Dbfetch
ID JX513897; SV 1; linear; genomic RNA; STD; VRL; 7601 BP. XX AC JX513897; XX DT 28-OCT-2012 (Rel. 114, Created) DT 28-OCT-2012 (Rel. 114, Last updated, Version 1) XX DE Grapevine virus B isolate GVB 3138-01 replicase (ORF1), 20 kDa protein DE (ORF2), movement protein (ORF3), coat protein (CP), and RNA-binding protein DE (ORF5) genes, complete cds. XX KW . XX OS Grapevine virus B OC Viruses; Riboviria; Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus. XX RN [1] RP 1-7601 RA Rott M.E., Belton M., Saeed H.; RT "Detection of grapevine and tree fruit viruses by next generation RT sequencing"; RL Unpublished. XX RN [2] RP 1-7601 RA Rott M.E., Belton M.; RT ; RL Submitted (21-AUG-2012) to the INSDC. RL Centre for Plant Health, Canadian Food Inspection Agency, Sidney RL Laboratory, 8801 East Saanich Rd, North Sanich, British Columbia V8L 1H3, RL Canada XX DR MD5; 74c36eeddde2ce0ea00e45f322337cce. XX CC ##Assembly-Data-START## CC Assembly Method :: CLCBio Genomic Workbench v. 4.5 CC Sequencing Technology :: Illumina CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7601 FT /organism="Grapevine virus B" FT /host="Vitis vinifera" FT /isolate="GVB 3138-01" FT /mol_type="genomic RNA" FT /country="USA" FT /collection_date="Sep-2010" FT /db_xref="taxon:35289" FT gene 49..5178 FT /gene="ORF1" FT CDS 49..5178 FT /codon_start=1 FT /gene="ORF1" FT /product="replicase" FT /note="contains methyltransferase (pfam01660), helicase FT (pfam01443) and RNA-dependent RNA polymerase (cl03049) FT domains" FT /db_xref="GOA:K4N9B4" FT /db_xref="InterPro:IPR001788" FT /db_xref="InterPro:IPR002588" FT /db_xref="InterPro:IPR005123" FT /db_xref="InterPro:IPR007094" FT /db_xref="InterPro:IPR027351" FT /db_xref="InterPro:IPR027417" FT /db_xref="InterPro:IPR027450" FT /db_xref="InterPro:IPR037151" FT /db_xref="UniProtKB/TrEMBL:K4N9B4" FT /protein_id="AFV34758.1" FT /translation="MSISVSSQRVASAALYQNGSTDQIEEIKKIKSSRLLSSEKHSDGL FT FDYYVPDFLRDYFARKGVHTSVHSFQAHPHPCSKMIENHILYNIVSQYVDDSTLFVSCK FT ESKLRTLFMKKTNKSLSSVTQYNRLVHAKDVLRYTDPIRELDMDHLTRLSELAKRAKTA FT FIHDEVHYWSLKDFQSFLGHLGGTQRLLYSIIYPAELHVGCEQSLFPEAYEFDLLGEYI FT TWYPDGKADGSYKQPVNPWLLTTSKTVDVHGQTWTLTKVLTMGAHHLFLATKGSSITEE FT EYHYDDYTVILPKKLLQGRRRNKMPYLRSRFITSVLLYLLALKKPDPSSAVAKLRQLTN FT GEESTAEALFVAQLSRQIQETKLYDKMGNFSLKECIWSAVSAAMGDSLVYFFDKARFYN FT ESLESFILNCGPAKIEVKRVFREAVYRNNGVCPHLASFDGNPDCIIDELYLQNIRDQDR FT MREPYSIPKGELVNGQTRCRISLIEMGRAVHRFHQERKVEINREVIVSPLDNFKALREP FT LTGRNGVIGMTCQLLTWEADPLKRYSMGLIDAKALNSALFGDRPPIPKERPAQEKEQSV FT FNCSCGVALPKMKLNGSVDVPIKEFTDALKGRKAAFYSRHSLGYNYNGGSHSSKGWPEW FT IDGIAKELGLGEEYDHCLAQVYEENKGINYHADDEPCYTDPEVVTVNLKGAAKFYLKCS FT TECSLSLEDGDVLVMPKGFQATHKHAVTSLTEGRVSLTFRNGINAPDEGSDKMSEYEEN FT SPELEESLDALEKNRKSLCALQILADHMRVDLAICTSMVFAKDPRAIEEVKRGGMTLGT FT FICVLKSLNLAAYIESERGCLVVNGTYKELSCYAEDDHIAAWTGPRKDTSFSNALSMNP FT DIRIVKYEVCEQRAKRLASSFQEGFTGVCLNKFQKQKSSFNLVNGKELIDVHLTLGFAG FT SGKSFYPQCVLKNSSYANALVIVPRKALCSDWTNKVHPDVKVVTFESAFRQQKKGYGLI FT VIDEIGLLPPGYIDMVHGYFCYDSLLVLGDPLQCEYHSKSDHFFLGQEESIFKSFKGHC FT NYLYKSHRLPKNQRLFEIECDGAEGEGLTFNKPRGKDLTLCASQRRKESEKNVSTVGES FT QGLSANRVNILLDRDWSLVNEETVVVAFTRARKEINVIGDTTLISNLKRSAKSTILRKI FT LSGERVTESLILSLIRRKLPECDLVKKEMLLGASDEMEEKLSGDPYLKGLLCLIDEIEE FT EEVEIPEPELLEPQRTHLPLSVGENELCISDLRAKEYREASTEAGRTDQIDDLGYKGEP FT ENPMNHKALYLYHQNSDVATFFLSVRKRLRFRDAEKNRRKFNKCRGFGAQMFGVMKQIY FT NLKQPDHLPLLEKAEQDFMKKRIAKSSKLIEKHSYRSEPDWPSNYLKVFLKQQRCTKME FT KRKVDAKAGQTIACFCHAVLCRFGPLLRQTERALRDQLGPNVMIYSQKNYTDLDRWCKN FT FVRTLDGTDSDYEAFDRSQDEKILDFEVNVLRFFLWPEEMIEEYVTLKLMMGCSMGGLA FT VMRFSGEFGTFFFNTVCNMGFTCLKYSIRADTPICYAGDDMYAPGVLVTKSEFKHILAE FT LQLKAKVNYTRSPLFCGWRMSPYGIVKDPNLLLDRWKIAERDGSLKNCMVNYALEAIYG FT YRLGEHLFDLNIDIDAQQDLVRRIVLIKHLLPQSLQRFYSDEERECYSDGEEFNLKVKN FT EGGLDESEFADD" FT gene 5153..5692 FT /gene="ORF2" FT CDS 5153..5692 FT /codon_start=1 FT /gene="ORF2" FT /product="20 kDa protein" FT /db_xref="UniProtKB/TrEMBL:K4MY99" FT /protein_id="AFV34759.1" FT /translation="MNQNLRMIEHDGLRRFLRQVEGFSLDINVLCRDYNKLGPLEAQQY FT RILSLLCRINKVSVPFVVSCLIKKECVVGDYQEGLVCLRLEEVIELLVSGLLPSVFTIR FT KDFERGSVAFNYPWYYSVTREPGSRLQYSFRNYVPGLKERIAALPWVALGFKASAANNP FT GNPGSHSYTIECVRQA" FT gene 5562..6527 FT /gene="ORF3" FT CDS 5562..6527 FT /codon_start=1 FT /gene="ORF3" FT /product="movement protein" FT /note="contains viral movement protein domain pfam01107" FT /db_xref="InterPro:IPR028919" FT /db_xref="UniProtKB/TrEMBL:K4MVL2" FT /protein_id="AFV34760.1" FT /translation="MSQVSRSESQRYPGWLSASKQAQRTTQGTPDPTHTQLSVFDKPKE FT ESGVKGVKSAITKAKVYDLSYFERLFPTRVFKSVVHEEIRIRDGEVKQVINLMDENFVK FT KIETTSYPYLHLGCLAICVIPHGRDLKGDVEFILRDTRFLPNSEPLMSFGCKAEKMLSA FT FSAFPGYFVSTFDLIDGFTMELEVKARGMEFEEGVRPMSIQIICVAKLCTQDFHHQYAL FT QKVKKGAYQALLNSYLVQVEDQTEYLEIQNPGKSLDRIGYTGIQVKSGESESIDKLLVP FT PEVYETISKQFPESNGKYIPDGKNKGEYLRTPVRGSHICN" FT gene 6448..7041 FT /gene="CP" FT CDS 6448..7041 FT /codon_start=1 FT /gene="CP" FT /product="coat protein" FT /note="contains pfam05892 trichovirus coat protein domain" FT /db_xref="GOA:K4MYM7" FT /db_xref="InterPro:IPR008879" FT /db_xref="UniProtKB/TrEMBL:K4MYM7" FT /protein_id="AFV34761.1" FT /translation="MENISRMARIRANISELLCAGVTFVTDARETGFDRPMYFRTLFGY FT IALTGTSAKAQHYENVDIIGDKVGAEGLDSRGTVNISEQVKKMMGYSRAVPSGVCKGLT FT LRQMCEPFAEEARDCLTILATLRVYSRLALKMAKLGQKEPQVMFDFNSGLNLLALSATE FT ASAIQSLNSRLFRTEGAKNVFTAQASVGEQSVEI" FT gene 7083..7457 FT /gene="ORF5" FT CDS 7083..7457 FT /codon_start=1 FT /gene="ORF5" FT /product="RNA-binding protein" FT /note="contains viral NABP super family cl05203, viral FT nucleic acid binding domain" FT /db_xref="InterPro:IPR008891" FT /db_xref="UniProtKB/TrEMBL:K4MWC3" FT /protein_id="AFV34762.1" FT /translation="MSKYMGESRSAAKRRATRYGRCYCCGRLECNGGRRTTISQDEVKA FT CIRTPAIRYLTENGGRYVTNAISLALSDVERVGKSEYQSLFKHNSAPIGMRSPEETPEF FT YNWGADFNAGSSVFADRPYK" XX SQ Sequence 7601 BP; 2250 A; 1472 C; 2011 G; 1868 T; 0 other; gaaaacaaaa cgctttttct ttaatcttcc gtaacgttac caaaagtaat gtcaatatcc 60 gtctcgtcac agcgtgttgc tagtgcagcc ctgtaccaga atggatctac ggaccagatt 120 gaagaaatta aaaaaattaa aagctccagg ctacttagct cggagaagca tagtgacgga 180 ttgtttgatt actatgtgcc tgatttcctg cgcgattatt tcgcaaggaa aggggtacat 240 acgagtgtac actcttttca agcacacccg cacccgtgct caaagatgat agaaaatcat 300 attctttata atatagttag tcagtatgta gatgattcaa ctctttttgt tagttgtaaa 360 gagtccaaac ttaggacgtt attcatgaaa aagaccaaca aatcattgag ttcagtcact 420 caatataaca ggttggttca tgctaaagac gttctgagat atacggatcc tataagggag 480 ttagacatgg accatttgac caggctatct gaactagcca aaagagcaaa aacggccttt 540 atccatgatg aggtccacta ctggagtctg aaggatttcc agagtttctt aggtcactta 600 gggggaactc aacggttgct gtacagcatc atttaccctg ctgagctcca cgtaggatgc 660 gagcaaagct tattcccgga ggcttacgaa tttgacctac taggagagta cataacatgg 720 taccctgacg gtaaagctga tggttcgtac aagcagccgg tcaacccgtg gttactcacg 780 acctctaaga cggtggatgt gcacggccag acatggacgt taaccaaggt actcaccatg 840 ggtgcacatc atctgttcct ggccacgaaa ggcagttcaa tcacagagga ggagtaccat 900 tacgatgatt acactgtgat cctaccaaag aaattgctac aaggtaggcg gaggaacaag 960 atgccctact tgagaagtag attcataacg agcgtgctac tctacttgct agccctaaag 1020 aaacctgacc caagctccgc agtggcaaag ttgcgtcagt taactaatgg ggaggaaagc 1080 acggccgaag cattgtttgt cgctcaactt tctaggcaaa ttcaggagac caaactctac 1140 gacaaaatgg gtaatttctc tctgaaagaa tgcatctgga gcgcggtatc tgcagccatg 1200 ggtgactcct tggtctattt ctttgacaag gcaaggtttt acaatgaatc gttggagagc 1260 tttatcctga actgcggccc agcgaagata gaggtgaaaa gagtattcag agaggctgta 1320 tacaggaaca atggggtttg tccacacttg gccagctttg atgggaaccc ggactgcatc 1380 attgatgaac tttatctcca aaatatcagg gatcaagacc ggatgagaga gccctactcc 1440 atccccaagg gtgagttggt gaatggtcaa acccgctgca ggatctcgct catagagatg 1500 gggcgagcgg ttcatagatt ccatcaagag aggaaggtgg agataaacag agaggtgata 1560 gtgagcccct tggacaattt caaggccctt cgagaacctc taactggtcg taacggtgtg 1620 attggcatga cttgtcaatt acttacttgg gaagcagatc cacttaaaag gtactcaatg 1680 ggccttattg acgccaaagc actcaattcc gctctgtttg gtgacagacc accaattcct 1740 aaagagaggc cagctcaaga gaaagagcaa agtgttttta attgcagctg cggggtggca 1800 ttaccaaaga tgaaattaaa tggatcagtc gatgttccca tcaaagagtt cactgatgcc 1860 ctgaaaggaa ggaaggcggc tttctatagc cgacactctc tgggctacaa ctacaatggg 1920 ggcagtcaca gcagcaaagg atggcctgag tggatagacg ggattgcgaa agaattgggc 1980 cttggagagg agtacgacca ctgcttggct caggtatacg aggagaacaa gggaatcaat 2040 tatcatgcag atgatgaacc ttgttacact gatccagaag tggtcacagt gaatctcaaa 2100 ggggctgcaa agttttattt aaaatgtagc actgaatgtt cattgtccct tgaagatggg 2160 gatgtgctcg tgatgccaaa agggttccaa gctacccaca agcatgctgt gacaagtttg 2220 acagaaggaa gggtgagttt gacattcagg aacgggataa atgcacccga tgagggtagt 2280 gacaagatga gtgagtatga ggaaaactca cccgaattgg aggagagcct agatgccctg 2340 gaaaagaatc ggaagagtct gtgcgctcta caaatattgg ctgatcatat gagggtcgac 2400 ttggcaatat gcacgagcat ggtgtttgcc aaagacccta gggctataga agaggtcaag 2460 agaggaggaa tgactctagg gacgtttatc tgtgtcctta agagcttaaa tctcgcagca 2520 tacatagagt ccgaaagagg ttgtttggtc gtgaatggga cgtataagga gcttagctgc 2580 tacgctgagg acgaccacat agctgcatgg actggcccca ggaaagatac ctcgttttca 2640 aatgctttat caatgaaccc ggatatcagg atcgtgaagt acgaagtgtg cgaacaaagg 2700 gctaagaggc tggcaagtag cttccaggaa ggctttacag gggtttgctt gaacaagttc 2760 cagaaacaga agagctcctt caatctggtg aatggtaaag agctaataga tgttcatcta 2820 actctaggat ttgctgggtc tggaaagagt ttctatcccc aatgcgtgct caagaacagc 2880 agttatgcaa atgcactcgt gattgtgcca aggaaggctc tctgtagcga ttggaccaat 2940 aaagttcacc cggatgtgaa agtggtgact ttcgaaagtg cattcaggca acaaaagaag 3000 ggttatggac tcattgtcat agatgaaata ggactactgc caccaggtta catagatatg 3060 gtgcacgggt atttctgtta tgatagccta ctggtgctag gtgatcccct tcaatgtgag 3120 taccacagca aatctgacca ctttttcctc ggtcaagaag agagcatctt taagagcttc 3180 aaaggccact gcaattactt gtacaaaagc cacaggctac caaagaatca gaggttgttc 3240 gaaatagagt gcgatggcgc agagggtgag gggttaacgt tcaataaacc cagaggaaag 3300 gatttgacgc tctgcgcatc acaaagaaga aaggagagtg agaagaatgt gagcactgtt 3360 ggagagtctc agggtttaag tgccaacagg gtaaacatcc tcctggatag agattggagc 3420 ttagtgaacg aggaaactgt ggtggttgcc ttcaccagag ccaggaaaga aataaatgtg 3480 atcggtgaca ccaccctgat atcgaacctc aagaggagtg caaaaagcac aatactcaga 3540 aaaatactga gtggtgagag agtaaccgag agtttgatac taagcttaat cagaagaaag 3600 ttacccgagt gtgacttggt gaagaaagaa atgctgcttg gtgcatcgga tgagatggaa 3660 gagaaactga gcggggatcc ctacctgaag ggattgttgt gcttgattga tgagattgag 3720 gaggaagagg ttgagatacc tgaacctgaa ttgcttgagc cacaaagaac tcatctacca 3780 cttagtgtgg gggaaaatga gttatgcata agcgatctca gggcgaaaga gtatagggag 3840 gccagtactg aggctggtag aactgatcaa attgatgatc ttggttacaa gggtgagcca 3900 gaaaacccta tgaatcataa agctctttat ttatatcatc agaactctga tgtggccaca 3960 ttctttctat ccgtgaggaa gagattgcgg ttcagggatg cagagaagaa caggcgtaaa 4020 tttaacaagt gccgtgggtt tggggcacaa atgtttgggg tgatgaagca gatttacaat 4080 ctgaaacagc ctgaccacct acctttgcta gagaaagcgg aacaggactt catgaagaaa 4140 aggatagcga agtcgtcaaa gctcattgaa aagcatagtt acagaagtga gccagattgg 4200 cctagtaact atctcaaagt cttcttgaag cagcaaaggt gcacaaaaat ggagaaaagg 4260 aaggtggacg ctaaagctgg gcaaaccata gcctgtttct gtcacgcggt tctgtgtcgc 4320 ttcggtccgt tgcttcgcca aacagaaaga gctctcagag atcaactcgg tccaaatgtg 4380 atgatttact ctcagaagaa ttacactgat ttggaccgat ggtgcaaaaa tttcgtccgg 4440 accttagacg gcacagattc tgactatgag gcgtttgaca ggagtcagga tgaaaagatt 4500 ctggattttg aagtaaatgt gttgaggttc ttcctatggc cagaagagat gatagaggag 4560 tatgtcaccc tcaagcttat gatgggttgt agcatgggtg gtctggcggt tatgcgtttc 4620 agcggtgaat ttggaacgtt cttttttaac accgtatgca acatgggatt tacgtgttta 4680 aaatacagca taagggcaga taccccaatt tgctacgctg gtgatgacat gtacgccccc 4740 ggggttctgg tgacaaagtc agagttcaag cacatcctag ctgagttgca attgaaagcg 4800 aaggtgaact acacgagaag cccactgttc tgtggttggc gcatgagtcc atacggaatt 4860 gtgaaggatc ctaatctgct cctggatcgg tggaagatag ctgaaaggga tgggagcctc 4920 aaaaactgca tggtaaatta tgctctagag gctatttacg ggtacaggtt aggtgagcac 4980 ctttttgatc tcaatataga tatagatgct caacaggatt tggttcgaag aatagtactc 5040 atcaagcatc tattgcccca gagccttcag aggttctact cggacgagga aagggagtgc 5100 tattctgatg gggaggaatt caatttaaag gtgaaaaacg aagggggcct ggatgaatca 5160 gaatttgcgg atgattgagc atgacgggct tcgtcgcttc ttgaggcagg tagaaggttt 5220 tagcttagat attaatgtat tgtgtagaga ttacaataag ttaggtccct tagaagctca 5280 gcagtatagg attttaagtc tcctttgtag gattaataaa gtgagtgtgc cattcgtagt 5340 gagctgtcta ataaagaaag agtgtgttgt tggtgattac caggaaggtt tggtttgtct 5400 acggttggaa gaagtgattg agctactcgt cagtggttta ttaccgtctg tcttcacaat 5460 tcgtaaggat tttgaaagag ggtctgtggc gttcaattac ccttggtact attctgtgac 5520 ccgcgaacca ggctcaaggt tgcaatattc cttccgcaat tatgtcccag gtctcaagga 5580 gcgaatcgca gcgttaccct gggtggctct cggcttcaaa gcaagcgcag cgaacaaccc 5640 agggaacccc ggatcccact catacacaat tgagtgtgtt cgacaagcct aaagaggagt 5700 caggggtgaa gggagtgaag agtgcaataa cgaaggcgaa agtgtacgac ttgagctatt 5760 ttgagaggct cttcccaacc agggtgttca aaagtgtggt ccacgaagag atcagaatca 5820 gggacgggga agtgaagcaa gtgatcaatc taatggatga gaattttgtg aaaaagattg 5880 aaactacttc ttacccttac ctgcacttgg gatgtcttgc gatatgtgtg ataccacacg 5940 gtagagatct gaaaggtgat gtagagttca tactccggga caccagattc ctaccgaaca 6000 gcgagccctt aatgtcgttt ggctgcaaag cggagaaaat gttgagcgcc ttcagtgcct 6060 tccctgggta tttcgtgagt acgttcgacc tgattgatgg tttcaccatg gaactggaag 6120 tgaaagccag gggaatggaa tttgaagagg gggtaaggcc aatgtccata cagattatct 6180 gtgtagcgaa gctgtgcaca caggattttc accatcagta tgctctgcaa aaggtgaaga 6240 aaggggccta tcaagcatta ctaaactcgt acttggtgca agtggaggat caaacggaat 6300 atctagagat tcagaatcca ggtaaatctc ttgacaggat cggttatacc ggtatccaag 6360 ttaagtctgg agaatctgag tcaatagata aactgttagt accaccggag gtgtacgaaa 6420 caataagcaa gcaattccct gagagcaatg gaaaatatat cccggatggc aagaataagg 6480 gcgaatatct ccgaactcct gtgcgcggga gtcacatttg taactgacgc ccgagaaact 6540 gggtttgatc gtcccatgta cttcaggacc ctattcgggt acattgcatt aactgggaca 6600 tcagcaaaag cgcagcacta cgagaatgtg gatattatag gtgataaagt aggagcagag 6660 ggtctagata gtaggggaac tgtcaatatc tcggaacagg tgaagaagat gatgggttat 6720 tctcgagcag taccatctgg ggtgtgtaag ggccttactc tgaggcaaat gtgtgaacct 6780 ttcgcagagg aagctaggga ctgcttgact atcctggcaa ccctgagggt gtacagcagg 6840 ctcgcactca agatggccaa actgggccaa aaagaacccc aagttatgtt cgactttaac 6900 tctggtctta acctgctcgc tctatccgct accgaggcat ctgcaataca atctctgaac 6960 tcaaggctct ttcgaactga aggtgcaaag aacgtattca cagcacaagc cagcgtgggt 7020 gagcagtctg tcgagatata ggtggttaac tcatagatat agtggtatag gagtccataa 7080 gtatgtctaa atatatgggt gagtctagaa gtgcggctaa acgaagggct actcgttatg 7140 gtcgctgtta ctgttgtggt agattagagt gtaatggagg gcgcagaact accatatcac 7200 aagatgaggt taaggcatgt ataaggacac cggcgattag gtatcttacc gaaaacggtg 7260 gtcggtacgt aactaatgcc attagcctcg ctcttagtga tgtggagagg gttgggaagt 7320 ctgagtacca atcattgttt aaacacaata gcgcgccaat aggtatgcgt agcccagaag 7380 agaccccaga gttctacaat tggggggccg atttcaacgc tggctcaagc gtgtttgctg 7440 atagaccata caaatagtct atccctgatt tcaatactag gtcaagtatg ttcgcttacg 7500 gaccgtacga atagtccgtc aataattcga gggtagaatt caaataatcc caccgctagc 7560 gacttaatgc taatggtgaa agaggcgtga ataaacacgg a 7601 //