKKKIY FT GIFGFAGSGKSHAIQNLIHSEFKNSQGILVVCPRKFLAKDWSEKGVNEKDIKTFESALK FT SDIKGKRLFILDEVSLLPKGFTDLLMLKLHMDGVLKVSTIVCIGDPLQAGYFCPKDDGY FT LSKDGEITRLFKEGVKYKWYSYRINRFMAKKLGISTMNDFTGIDQQSLIYKDMPSAQHF FT LREGNNHPEVILVASMIEKELYSNYGNVMTFGESQGLTFNCGVIVLSEEAKLCSDAHVM FT VAITRFRRGFCFALGSKGSKEDYMRSMKSGLLQRMCSGIGASKEFILNSSSVKLVLSEK FT DVAKGAGIDEMDREVRLEGDVWLKSMIYLGKRYHLVEPLGQVIKLTESAIKCHIPVCSH FT QTLMPELEKIQAREYREFKGRDGWSNQFREEAGPNWKFPYKVNQAMNFEAVYPRHKMDD FT DLTFLAAIKKRLRFDNVANNYAKLKASENRGKYLAKVFLKHVPIKCGRDQRLLDQCRQE FT FEETKLSKSAATIGAHAQRSDSDWPLDKIFLFMKSQLCTKFEKRFTEAKAGQTLACFPH FT KILVEFSPWCRYTEKVLTANLPDNYYIHQRKNFCELEDFARRFSNGSMCVESDYTAFDV FT SQDHTILAFEVELLRHFGWDENILQSYIRMKCTLGCRLGGFAIMRFTGEFSTFLFNTLA FT NMVFTFCRYEVPDGTPICFAGDDMCALRNLREIDTHNFILSKLSLKAKVNRTKVPMFCG FT WRLCCDGLIKEPCLIYERLQVAIENGRLMDVIDSYFLEFSFAYKLGERLYSHLEIEQLN FT YHQVLTRFFIRNKHLLRGDSRHNISELEWLSDEDGDDDKGSQVEDRRRGYSNCWGEKLQ FT NLF" FT CDS 5704..7080 FT /codon_start=1 FT /transl_table=1 FT /gene="MP" FT /product="movement protein" FT /db_xref="GOA:A6BMF3" FT /db_xref="InterPro:IPR001815" FT /db_xref="InterPro:IPR028919" FT /db_xref="UniProtKB/TrEMBL:A6BMF3" FT /protein_id="BAF64466.1" FT /translation="MMIRGHKLKIAEGDIPIAGVKSSRIYSDVSPFKRASDLMIHWNEF FT VFKVMPEDIAGDGFRLASIPVIPDSEVKAVIRKRDSTNYVHWGALSISIDALFKKNAGV FT SGWCYVYDNRWETFEQAKLQKFRFNLDSGSATLVTSPNFPVSLDDPGLSNSICVAVMFE FT NLNFKLDSYPISVRVGTMCRFFDSFLSNVKNKNDSNFLLEASNADPLGASVFGFDDDDQ FT VSELFNYIQTVPTQAIKSREHEIPRGMFGIMGKKTVKSFEFASGSKGLNKIKPQRGRSS FT GRSSSQRFAPGFKSQDEKVEHQGLATDLDFENFLKDEGRRKAGSKSIASEGSSVDNISS FT REFQFARQNQAKENGGSSKSSTKGGRRSESVPGRRRQTPSWKDRGNSGTDTGVHLREHS FT DPGNLGADGVPGLGGGGQVNGGSEGGEFLQSEVSGGSHQDLQNYIFGPEHKRDDFPSGL FT " FT CDS 6764..7345 FT /codon_start=1 FT /transl_table=1 FT /gene="CP" FT /product="coat protein" FT /db_xref="GOA:A6BMF4" FT /db_xref="InterPro:IPR008879" FT /db_xref="UniProtKB/TrEMBL:A6BMF4" FT /protein_id="BAF64467.1" FT /translation="MAAVLNLQLKVDADLKVFLAAEGRPLHGKTGAILELTLESIFANI FT AIQGTSEQTEFLDLVVEVKSMEDQKVVNSYNLKSVVDLIKIFKTTSSDPNINGMTFRQV FT CEAFAPEARDGLVKLKYKGVFTNLFTTMPEVGGKYPELMFDFNKGLNMFIMNKAQQKVI FT TNMNRRLLQTEFAKSENEAKLSSVTTDLCI" XX SQ Sequence 7561 BP; 2292 A; 1359 C; 1842 G; 2068 T; 0 other; tgatactgaa gaagtgtaca ctcacgtcgt gagtagacaa gtcgacgtaa cgtctcgact 60 gtggccaagg gtcaacatct ttatgatgtt ataaaaacaa atacaatcac attgtattcc 120 aaagctttcc gtgtgagcac gtgagagatt aaatggcttt ctcgtacaga acgccacaag 180 aggagctcct aagtagattg ccacaatctc agcaggaggt tatcagcggc taccaatatg 240 agcgcattca aaaggaggaa gagaagaagg tggaaaactt ctctttttac ctaccggaga 300 gaaccaagga atggttcact aagtctggtg tctatttgtc acctttcgct tatgtgaatc 360 actctcaccc tggttgcaaa actctggaga accacctttt atttaatgtt gtagctagct 420 acattagtaa gtatccatat gtagcttgtt taagtattaa atctaacaaa atgagcaaaa 480 tggaacgttt aggacccaac tctgttcgta cgtatgacat cctgaatagg ctcgttacct 540 ctaaggataa agcaagatat ggaccattgg ccactcccga gagatcacca tgtccaaaaa 600 aaaccaatat tttcatacat gatgaaatcc actattggtc cagaacccag cttgagagtt 660 tccttatggt gcacagacca aagaatttat gggccactct tgtctttcct cctgaaattt 720 tagctggtta caaatcttct gtacttcctt tcctatacca atttgaaatt catggaaaag 780 atcttgtgta catgcctgat ggggttcggt ctgagagtta cacgcaacct cttgagaatg 840 ggtatctgct cagctccagt agtattttgg ttcataacaa aaaaactggt gttgagttac 900 gttatcaggt tagcctggtt tattcccttg gctctcatca tttattccat attttcccaa 960 ctgaggacct acttaaggag gaagtgagaa gatttgggcc atatgacctt tttgacgttg 1020 gtagtttatt cgtcaaacca gttagggtgc ccattcagga ttttccactc agcgtgttca 1080 aaaaaatttt tatctatctg agttccttaa aaaaacctga tgttcaatct gcggtggcaa 1140 aactgagaca gctgtcagac gcggacattt ccatagagtc cgtgtttatg gttcaagaat 1200 tcgcaagcag aatagaaaag aatggggttg gaagttggtc atgctccttc tgggagtgca 1260 tgaaggattg gttctttgac aagctccctt atagagaagt cattgaaaag gttggtttgg 1320 ctgatgactt cacgagacgt ctaatgaaac ttaagccgct ggcttttgat attcacacct 1380 cagatagacc attgacagtg aggatggtaa ttgacagcat atgggaggaa agagtctgca 1440 tggaagatag cttgcccaac caaatcttct acgggcgtaa ggagtggata caatatgggg 1500 ttatgcccat gataaagagg ggtttttcaa aacttgtgcc aggtcgtgac atgcttgaac 1560 cctcatatcc tcaggagatt ttctcagacc tactttcttc aactccttta ggcaaatctt 1620 atgactccag gctgcattcg aagatcgcaa gcccaatggt cgtgatgcgt tcttcacacg 1680 gttactcaga ggccgcggaa tttaaatcga attgccttag tatatgtgcg tcagatgatt 1740 caaataaggc agagaaaacc gcatacgaga aggagtttat tgagaaaagg agaagcattt 1800 ccaacaaatg tttggatttt cacatcagga aagcaaaggt taaaagtttg agtgagcttg 1860 caatcagact ttcaagggta gacaaggctc cttccgtcag gagaaagagg aaggtcaacg 1920 tccgtctggt tcctagttac ttaacatgct catctgatgt gaaggaagtc aaagggaatg 1980 ataagctcgc ttcgactgtg cactctgaga atgaagggaa cactcaagga aatgagacac 2040 aagttgccct cgataggaag aaaactaaag tcagtgaagg ttcaggtcca gagggaaaga 2100 aagaagaagg ctggttttca ttgtcgcatt taactggaaa ggaggagatt tgttctgctg 2160 ggctcattag gaccgtgggt aatgattttc tccatcttga gagacaaata gaggatctgc 2220 ctcttgtgca aataggaaga cgaaaggccg cctacttctg tttggacttt cctttggtgt 2280 acttccacga caaggtgtca taccctactt tcgaggcaac agggccaatc aagcaggcga 2340 taatcaacgc aaagaataaa tggggaattg acttcaacag tgctcttgtt caagtttacg 2400 aagatggctg caatcttcct ttgcacaaag acaatgagga gtgttatgat gacgatggta 2460 tactcactat taatgtctgt ggagatgcta agttccacac gacctgtcat gatgagatta 2520 ttgacctgaa gcaaggaaat gaaatcctga tgcctgctgg ttttcaaaag agaaacaagc 2580 acgctgttca agtgatctcg gagggtagaa caagtgtgac aatgagagtc cacaaacgtg 2640 actttagttt cgaatccaaa gtcaaatttg tcaaagggag attagactgc cttttctctt 2700 caattgccga agtgatccac aagaaaccag aggaggtgat ggctttcgtt ccccacatcc 2760 tggataggtg catcagcaat aaaggctgtt cgattgatga tgcgagggcg atatgcgagg 2820 cctatgagat aaagattgag tgtgaagggg actgcggttt ggtggaatgt ggaagtgttg 2880 gactgtctgt agggaggctc ttgctgagag gtaatcattt tcgagtcgca tcagtaagga 2940 gatcatcaat tgactccctt gctaactcta gcaaggaaat caaggccaat ggagttcttg 3000 accatgtcat gtttaatttt catgacagac ttaaggcagt tgaaccagat ctgaactctg 3060 ttgaggttaa ggtcaatctt gagcgtgccg gtaagttgct caaaagttta atggatggaa 3120 tgacggggat agtgtcacac aattcaactc atgaagggtg gaggatgatc aaagggataa 3180 acagcacatc tgagatgaga agtttcatga agatgattaa ggacgatgat tccaatgaaa 3240 aaaatgaagt ctctgagaag ctccaaggtc tgaatttttt taaaaagaaa atctacggaa 3300 tctttggttt tgctggttcg ggcaaaagtc atgcaatcca aaatttaatt cattctgaat 3360 tcaagaactc acaaggtatt ttggttgtat gtccaaggaa atttctcgcc aaggattgga 3420 gtgaaaaggg tgtgaatgag aaggatatca aaacttttga gagtgctctt aaaagtgaca 3480 tcaaaggaaa aagacttttc attcttgatg aggtaagttt gctacctaag gggtttacag 3540 atcttctgat gctcaagtta catatggatg gagttctcaa ggtctctaca attgtgtgca 3600 taggggatcc actgcaggct gggtacttct gcccaaagga cgatgggtat ctatcaaaag 3660 atggcgagat aacgagactt ttcaaggagg gagtcaagta caagtggtac agttacagaa 3720 tcaatagatt catggcaaag aagttgggga taagcactat gaatgatttc acggggattg 3780 atcaacagag tttgatttac aaggacatgc catctgctca gcattttcta agggaaggaa 3840 ataatcaccc tgaagtgatc ctggttgcca gcatgataga gaaagagctc tattctaatt 3900 atggaaatgt gatgaccttt ggggaatctc aaggcctaac tttcaactgc ggtgtcattg 3960 tgctttctga agaagctaaa ctgtgctccg atgcacatgt catggttgcc ataacaagat 4020 tcagaagagg tttctgtttc gctcttggaa gcaaggggtc aaaagaggat tacatgaggt 4080 caatgaaaag tggtcttctg caaagaatgt gctcggggat aggagcttcc aaagagttta 4140 tcctgaactc atcatcagtt aaacttgtct tgagtgagaa ggatgtggcg aaaggagcag 4200 gaatagatga gatggacaga gaggtcagat tagaagggga tgtttggctt aaaagcatga 4260 tctaccttgg gaaaagatac catcttgttg aaccattggg tcaggtgatc aagttaactg 4320 agagtgcaat caaatgtcat atcccagtat gctcccacca gactttgatg ccggaattgg 4380 aaaaaattca ggcaagagag tacagagaat tcaaagggag ggatggttgg tcaaaccaat 4440 tcagagagga agcggggcca aattggaaat ttccttacaa agttaatcag gccatgaatt 4500 ttgaggcagt gtatccaagg cacaaaatgg atgatgatct cacttttctg gctgccatta 4560 agaagagatt acggtttgac aatgttgcca acaattatgc aaaactcaag gcatcagaaa 4620 atagaggcaa atatctagca aaagtttttc tcaagcatgt tccaatcaaa tgcggaagag 4680 accaaagatt acttgatcag tgcagacaag agtttgagga aaccaagctt tcaaagagcg 4740 cagccacaat aggtgcacat gctcagaggt cagattcgga ttggccactg gacaaaatat 4800 ttctctttat gaaatcccaa ttgtgtacaa agttcgaaaa gagattcaca gaggccaagg 4860 ccggacaaac acttgcatgt tttccacata aaattctggt cgaatttagt ccctggtgca 4920 gatacacaga aaaggtcctt acagcgaatc tgcccgataa ttactacatt catcagagaa 4980 agaacttttg tgagttggag gattttgcaa ggagattttc aaatggctcc atgtgcgtcg 5040 aatcggatta caccgctttt gatgtttctc aagaccatac aatacttgca tttgaggtgg 5100 aacttctgag gcattttggc tgggatgaaa acattcttca aagttacatc aggatgaagt 5160 gcaccttagg gtgcagattg ggcggttttg caataatgag attcacagga gaattttcaa 5220 ctttcttgtt caatactttg gcgaacatgg tgttcacttt ttgcaggtat gaagttccag 5280 atggcacacc aatatgtttt gctggggatg atatgtgtgc tctgaggaat ttgagagaaa 5340 tagataccca taactttatt ttgagcaaat tgagccttaa agcgaaagtg aacaggacaa 5400 aggtgccaat gttttgtggg tggagacttt gctgtgatgg attgattaag gaaccatgct 5460 taatttatga gaggttgcaa gttgccatag aaaatgggag attgatggat gtgatagatt 5520 cctatttctt ggagttttca tttgcctaca agttaggtga gaggctctat tcacatcttg 5580 aaattgaaca gttgaattat caccaggttc taacaaggtt cttcatcagg aacaagcatt 5640 tgctaagggg tgattcgagg cataatattt cggaattgga gtggctttca gacgaggatg 5700 gcgatgatga taaggggtca caagttgaag atcgcagaag gggatattcc aattgctggg 5760 gtgaaaagct ccagaatcta ttctgatgtg agtcctttca aaagggcctc tgacctcatg 5820 atccattgga atgagtttgt atttaaggtc atgccggagg acatagcagg tgatggcttc 5880 agactagctt cgattccagt catacctgac tctgaggtga aggcggtgat taggaaaaga 5940 gatagcacca attatgttca ttggggagcg ctttcaattt caatagacgc attgtttaag 6000 aagaatgctg gtgtctccgg atggtgttat gtgtatgaca acaggtggga aacctttgaa 6060 caagccaagc tgcagaagtt taggtttaac cttgatagtg gatcagccac gctggtgact 6120 tctccaaatt tccctgtttc tcttgatgat ccgggacttt caaattcaat ctgtgtcgcg 6180 gtgatgtttg agaatctaaa cttcaagttg gatagctacc ctatcagcgt tagggtcggg 6240 accatgtgca gattcttcga cagcttcttg agcaatgtga agaacaaaaa tgattcaaac 6300 ttccttttgg aagcttcaaa tgcagaccca ctaggtgcaa gcgtctttgg gtttgatgac 6360 gatgatcagg tgagtgagct gtttaactac atccaaaccg tgcctaccca agcgatcaaa 6420 tcaagggagc acgaaatccc aagaggaatg tttgggatta tggggaagaa aactgtcaaa 6480 tcatttgaat tcgcatctgg gtcaaagggc ttaaacaaga taaagccaca gaggggaaga 6540 tcgtcaggga gatcatcaag ccaaagattt gcccctggat ttaagtccca ggatgagaaa 6600 gttgaacatc aaggcttggc aactgatttg gattttgaaa actttctgaa agacgaaggg 6660 agaaggaaag ctggatcaaa atccattgct tcagaagggt catccgtgga taacatttct 6720 tcaagagaat ttcagttcgc tagacaaaat caggcgaagg agaatggcgg cagttctaaa 6780 tcttcaacta aaggtggacg cagatctgaa agtgttcctg gccgcagaag gcagacccct 6840 tcatggaaag acaggggcaa ttctggaact gacactggag tccatcttcg cgaacatagc 6900 gatccaggga acctcggagc agacggagtt cctggacttg gtggtggagg tcaagtcaat 6960 ggaggatcag aaggtggtga attcctacaa tctgaagtca gtggtggatc tcatcaagat 7020 cttcaaaact acatcttcgg acccgaacat aaacgggatg actttccgtc aggtctgtga 7080 ggcttttgct ccggaggcaa gagacgggct ggtcaaacta aagtacaaag gggtcttcac 7140 aaacctcttt acaaccatgc ctgaagtggg tggcaaatac ccagagctca tgttcgactt 7200 caacaaaggt ttgaatatgt ttataatgaa caaagctcag caaaaggtaa ttactaatat 7260 gaaccggcgt cttttacaga ctgagtttgc aaagagtgaa aacgaggcga aactttcgtc 7320 tgtcacgact gatctttgca tttaatctgt tgaagaggct tggttctata aataaaatga 7380 ataaataaac agtgtgtgtt tgatatgtat aattgtatta gcagttattt gcttaaataa 7440 tttgtgtatg tcgttgcatt cgaactcttg aacccatgaa agagtataaa gagtcatggt 7500 gttaatttgg agtgtttaga cttataataa atagcctgta gacttttaaa tattttacta 7560 c 7561 //