ID   L38899; SV 1; linear; genomic RNA; STD; VRL; 1132 BP.
XX
AC   L38899;
XX
DT   26-JAN-1996 (Rel. 46, Created)
DT   29-MAY-2003 (Rel. 75, Last updated, Version 3)
XX
DE   Rice ragged stunt virus (isolate from Thailand) segment S9 38 kDa spike
DE   protein gene, complete cds.
XX
KW   spike protein; structural protein; RT-PCR identification;
KW   reverse transcription-PCR identification.
XX
OS   Rice ragged stunt virus
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Spinareoviridae; Oryzavirus.
XX
RN   [1]
RP   1-1132
RX   DOI; 10.1007/BF01322684.
RX   PUBMED; 7503693.
RA   Upadhyaya N.M., Yang M., Kositratana W., Ghosh A., Waterhouse P.M.;
RT   "Molecular analysis of rice ragged stunt oryzavirus segment 9 and sequence
RT   conservation among isolates from Thailand and India";
RL   Arch. Virol. 140(11):1945-1956(1995).
XX
DR   MD5; b29d8fd8beee1879aa1bdb9db94d90b1.
XX
CC   Original source text: Rice ragged stunt virus (individual_isolate
CC   Thailand) (clone: CS976) cDNA to genomic RNA; Rice ragged stunt
CC   virus (individual_isolate Thailand) (clone: RR053) cDNA to genomic
CC   RNA; Rice ragged stunt virus (individual_isolate Thailand) (clone:
CC   CS974) cDNA to genomic RNA; Rice ragged stunt virus
CC   (individual_isolate Thailand) (clone: RR479) cDNA to genomic RNA;
CC   and Rice ragged stunt virus (individual_isolate Thailand) (clone:
CC   CS975) cDNA to genomic RNA.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1132
FT                   /organism="Rice ragged stunt virus"
FT                   /isolate="Thailand"
FT                   /mol_type="genomic RNA"
FT                   /clone="CS976"
FT                   /clone="CS974"
FT                   /clone="CS975"
FT                   /note="RT-PCR cloning was used"
FT                   /db_xref="taxon:42475"
FT   source          11..457
FT                   /organism="Rice ragged stunt virus"
FT                   /isolate="Thailand"
FT                   /mol_type="genomic RNA"
FT                   /clone="RR053"
FT                   /note="RT-PCR cloning was used"
FT                   /db_xref="taxon:42475"
FT   source          167..731
FT                   /organism="Rice ragged stunt virus"
FT                   /isolate="Thailand"
FT                   /mol_type="genomic RNA"
FT                   /clone="RR479"
FT                   /note="RT-PCR cloning was used"
FT                   /db_xref="taxon:42475"
FT   CDS             14..1030
FT                   /codon_start=1
FT                   /standard_name="structural polypeptide"
FT                   /product="spike protein"
FT                   /note="Confirmed by the presence of a 38 kDa immunoreactive
FT                   (to IgG against virus and S9 specific fusion protein)
FT                   polypeptide with N-terminal amino acid sequence identical
FT                   to that inferred from the ORF"
FT                   /db_xref="UniProtKB/TrEMBL:Q76VJ7"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAA85465.1"
FT                   /translation="MKTAFARDPFTAPATGTYGTIYASRSLPRLSISKFLEDANPEIYE
FT                   LSRYEALGTNRPSSGKRAMQPAVSKPALLETVFTLDIWYRRTNNQNIGNLRDSVSRFLS
FT                   DDRVREAVMVRLDLDIVVQLKEYWLIVKDKEAQTFADRLAFDSHLFVNRGENANYDLVT
FT                   QTFIPSDAFLKDNFKTEALKKLLLSVQNHTGLDAGLQGDSSKATYNIGLGQYLEDEALL
FT                   YRQGVALQQMAFAELELARGAEKEAFPSTFDLSNRPACNLILKRTCKWYQQTFKAEERK
FT                   EFAKSLWVDDFAEANWNTGNLSFGFSTTLNVIERWRLTRFYVHMYSSVHIYSQKASG"
XX
SQ   Sequence 1132 BP; 331 A; 237 C; 275 G; 289 T; 0 other;
     gataaatctc gccatgaaga ctgcctttgc cagagatcct tttacagctc ctgctacagg        60
     tacgtacgga accatatatg cctccagatc attaccccga ctctctatct ctaagttctt       120
     ggaagatgcg aatccagaaa tttatgaact atcgagatat gaagcgctcg gaaccaacag       180
     acccagctca gggaagcgag caatgcaacc agccgtcagt aaaccagctt tactggaaac       240
     cgtattcaca ctcgatatat ggtacagaag gacgaacaac cagaacatcg gcaatttaag       300
     agattcggta tctcgatttc tatctgatga tcgagtaaga gaagcggtga tggtgcgact       360
     agatttagac attgttgtcc agctaaaaga atactggttg atagttaaag acaaagaagc       420
     ccagactttc gcggaccgat tggcctttga ctcgcatcta tttgtcaatc gcggcgaaaa       480
     cgcgaattat gatcttgtaa ctcagacgtt cattccgagt gatgcttttc ttaaggataa       540
     ctttaagacg gaagcactta agaagttgtt gctaagtgtc caaaaccata cgggactaga       600
     cgctggattg caaggtgata gttcgaaagc gacttacaac attggcctag gacaatatct       660
     cgaggatgag gccctcctgt accgtcaagg tgtggctcta caacaaatgg cttttgcgga       720
     gttggaatta gcgagaggag cagagaaaga ggcgtttcca tcaaccttcg atctaagcaa       780
     tagaccggct tgcaacttga tccttaagcg aacgtgtaag tggtatcaac aaacgtttaa       840
     ggctgaggag agaaaggaat ttgcgaagag tttgtgggtg gatgactttg ccgaggctaa       900
     ttggaacaca ggtaatttgt cttttggctt ttcaaccaca ttaaatgtaa ttgagagatg       960
     gcgtttgacc agattttatg ttcatatgta ctcatctgtg catatatatt ctcagaaggc      1020
     ctcggggtag gaaacgctgg ggatcaaggt gaattgaaat gaggacttct caccctcatg      1080
     gacattcggc ctcttcacgg gtggcacccc gaaaactgcg agaccatggt gc              1132
//