Dbfetch
ID JX513891; SV 1; linear; genomic RNA; STD; VRL; 16489 BP. XX AC JX513891; XX DT 28-OCT-2012 (Rel. 114, Created) DT 28-OCT-2012 (Rel. 114, Last updated, Version 1) XX DE Grapevine leafroll-associated virus 2 isolate GLV2 1050-02 polyprotein DE (ORF1a), RNA-dependent RNA polymerase (ORF1b), 6.3 kDa small hydrophobic DE protein (ORF2), putative heat shock protein 70 (ORF3), 63 kDa putative DE cell-to-cell movemennt protein (ORF4), minor capsid protein (CPm), major DE capsid protein (CP), 19 kDa protein (ORF7), and 24 kDa protein (ORF8) DE genes, complete cds. XX KW . XX OS Grapevine leafroll-associated virus 2 OC Viruses; Riboviria; Closteroviridae; Closterovirus. XX RN [1] RP 1-16489 RA Rott M.E., Belton M., Saeed H.; RT "Detection of grapevine and tree fruit viruses by next generation RT sequencing"; RL Unpublished. XX RN [2] RP 1-16489 RA Rott M.E., Belton M.; RT ; RL Submitted (21-AUG-2012) to the INSDC. RL Centre for Plant Health, Canadian Food Inspection Agency, Sidney RL Laboratory, 8801 East Saanich Rd, North Sanich, British Columbia V8L 1H3, RL Canada XX DR MD5; 745496d79d2c67434503682479842992. XX CC ##Assembly-Data-START## CC Assembly Method :: CLCBio Genomic Workbench v. 4.5 CC Sequencing Technology :: Illumina CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..16489 FT /organism="Grapevine leafroll-associated virus 2" FT /host="Vitis vinifera" FT /isolate="GLV2 1050-02" FT /mol_type="genomic RNA" FT /country="Canada" FT /collection_date="Sep-2010" FT /db_xref="taxon:64003" FT gene 95..8890 FT /gene="ORF1a" FT CDS 95..8890 FT /codon_start=1 FT /gene="ORF1a" FT /product="polyprotein" FT /note="contains conserved domains for RNA methytransferase, FT two papain-like endopeptidases and a viral RNA helicase" FT /db_xref="GOA:K4N995" FT /db_xref="InterPro:IPR002588" FT /db_xref="InterPro:IPR008749" FT /db_xref="InterPro:IPR022054" FT /db_xref="InterPro:IPR027351" FT /db_xref="InterPro:IPR027417" FT /db_xref="UniProtKB/TrEMBL:K4N995" FT /protein_id="AFV34733.1" FT /translation="MSSLAISALPSAVAQLSFGQPAATVAKSFYMTSLPSLQTCFTQCE FT FSSLFICLASFQKIKTFLSFLSSVRELALFSELSTIGFCCEFIRLGVGEHPLFFVSFQC FT GPLSVCLGCVNGIFAVLNISFPFLDVASLLMDVGDVVAPTKVRISKREKKLNKRAFSIA FT RAAARIRRSKVGNKEVRISHVQPLPAGCLRFSQNKNRSELIIIGRDVGVVARVKTAAPV FT VRRRGSGGLVPLIPLVAVSPFGVRYRCYAPSCGAIACFKNKLASRVAANDSRAEKLLSA FT RRRKLQQQNFNSRVAKKARDVTASRVDIAEGEVTPPRHIFFGSFSEPFPIPSSSGMARS FT VLREEPAIVTCGASAPAIKETPRVSVKKADFSPAAPVPREALTPRVAKRGEAIVISGVV FT HEVLNKIREGVLRFRVGGDMRFSRFFSSTHGCKIVANVRTNTSVWLNCTKVNGEKFSVA FT IPCSVDYVAMLRYVSGGKFPLVLMSRECYPDGLCYMAHMRYLCAFFCQPFRESDYPLGS FT WPSVARLKALVLKRFGDEALNIGVRGYYVSRRAFHCDYDSKYARSLMRLVGHVGGDEVI FT HSTLASTVVLKPGERARDRPKSISFGSFCCELRYVESVNPDAPRLRSEKVLRKVEPRNG FT VRTESADVGSNVTRKRDARIDRKFSHLLAGSVNKVKKRIAAGVLRYRVGGDMDFHNSFL FT TQAGYHLLVRRKTSSSVCVELSTPDGRLLRRDVIPCSRDYAAMLSFAAGGRFPLVLMTR FT DKYKNGYCYLAHCRYASAFLLKGFHPAVFDIGANPTAAKLRSRMVSVLGDRSLSLNLYG FT SFTSRGIFHCDYDAAYVKDLRFMSAIVAGKEEMGEVVASDITPAMKQKTIEAVYDRLYG FT GNDSLLKLSIEKDLIDFKNDVQSLKKDRPVIRVPFYMSEVTQNSLTRFYPQFELKFSHS FT THSDHPAAAASRLLENETLVRLCGHSVSDIGGCPLFHLNSKTQRRVHVCRPVLDGKDAQ FT RRVVRELQYSSVRSGDDEKILEGPSNVDVCHYPLGVCEHESSTMMMVQVYDASLYEICS FT AMIKKKARVTYLTMVTPGEFLDGRECVYMESLDCDIEVDVHADVVMYKFGSSCYSHKLS FT IIKDIMTTPYLVLGGFLFSVEMYEIRMGVNYFKITKSEVCPNISCSKLLRYRRANSDVV FT KVKLPRFDKKRRMCLPGYDIIYLDSKFVSRVFDYVVCNCSAVNSKTFEWVWSFIKSSKS FT RVIISGKIIHKDVNLDLKYVESFAAVMLASGVRSRLASEYLAKNLSHFSGDCSFIEATS FT FVLREKIRNMTLNFNECLLQLVKRVAFATLDVSFLDLDATMESITDFAECKVAVELEEL FT GCLRANAENEKIRNLAGDSMAAKLASEIVVDIDAKPVAKHAEHPKSEVSEKKEHAKPGL FT RGGSKAGVLKEFLGFVLSSASRLFSCVADQQRIKSYVHYLDTAVSFLDQNYENLSFIMR FT VLSKGYSCMFSYLANRGSLANRVASAVQIVREIAVLCANVSVSKAKVVITFSAAVCAMM FT FNSCGFSGDGREYKSYMHRYTQVLFDTIFFEDSSYLPIELLSSAICGAIVTLFSSGTSV FT SLNAFLLQITKSFSLELIVRNVVRVTHSLSTTATDGVMRSVFSQIISHLLVGNTGNLAY FT QIAFLTGVVPLLVKKCVGLIFSMSEDTYSGFIKHGVSEFSFITNILKYLRGQLVDELKA FT VVQGVFDSNKHLFRDVTQEAIRSTVMQIPTAVVDGLKCAASKVYSGIVGKKTNVGEEKS FT RGGGDDYEEYFSCDESETPGLRGGSRSGFSILFFFSRLVRRARILIGRIKHRCFSKIFE FT SISLKIHELRTFLLGKKRSDVGVYEFLSEDVADALSSINECDEIENLIGDGFRDMADDS FT PTDTNYFDFSDEFLASVNGLPCAGLRGGGRRVGMLAILNYVYNSLSIVARAASKNSTFT FT SLIIFSRGLVDRIRNIFSNKESVVELVRKYVKNFLKSRMRGLGLRNEHVVNSFGELVPL FT LLRNKLSETFIYRAVPMSKWCWEVCLSAERREKFFALVCLVTLGFPYFARLLGGVVCDK FT LLSSLRRAYDRVIVFLKERCDAAMNGLRCLFHFDGNGDSTSAGLKGGGSQTFLWGLLLR FT LVRFALSLGYAGCKCVFSHHNLFFLCVSLVEEVNMIAKILGGLSFFSRPISSALAFVLL FT QPERFVEYSGKLSTTFLFFLKISPRVPLKIKGFFEWVAGNPLLRGVRRFLRLIAVKLNL FT RRKRGLRTRNGGYPSQRAVPVIPSNRVTNDGCERLSEKMQGVEALRSELRILEELDAAV FT VEKLNKRRNREADDSEFSRPMHESTHDVTSFCSKANSVGTALERAVLVEDAVKSEKISR FT MAKDLVRKGTVSDEEITNTLSDDEAIEEVPVSEERDDSPKTVRVSEYLNRLNSSFEFPK FT PIVVDDNKETGGLTNAVREFYYMQELALFEIHSKLCAYYDQLRIVNFERSMAPCNEDAQ FT LYVRKNGSTIVQGKDVRSNIKDFHDHDFLFDGKISVNRRRRSGNVLYHDNLSFLASNLF FT LAGYPFSRSFVFTNSSVDILLYEAPPGGGKTTTLIDSFLKVFKKGEVSTMILTANKSSQ FT VEILKKVEKDISNIECQKRKDKRTPKKSIYTIDAYLMHHRGCDADILFIDECFMVHAGS FT VLACIEFTRCHKVMIFGDSRQIHYIERNELDKCLYGDLDRFVDLQCRVYGNVSYRCPWD FT VCAWLSVVYDNMIATVKGESEGKSSMRINEINSVDDLVPDMGSTYLCMLQSEKLEISKH FT FIRKGLSKLNVLTVHEAQGETYARVNLVRLKFQEDEPFKSIRHITVALSRHTDSLTYNV FT LAARRGDATCDAIQKANELVNKFRVFPTSFGGSVINLDVKKDVEDNSRCKASSAPLSVI FT NDFLSDVNPGTTVIDFGDLSADFSTGPFECGASGIVVRDNISSSNITDHDKQRV" FT gene 8766..10268 FT /gene="ORF1b" FT CDS 8766..10268 FT /codon_start=1 FT /gene="ORF1b" FT /product="RNA-dependent RNA polymerase" FT /db_xref="GOA:K4MY79" FT /db_xref="InterPro:IPR001788" FT /db_xref="InterPro:IPR007094" FT /db_xref="UniProtKB/TrEMBL:K4MY79" FT /protein_id="AFV34734.1" FT /translation="MISEISLQILARDLSSVALAASSCETTSPPATSLITISSVFSAVR FT SQAIPRRKPSLQENLYSFEARNYNFSTCDRFTSASMFGEAMAMNCIRRCFDLETFSSLR FT DDVISITRSGIEQWLEKRTPSQIKALMKDIESPLEIDDEICRFKLMVKRDAKVKLDSSC FT LTKHSAAQNIMFHRKSINAIFSPIFNEVKNRIMSCLKPNIKFFTEMTNRDFASVVSNML FT GDDDVYHIGEVDFSKYDKSQDAFVKSFEEVMYKELGVDEELLAIWMCGERLSIANTLDG FT QLSFTIENQRKSGASNTWIGNSLVTLGILSLYYDVRHFEALYVSGDDSLIFSRNKISNY FT ADDICADMGFETKFMSPSVPYFCSKFVVMCGHKTFFVPDPYKLFVKLGAVKEDVSMDFL FT FETFTSFKDLTSDFNDERVIQKLAELVALKYDVQSGNTTLALSVIHCLRSNFLSFSKLY FT PRVKGWQVFYTSVKKALFKSGCSLYESYVTPFGHAVMVWDDE" FT gene 10361..10531 FT /gene="ORF2" FT CDS 10361..10531 FT /codon_start=1 FT /gene="ORF2" FT /product="6.3 kDa small hydrophobic protein" FT /db_xref="GOA:K4MVJ0" FT /db_xref="UniProtKB/TrEMBL:K4MVJ0" FT /protein_id="AFV34735.1" FT /translation="MDQVLQFECLYLLNQAVFAITFVFILLIIRVIKSFRRNVHETPIS FT AVRGQGFSTVV" FT gene 10540..12339 FT /gene="ORF3" FT CDS 10540..12339 FT /codon_start=1 FT /gene="ORF3" FT /product="putative heat shock protein 70" FT /note="HSP70h" FT /db_xref="GOA:K4MYL0" FT /db_xref="InterPro:IPR013126" FT /db_xref="InterPro:IPR018181" FT /db_xref="InterPro:IPR029047" FT /db_xref="UniProtKB/TrEMBL:K4MYL0" FT /protein_id="AFV34736.1" FT /translation="MVVFGLDFGTTFSTVCVYKDGKVYSFKQNNSAYIPTYLYLFSETN FT HMVFGYEAESLMHNAKVRGSFFRDLKRWVGCDSSNFSDYFDRLKPHYAVRPYKIGTGLN FT DTVIIGNYGGTVRSEVHLPGLIASFIKAMVSCAENAFSCTCTGVICSVPANYDSVQRNF FT TDQCVSLSGYQCVYMVNEPSAAALSACNLINKKSANLAVYDFGGGTFDVSIISYRNNTF FT VVRASGGDLNLGGRDVDRAFLTHLFSLTSLEPDLSLDISNLKESLSKTDAEIVYTLKGS FT DDKREDVRVNKSILTTVMLPYVNRTLNILEATLKSYAKNMGGLRSVKCDLVLIGGSSYL FT PGLEDILSKHRRIDRILKVADPRAAVAVGCALYSSCLSGSQGLLLVDCAAHTIAIAGMS FT CDQIICAPAGAPIPFSGTTPLYLPKANRNSQRQIAVFEGEYVKCPRNRKICGSNIKFLD FT IGVTSVSHSPITFYLDVAISSVGAISFVVRGPEGKEVSLTGTPAYNFSSVVLGTRSVRE FT LHISLSNKVHLGLLLHRKADRRLLFSEGEAVRYVETVEVADVLKEFKSYNASSLPPDED FT VEPLMGKSVQKVLRGGRLEEIPL" FT gene 12266..13870 FT /gene="ORF4" FT CDS 12266..13870 FT /codon_start=1 FT /gene="ORF4" FT /product="63 kDa putative cell-to-cell movemennt protein" FT /note="similar to viral heat shock protein Hsp90" FT /db_xref="InterPro:IPR004909" FT /db_xref="UniProtKB/TrEMBL:K4MW99" FT /protein_id="AFV34737.1" FT /translation="MSNHSWGSLFKKYYGEADWRKYLSKSIVAHTANIKTLPDVQLYGG FT RTVKKSEFESVTPRSFEQELGLLMLSERDVGWCKMCGITVEEAMYQITNPEAYPITAEK FT CDPDVKTEGQKYTMEDVMNFMRRSNLDINDKILAEQCWSLSNSCGVLINPDDKERFVAL FT TFKNNELVNDSSNANIDCRVGDYLVYAMSLFNCRSQKSQAGNISLYEKYCAYIRTYLEN FT TDLYFTSPDRIPLLTGILYDFCKEYNIFYSTYKRNVDNFRFFLTNYMPLISDIFVFQWV FT KPAPDVRLLFDISAAELTLEVPTLSLVDSQVVIGHVLRFVESYTADPAIDALEEKLDAI FT MKKSNPHLSTAQLWVGFFCYYGEFRTAQRRVVQRPGVYKTPNSVGGFEINMKNVEEFFD FT KIQREVPNVSLRRQFNGARAHEAFKVFKKGNISFKPISRLNIPREFWYLNVDYFRHANR FT SGLSEEEVLILNNISVDVRKLCAERACSTLPSAKRFSKGHKSSVPSLRQEKNYRDPLIT FT LRNSLYEFRHGNRDRRV" FT gene 13833..14504 FT /gene="CPm" FT CDS 13833..14504 FT /codon_start=1 FT /gene="CPm" FT /product="minor capsid protein" FT /db_xref="GOA:K4N999" FT /db_xref="InterPro:IPR002679" FT /db_xref="UniProtKB/TrEMBL:K4N999" FT /protein_id="AFV34738.1" FT /translation="MSSGTGIAIGGSEAIETSGFVLSTRKEAVDKFFNELRNENYSSVD FT SSRLSDQEVKEVLEKSKESFKSELASTDEHFVYHIIFFLIRCAIISTSDKVKYVGSHKY FT VVEGKTYTVQDVWVFNMIKGLTKQYKRVNGLRAFCCACEDLYLTVAPAMSERFKTKAIG FT MKGLPVGKEYLGADFLSGTSRLMSDHDRAVSIVAAKNAVDRSAFTGGERKIVSLYDLGR FT N" FT gene 14592..15188 FT /gene="CP" FT CDS 14592..15188 FT /codon_start=1 FT /gene="CP" FT /product="major capsid protein" FT /db_xref="GOA:K4MY83" FT /db_xref="InterPro:IPR002679" FT /db_xref="UniProtKB/TrEMBL:K4MY83" FT /protein_id="AFV34739.1" FT /translation="MELMSDDNLSGLVITDASSLNGADKKLLSAEVIKMLVQKGAPSEG FT IEIVFGLLLYALAARTTSPKVQRADSDIIFQNTYGDKTVVVTEGDLKKVLEGCAPLNRF FT TNKPRTFGRTFTEAYIDFCVAYKHKMPQLNAAANLGIPAEDSYLAADFLGACPKLSELQ FT QSRKMFASMYALKTEDGVVNTPVSNLRQLGRREVI" FT gene 15185..15673 FT /gene="ORF7" FT CDS 15185..15673 FT /codon_start=1 FT /gene="ORF7" FT /product="19 kDa protein" FT /db_xref="UniProtKB/TrEMBL:K4MVJ5" FT /protein_id="AFV34740.1" FT /translation="MMEICAQNPEALVLLRTNQNTTLLVVKSDDEVNLPKLLICGYLRV FT SHRGDVTGCNREDIIKDFEGAHHTVIRSRTVQYDYESAVKEYNNADCVVKFFLETGDVF FT WFFLQSDIKGRAARHLRTFFEANNFFFGSHCGTMEYCLKQVLIETESVIESFCEERNR" FT gene 15675..16292 FT /gene="ORF8" FT CDS 15675..16292 FT /codon_start=1 FT /gene="ORF8" FT /product="24 kDa protein" FT /db_xref="InterPro:IPR021575" FT /db_xref="InterPro:IPR021742" FT /db_xref="UniProtKB/TrEMBL:K4MYL3" FT /protein_id="AFV34741.1" FT /translation="MRVIVSPYEAEDILTRSTEMLRNIDSGALDTKECIEAFSTLTRDL FT HCAKASYQWCVDTGLCRRDCAEKRLIDTVESNLRLAQPLIREKVATHFCKDEPKELVAF FT ITRKYVKLTGVAAREAVKIEMRTLSKTVLNKLSLEMAFYISPRAWKNAEWLDLRYTPLK FT IYRDLLLDVDTLNALCEEDEVHVVVTNKDEDENHDLELQEDC" XX SQ Sequence 16489 BP; 4236 A; 3422 C; 4213 G; 4618 T; 0 other; ctttcacctg tgtttgtgcc tcggttcaac cacaacctga aagtttcagt tcccaggagt 60 atcggttttt cctcagaaac cttacttctt cagaatgtct agccttgcta tttctgctct 120 tccctccgcg gttgctcaat tgagttttgg tcagcctgct gccacggttg ccaagtcatt 180 ttacatgact tcccttccgt cccttcagac ttgcttcact cagtgcgagt tttcctctct 240 ttttatttgt ttggcttctt tccaaaaaat aaaaacattt ctttctttcc taagttcagt 300 gcgtgaactc gctctttttt ctgaactgtc cacgattgga ttctgctgtg aattcattcg 360 cttaggtgtt ggagaacatc ctttgttttt tgtttccttc cagtgcggac ctttgtccgt 420 gtgtttgggg tgtgtgaatg gtatttttgc cgttctcaac atttcttttc cctttctcga 480 cgttgcttcg ttgttgatgg acgttggtga tgtggtcgct ccgaccaagg taagaatttc 540 caaacgagag aagaagttga ataagcgcgc tttttcaata gctcgtgctg ctgcgcgtat 600 taggcgttcc aaggttggaa ataaggaggt tcgcatctct catgtgcagc ccctccctgc 660 tggttgctta cggttttccc aaaacaaaaa cagatcagaa ctcatcataa taggtcggga 720 cgtaggtgtt gtggcgcgcg taaaaaccgc agcacctgtt gtgcggcgtc gcggtagcgg 780 cggtcttgtt cccctcatac ccctagtagc cgtttcgcct tttggggtga ggtaccgttg 840 ttacgcgcct tcatgtggcg cgatcgcgtg tttcaagaat aagctcgcta gccgtgttgc 900 cgctaacgat agtcgcgccg agaagcttct ttcagctcgg cgccgtaaat tgcagcaaca 960 aaatttcaac agtcgtgtgg cgaagaaagc gcgtgacgta actgcgtcgc gcgtcgatat 1020 agcagaaggt gaagtcacac caccgcgcca catctttttt ggtagttttt cggaaccctt 1080 tccaatacct tcttcttccg gaatggcgag atcggtgctt cgtgaagaac ccgctatcgt 1140 tacgtgtggc gctagcgcgc ccgcgataaa agaaactccg cgggtgagtg tgaaaaaggc 1200 ggatttttca ccggcggcgc ctgttccgcg ggaggctctt accccaagag tcgctaaacg 1260 cggtgaagcg atcgtcatat cgggcgtggt gcacgaagtt ttgaataaga ttagagaggg 1320 cgttttgcgc tttcgcgtgg gcggtgatat gcgtttctct aggttcttct catcaaccca 1380 cggttgcaaa attgttgcca acgtgcgcac caacacttcc gtgtggttga actgcactaa 1440 ggtcaatggt gaaaaatttt cggtggcgat cccttgttcg gtggattatg tagcgatgct 1500 gcgttacgtg agtggcggga agttcccgct tgtcctgatg agcagggaat gttaccctga 1560 tgggctatgt tacatggctc acatgaggta tctgtgtgct ttcttttgcc aacctttcag 1620 agaatcagac tatcctctgg gttcgtggcc atcggtggcg cgattgaagg cgttggtttt 1680 gaagcgcttt ggtgacgagg cgctgaatat aggcgttcgc ggctactatg tctctcgtag 1740 ggcttttcat tgcgactacg attctaaata cgcgagaagc ttgatgaggc ttgtggggca 1800 tgtcggggga gacgaggtta tacattcaac gttggcgtcc acggtcgtgt taaaaccggg 1860 tgaacgcgca cgcgatcgtc cgaaaagcat aagttttgga tcgttttgct gcgagttgcg 1920 ttacgtcgag tccgtcaatc ctgacgcccc tcgcttgcga agtgagaagg ttctgaggaa 1980 agtggagccg cgcaatgggg ttcgcacgga aagcgctgac gttggttcga acgtcacacg 2040 gaagcgcgac gcccgcatcg atagaaagtt ttcgcatctt ttagcgggaa gcgttaataa 2100 agtgaagaaa cgaatcgcgg ccggggtgtt gcgatatcga gtcggcggtg acatggactt 2160 tcataactcc tttcttaccc aagcgggcta ccacttgcta gtgcggcgta agacgagcag 2220 cagtgtttgc gtggaacttt cgacgccgga cggtaggctc ttgcgccgcg acgtcatacc 2280 ctgctcaaga gattacgctg cgatgttgtc gtttgccgca ggaggtcgtt tcccgttggt 2340 tttaatgacg agggataaat acaagaacgg ttattgttac ttggctcact gtaggtacgc 2400 gagcgctttt cttctgaagg gttttcatcc tgcggttttt gacattggcg ctaacccgac 2460 ggcggcgaag cttagatcgc gcatggtttc ggtcctaggg gacagaagtc tgagtttgaa 2520 tttatacggg tctttcacgt cccgcggtat ttttcactgt gattacgacg cggcttatgt 2580 gaaagattta agattcatgt cagcgatcgt ggctggtaag gaggaaatgg gagaggtggt 2640 tgcttctgat ataactccgg cgatgaaaca gaagactatc gaagctgttt acgataggtt 2700 gtatggtggt aacgattcgt tgttgaaatt gagcatagag aaagatctaa tagatttcaa 2760 gaatgacgtt cagagcctga agaaggatag acccgttatt agggtgcctt tctacatgtc 2820 tgaagtcact cagaactcgc tgacgcgttt ctatcctcaa ttcgagctta aattctcaca 2880 ctctacgcac tctgatcatc ccgcggcggc cgcttcgagg ctgctagaga acgaaacgtt 2940 agtgcgattg tgcggtcaca gtgtttcgga cattgggggt tgtcctctct tccatcttaa 3000 ctcaaaaacg caaaggcgcg ttcacgtgtg ccgacccgtg ttagacggta aagacgcgca 3060 gcgtcgagtg gtgcgcgaat tgcagtattc ttcagtacga tcgggagatg atgagaaaat 3120 tttggaaggg ccctccaatg tggacgtgtg tcactaccct ctcggcgttt gtgagcacga 3180 gagtagtacc atgatgatgg tgcaggttta cgatgcatcg ctctatgaga tatgcagtgc 3240 tatgattaag aagaaggcgc gcgtaactta cctcactatg gtcactcctg gcgagtttct 3300 cgacgggcgt gaatgtgttt acatggaatc gctagactgc gatattgagg ttgatgtgca 3360 tgcggatgtt gtgatgtata agttcggtag ttcgtgctat tcgcataagt tatcgatcat 3420 taaagatatt atgaccacgc cgtatcttgt gctcgggggc tttttattta gtgtggaaat 3480 gtacgaaatc cgaatgggcg tgaactactt caagatcacg aagtcggaag tgtgccccaa 3540 tatctcatgt tctaagctgc tgagataccg cagggctaac agcgacgtcg tgaaagtgaa 3600 actgccgcgt tttgataaga agcgtcgcat gtgtttaccc gggtacgata ttatatactt 3660 agattcaaaa tttgtgagtc gtgttttcga ctacgtagtg tgtaattgtt ccgcggtgaa 3720 ctcaaagact ttcgaatggg tctggagttt cattaagtcc agcaaatcga gagtgattat 3780 tagtgggaag atcattcata aagatgtcaa ccttgatctc aagtacgtcg agagtttcgc 3840 tgccgtgatg cttgcgtcgg gtgtgcgcag caggttggct tcggagtatc tcgcgaagaa 3900 tctcagtcat ttttcgggag attgctcctt tatcgaagcg acctcttttg ttctgcgtga 3960 gaagattaga aatatgaccc ttaacttcaa tgaatgtctt ctgcagttgg tgaaacgcgt 4020 ggcatttgcg actttagacg taagtttctt agatctggat gccactatgg aatcaatcac 4080 agacttcgcc gaatgcaaag tagctgtgga acttgaagag ttgggatgtt tgagagcgaa 4140 tgccgagaat gagaaaattc ggaacctcgc tggcgactca atggcagcga aattggctag 4200 cgagatagtg gtcgacatag acgcgaaacc tgtcgctaaa cacgcagaac acccgaagag 4260 tgaagtgagt gaaaagaagg agcacgcgaa acccggcttg cgtggaggct cgaaagccgg 4320 agttttgaaa gagttcctcg gtttcgttct ttcgtccgcg tcgcgcttgt tcagttgcgt 4380 ggcggatcaa caacgaatta agtcgtacgt ccattacctt gacaccgctg tatcgttctt 4440 agatcagaat tacgaaaacc tctccttcat tatgcgcgtt ctatcgaaag gttactcttg 4500 catgttttca tatctcgcga atcgtggtag tcttgccaat cgcgtggcga gcgcggtgca 4560 aatcgttaga gaaattgctg tgttatgcgc taacgtaagc gtttccaagg ccaaagtagt 4620 gatcactttt tccgcagcgg tgtgcgcgat gatgttcaac agttgcggct tttcaggcga 4680 cggtcgagaa tataaatcat acatgcaccg ttacacgcaa gtgttgttcg acaccatttt 4740 ctttgaagat agtagctatc ttcccataga gttactcagt tccgcaatat gcggcgctat 4800 cgtcactctt ttctcgtcag gaacttctgt aagtctgaat gctttcctgc tccaaattac 4860 taagagcttc tctttggagc ttatcgtccg gaacgtggtg cgcgtgacgc attctttgag 4920 caccacggcg actgatggag tgatgcgcag cgtcttctct caaataatca gtcacctgct 4980 tgtgggaaac actggtaact tagcttatca aatagctttt ttaaccgggg tggtgccctt 5040 gttagtgaag aaatgcgtgg gtttgatttt ttcgatgagc gaggacacat actctggctt 5100 tatcaagcat ggggttagtg aattttcgtt catcactaat atcttaaaat acctgagagg 5160 acagctggta gacgaattga aagcggtagt tcaaggagtt tttgactcga ataaacatct 5220 ctttcgtgat gttactcagg aggcgatccg gtcgaccgtt atgcagatac caaccgccgt 5280 ggtggatggt ttgaaatgtg cggctagtaa agtgtatagc ggaatagtcg gtaaaaagac 5340 gaatgttggg gaagaaaaat cccgtggcgg tggagacgac tacgaagagt atttttcctg 5400 cgacgagagc gaaactccgg gtttaagggg cggatccaga agcggtttct ccatcctatt 5460 tttcttttca aggttagtgc ggcgagcgcg cattttgatt ggcagaataa agcataggtg 5520 cttctcgaaa atttttgaga gtatctcgtt gaagattcat gagcttagaa cctttctcct 5580 tggaaagaag cgaagcgacg taggggtgta cgaattccta tccgaggacg tagctgatgc 5640 tctatcctcg ataaatgaat gtgacgaaat cgaaaatctg atcggagacg ggtttcgcga 5700 tatggcggat gactctccca cggacacgaa ctacttcgac ttctcagacg aatttttagc 5760 ctccgttaat ggcttacctt gcgctgggtt gcgcggaggc ggtaggaggg tgggaatgct 5820 cgccattctg aattacgttt ataactctct aagcatcgtg gctagagcag cttcgaaaaa 5880 ttcgactttc acatctctaa tcattttttc gcgcggattg gttgatagaa tccgcaacat 5940 tttttcaaat aaggaaagtg tcgttgagct tgtgcgcaag tacgttaaaa actttttaaa 6000 aagtcgcatg cgcggtttgg gtctaagaaa cgaacatgta gtgaattcgt tcggggagtt 6060 ggtgccactg ctcttacgaa acaagttgtc tgaaactttc atctatcgtg cggtccccat 6120 gtcgaagtgg tgctgggaag tgtgcttgtc agctgagcgg cgggaaaagt ttttcgcgct 6180 cgtgtgctta gtcactctgg gatttcccta cttcgctcgt cttttgggtg gggtagtgtg 6240 cgataagctc ttgtcttcat tacgacgtgc gtacgaccgt gtgatagttt ttctaaagga 6300 gcgatgtgac gcagccatga atgggttgcg ttgtttattt cattttgacg ggaatggtga 6360 ttccacatct gccggtttaa agggcggtgg ctcgcaaacg tttctatggg gcttgttatt 6420 acgtttagtg cgttttgcgc tatctttggg ctacgcgggt tgtaagtgcg ttttctcgca 6480 ccacaatcta tttttcttat gcgtctcctt agttgaagag gtgaacatga tcgcgaaaat 6540 tctagggggt ttgtcgttct tctcacgacc aatatcatca gcgctggcat tcgttctttt 6600 acagcccgag aggttcgttg agtactctgg aaagttgagc acgacttttt tgttttttct 6660 caaaatttct cctcgcgtgc ctctcaagat caaagggttt ttcgagtggg tagccggtaa 6720 tcctctattg aggggcgttc ggagattcct tcgcttgatc gcggtgaagc tgaaccttcg 6780 aaggaagaga ggactgagaa cgagaaatgg tggttacccc agccaaaggg ccgtgccggt 6840 tataccatct aatcgcgtga caaatgacgg atgtgagaga ctgtctgaga agatgcaagg 6900 agtcgaagca ctacgatccg aattaaggat tcttgaagaa cttgatgccg cagtggtgga 6960 gaagctcaac aaacgcagga accgtgaagc ggatgatagc gagttctcgc gccctatgca 7020 cgagagcacg catgatgtga cttccttttg ttcaaaagcg aattcggtag gcacggccct 7080 cgaaagggcg gtgctcgtgg aagatgcggt gaaatctgaa aagatctcga ggatggctaa 7140 agatctggtt cgtaagggga ctgtttccga tgaggaaatc accaacactt tatctgatga 7200 tgaagcgatt gaagaagttc cagtctccga agagcgcgac gactcaccca aaacggtaag 7260 ggtgagtgaa tatttgaata gattgaactc aagcttcgaa ttcccgaagc cgatcgtggt 7320 ggacgacaat aaggagactg gagggttaac caacgcggta cgcgaattct actatatgca 7380 ggagctcgct ctatttgaaa tccacagcaa actgtgcgct tactacgatc agttgcggat 7440 cgtgaatttc gaacgctcta tggccccatg taatgaggat gctcagttgt acgttcgtaa 7500 aaacggttcc acaatagtgc agggcaaaga tgtgcgttcg aacatcaagg attttcacga 7560 tcacgatttt ctcttcgatg gcaagatctc cgtaaataga cgacggcgat ctggaaacgt 7620 tctgtaccat gataaccttt cttttctggc gagcaacctg tttctggcgg gatacccttt 7680 ttcacggagt tttgttttca ctaactcttc ggtggatatt cttctttatg aagctcctcc 7740 cggtggtggc aagaccacga ccctaataga ttccttcctg aaagttttca aaaaggggga 7800 ggtatccacc atgatactaa ccgcgaacaa aagctcgcag gttgagattt taaagaaggt 7860 tgagaaagat atttccaata tcgaatgcca gaaacgcaaa gataagagaa ctccaaagaa 7920 gagcatatac accatcgacg cgtatttgat gcaccacaga ggttgtgatg ctgacatact 7980 ttttatcgat gagtgcttta tggttcacgc cggtagtgtg ttggcgtgta ttgaattcac 8040 taggtgccac aaagttatga tcttcggaga cagtcgacag attcattata ttgaaagaaa 8100 tgaactcgac aagtgccttt acggagacct tgacaggttc gtagacctgc aatgtcgtgt 8160 ctacggtaac gtgtcgtacc gctgtccatg ggacgtctgc gcctggttaa gcgttgttta 8220 tgacaacatg atcgccaccg ttaagggtga aagcgaagga aaaagtagca tgcgcattaa 8280 cgaaatcaac tcggtggacg acttagtccc cgacatgggt tctacttact tgtgcatgct 8340 tcagtctgag aagttagaaa tcagtaagca tttcatccgc aaagggctct ctaaacttaa 8400 tgtcttgacc gtgcacgagg ctcaaggtga gacatacgcg cgcgtgaatc tggtgagatt 8460 gaaatttcag gaggacgagc cttttaaatc catcaggcat ataaccgtcg cattatctag 8520 acacaccgac agcttgactt acaacgtcct agccgctcgg agaggcgacg ccacgtgcga 8580 cgctatacaa aaagctaacg agttggtgaa caagtttcgt gtcttcccga cttctttcgg 8640 tggtagcgtt atcaatctcg acgttaagaa agatgtggag gacaacagta ggtgcaaagc 8700 ttcttcggcg cctttaagtg tgatcaacga cttcttgagc gacgttaatc ccggcaccac 8760 cgtaattgat ttcggagatc tctctgcaga ttttagcacg ggacctttcg agtgtggcgc 8820 tagcggcatc gtcgtgcgag acaacatctc ctccagcaac atcactgatc acgataagca 8880 gcgtgtttag cgcagtgcgg tcgcaggcga ttccgcgcag aaagccttcc ctgcaagaga 8940 atctttattc ttttgaagcg cggaattaca acttctcgac ttgcgaccgc tttacctcag 9000 cttctatgtt cggtgaagcg atggctatga actgcatacg ccgctgtttt gatttagaga 9060 ccttttcatc tttgcgcgat gatgtgattt ctatcacaag gtcgggtatt gaacagtggt 9120 tggagaagcg tactcccagt caaataaagg ctttgatgaa agatatcgaa tcaccgctgg 9180 aaattgatga tgagatctgc aggttcaaat tgatggtgaa gcgtgatgct aaggtgaagt 9240 tagattcttc ttgcctaacc aaacacagcg ctgctcagaa cattatgttt catcgtaaaa 9300 gtatcaacgc gattttttca cccattttta acgaagtgaa gaaccgtatc atgagctgtc 9360 ttaagcccaa cataaaattc ttcaccgaga tgactaatag ggattttgct tccgttgtga 9420 gcaacatgct cggtgatgac gatgtttacc atataggcga agtcgatttc tcgaagtatg 9480 acaagtctca ggatgctttc gtgaaatcct ttgaagaagt catgtacaaa gaactcgggg 9540 ttgacgagga actcttagcg atttggatgt gtggtgaaag gctttccatt gcgaatacgc 9600 ttgacggtca gctgtctttc acgattgaga accagagaaa gtcgggtgcc tcaaacacgt 9660 ggatcggtaa ttctcttgtt acgctgggca tactaagtct ctattatgac gtgaggcatt 9720 ttgaggctct ctacgtctct ggtgacgact ctctgatttt ttcacgcaac aaaatttcga 9780 attacgctga tgatatctgc gctgatatgg gtttcgaaac gaagtttatg tctccgagtg 9840 ttccgtattt ttgttccaaa ttcgtcgtta tgtgcggaca caagaccttt tttgtgcctg 9900 atccgtataa gttattcgtg aaactgggag ctgtaaagga ggatgtctct atggattttc 9960 tctttgaaac tttcacctcc tttaaggatt tgacttcgga ttttaacgat gagcgcgtga 10020 ttcagaaact cgctgagctt gttgctctca aatacgacgt tcagagtggt aataccactc 10080 tagcgttgag cgtgatacat tgtttgcgtt ctaactttct atcgttcagc aaactgtacc 10140 ctcgtgtgaa aggatggcag gttttttaca cctccgttaa gaaagcactt tttaagagcg 10200 ggtgctcact ctacgaaagc tacgtcactc cattcggtca tgcagtgatg gtgtgggatg 10260 atgagtagca cgctactctt gcgtagtttt gaacggtact gtttccgcga ctgtgggatg 10320 atcacgtgac atacacgatg tgtgtcactg tatagatatt atggaccagg ttctgcagtt 10380 cgagtgtctg tatctgctca accaggcggt ttttgcaata acattcgtct tcattctgct 10440 catcattcgc gtgattaaat ctttccgcag aaacgtacac gaaactccaa tatcggccgt 10500 ccgtggtcag ggtttctcga ctgttgtgtg atttgcgaaa tggttgtttt tggtttagat 10560 ttcggtacga ctttttcgac ggtctgcgtg tataaagatg gtaaggttta ctcattcaaa 10620 caaaacaatt ctgcgtacat tccgacatac ctctaccttt tctccgaaac taatcacatg 10680 gtttttggtt atgaagccga atcactgatg cataatgcga aggtgagagg ctctttcttt 10740 agggacttaa aacgctgggt gggttgcgac tccagcaact ttagtgacta ctttgatcgc 10800 ctgaagccac actatgcggt tcgcccgtat aaaataggta ctggtctgaa tgatacggtg 10860 attataggaa actacggggg taccgttcgt tccgaagtgc atttgccagg actaatagct 10920 tcctttatca aggcgatggt tagttgtgcc gagaatgcct tttcttgcac ttgcacggga 10980 gtaatatgtt cagtgccagc gaattatgac agtgttcaga ggaatttcac tgatcagtgc 11040 gtttccttga gtggttatca atgtgtgtac atggtcaacg aaccttccgc ggctgcttta 11100 tcagcgtgca atctgataaa caagaagtcg gcgaacttgg ctgtatacga ttttggaggt 11160 gggactttcg acgtgtctat tatttcctac cgcaacaata cattcgtagt gcgcgcttct 11220 ggaggagatt taaatttggg aggaagggat gttgacagag cctttctgac tcatcttttt 11280 tctctaacat ccttagaacc cgatctttcg ttagacattt caaatctaaa agagtcgttg 11340 tctaaaactg acgcggaaat agtttacacg ctaaaaggga gcgacgataa aagggaggat 11400 gttagagtga ataaaagtat tctcactact gttatgctcc cttacgtcaa tagaacttta 11460 aacattttgg aagcaacact taaatcgtac gcaaaaaata tgggtgggtt acggagcgtt 11520 aaatgcgact tagtgctaat tggtggttct tcgtatcttc cgggtttaga agatatctta 11580 tcgaaacacc gacggatcga tcgaatattg aaagtcgctg atccccgagc ggcggtcgcg 11640 gtcggttgtg cgctatattc gtcgtgcctt tcaggttcac aagggctgtt attagtcgat 11700 tgcgcggctc acaccatcgc aatagccggt atgagctgtg accagattat ttgcgctcca 11760 gctggagctc ctattccctt ctcggggacc acaccgttgt acttaccaaa agctaataga 11820 aattcacaac gtcaaatcgc agtattcgaa ggtgaatacg tgaagtgtcc cagaaataga 11880 aaaatttgcg gttcaaacat taaatttctt gatatcggtg tcacaagcgt ttcacactca 11940 cccatcactt tctatttaga cgtagccata tctagtgtgg gggctatctc gtttgtggtg 12000 agaggtcccg aaggaaagga agtttccttg acgggcactc ccgcttacaa cttttcttct 12060 gtagtactcg gaacacgtag tgtccgagag ttgcatatta gtctaagcaa taaagttcat 12120 ctcggcctac ttctacaccg gaaggctgac cggcggttac tattttccga aggtgaagcg 12180 gttcgctacg tggaaacagt agaagttgcg gatgtgctaa aagaatttaa gagctacaac 12240 gctagttctc taccaccaga cgaagatgtc gaaccactca tggggaagtc tgttcaaaaa 12300 gtactacggg gaggcagatt ggaggaaata cctctctaag agcattgtag cccacacagc 12360 taacataaaa actttgccgg acgttcagtt gtacggcggt agaactgtga agaagtccga 12420 atttgaatcg gtgactccga ggtcgttcga gcaggaatta ggacttctga tgttaagtga 12480 acgggacgtg ggttggtgta agatgtgcgg gataactgtg gaggaggcaa tgtaccaaat 12540 cacgaaccct gaagcgtatc cgatcacagc ggaaaaatgc gatccagatg tgaaaactga 12600 ggggcagaaa tacaccatgg aagacgtgat gaatttcatg cgtcgttcca acctagatat 12660 aaatgataaa atcttagcgg aacagtgttg gtctctatcc aattcgtgcg gagttttgat 12720 taaccccgat gataaagaac ggtttgttgc tctcactttt aagaataacg aattggttaa 12780 cgattcaagc aacgcgaaca tagactgtcg cgtgggtgat tacttagttt acgctatgtc 12840 cctattcaat tgtaggagtc agaagtcgca agcgggtaac atttcgctct acgaaaagta 12900 ttgcgcttac atcagaactt acttagaaaa taccgatttg tatttcactt ctcccgatag 12960 gataccgtta ctcaccggca ttctctatga tttctgcaag gagtataata tcttttattc 13020 cacatacaaa cgaaacgttg ataatttcag gttcttccta acgaattata tgcccttgat 13080 ttcagacata ttcgttttcc agtgggtaaa accggcaccg gacgtgagat tactattcga 13140 tataagcgca gccgaattga ctttagaagt tccaacgtta agcttagtgg attcgcaggt 13200 ggtgatagga catgtcctga gatttgtcga atcatacact gctgatccgg ctattgacgc 13260 gttggaagag aaattggacg ctataatgaa gaagagtaat cctcatctat ctacggcgca 13320 actgtgggta ggattcttct gctattacgg cgaatttcgc accgcacaac gtcgggtggt 13380 gcaaaggccg ggtgtgtata agacgcccaa ttcggtcgga ggtttcgaga ttaatatgaa 13440 aaacgtcgaa gagttttttg ataagatcca acgagaggtg ccaaacgtgt ctctgcggcg 13500 acaattcaac ggagccagag cgcacgaggc attcaaagta tttaaaaagg gaaacatcag 13560 cttcaaaccc atatctcggt taaacatacc gagggagttc tggtatctga atgtggatta 13620 ttttagacat gcgaacagaa gcggactgag cgaagaggag gtgctcattc ttaataatat 13680 aagcgtagat gttcgtaaat tgtgtgcgga aagagcgtgc agcactctgc ctagcgcgaa 13740 gcgctttagc aaaggtcata aaagtagtgt gccatcattg cgtcaagaga aaaattatag 13800 agatccgttg atcacgttaa gaaactcttt atatgagttc cggcacggga atcgcgatag 13860 gagggtctga ggccatcgag acctcaggtt tcgtgctatc tacgcgaaag gaagccgtcg 13920 ataagttctt caatgaacta aggaacgaaa attattcttc ggtcgacagc agtcggttaa 13980 gcgaccaaga ggtgaaagaa gttctggaga aaagtaaaga gagctttaaa agcgaattag 14040 cgtccaccga cgaacacttc gtttatcaca tcatcttctt tctgattcga tgcgccatta 14100 tatccacgag cgataaggta aaatacgtag ggagccataa gtacgtagtc gaaggaaaga 14160 cgtacacagt tcaagacgtt tgggttttta acatgattaa ggggctgacc aagcagtaca 14220 aaagggtgaa tggtttgcgt gctttctgtt gtgcctgtga agatttgtat ctgacagttg 14280 ctcccgcgat gtctgaacgc ttcaaaacca aggctatcgg tatgaagggc ctacccgtcg 14340 gaaaggagta tttaggtgct gactttctct ctggaacgag tagattgatg agtgatcacg 14400 atagagctgt atccatagtc gcggccaaga acgctgtcga tcgtagcgca tttaccggtg 14460 gagagaggaa gatagttagt ttgtatgact taggaaggaa ctaagcacgt tgtgctatag 14520 tgcgcgtcat aatattaagt actagtgcat attaacaagc agtagtgttt aaagtgtagt 14580 gataagaaac aatggagttg atgtccgatg ataacttgag cggtctcgtc ataaccgacg 14640 cttctagttt gaatggtgcc gataagaaac tgctgtctgc ggaggtcata aaaatgcttg 14700 tgcagaaggg ggcgcccagc gaaggtattg aaatagtctt tgggctactg ctctacgctc 14760 tcgcagcgag aaccacatcg cccaaagttc agagggctga ttctgatata atctttcaaa 14820 acacttacgg tgacaagact gttgtcgtga cagaaggtga tctcaagaaa gtgcttgaag 14880 ggtgcgcacc tcttaataga ttcactaaca agccgcgaac tttcggccgc acgtttacgg 14940 aggcgtatat tgatttctgc gtggcttaca agcataagat gcctcagctc aacgcggccg 15000 caaatttggg gataccagca gaagattcgt acctagctgc ggacttttta ggtgcttgcc 15060 cgaaactctc tgaattacaa caaagtagaa aaatgtttgc aagtatgtac gctttgaaga 15120 ctgaggacgg agtagtaaat acgcccgtta gtaatttgcg ccaattaggt aggagagaag 15180 ttatatgatg gaaatttgcg ctcagaatcc tgaggcacta gtgctattac gcacaaatca 15240 aaacacaaca ttactagtcg tcaagtctga tgatgaagtt aatctgccga aattgttgat 15300 atgcgggtat ttacgcgtgt cgcatcgtgg ggatgtgact ggttgtaatc gcgaagacat 15360 aataaaggat tttgaaggtg cacatcatac cgtcatccgc tcgagaacgg tacagtatga 15420 ttacgagtcc gcagttaaag agtacaataa cgctgattgc gtggtgaaat tctttttaga 15480 aacaggagac gttttttggt tttttctcca aagtgacatc aaaggaagag cggcgcgaca 15540 cttgcgcacc ttcttcgaag ctaacaattt cttctttgga tcgcattgcg gtaccatgga 15600 gtattgtttg aagcaggtac tgatagaaac tgagtctgta atcgaatctt tttgtgaaga 15660 aagaaatcgt taagatgagg gtaatagtat ctccttatga agctgaagac attctaacaa 15720 gatcaactga aatgttgcga aacatagaca gtggagcgtt ggatacgaaa gagtgcattg 15780 aagcgttctc gacgctaacg cgagatttgc actgcgcaaa agcatcgtac caatggtgcg 15840 ttgacacagg gttgtgccga cgtgattgcg ccgaaaaacg cttgatcgac accgttgaat 15900 caaatctgag actcgctcaa cctctcatac gcgagaaggt ggcgactcat ttttgcaaag 15960 atgaacccaa agaactagtg gcgttcatca cgcggaaata cgttaagctc acaggtgtgg 16020 cggcgcggga ggctgtaaaa atagaaatgc gcactctatc taagacagtt ttaaataaac 16080 tatctttaga aatggccttc tatatatcac ctcgagcgtg gaaaaacgca gaatggttag 16140 atctcaggta tacgcctcta aaaatataca gagatctttt actagacgtg gacacactca 16200 acgccttatg cgaggaggat gaagttcacg tcgtcgtgac caataaagat gaagacgaga 16260 atcatgatct cgagcttcaa gaagactgtt aaagtctaaa attctagaat cgcaatatgt 16320 ttgcgagtag taaataaaat cgaacgtggg tggatctacc tgacgtatca actttagctg 16380 ttactgggta attaagctaa caagtgttag cgtaatgtgt atgttgatga agagaaaata 16440 ccgtttctag atcggtgact tttctcttct tttattttta aaataaaaa 16489 //