ID   JQ023131; SV 1; linear; genomic RNA; STD; VRL; 18659 BP.
XX
AC   JQ023131;
XX
DT   21-DEC-2011 (Rel. 111, Created)
DT   21-DEC-2011 (Rel. 111, Last updated, Version 1)
XX
DE   Grapevine leafroll-associated virus 1 isolate 1050, complete genome.
XX
KW   .
XX
OS   Grapevine leafroll-associated virus 1
OC   Viruses; Riboviria; Closteroviridae; Ampelovirus.
XX
RN   [1]
RP   1-18659
RA   Rott M.E., Belton M.;
RT   "Sequence variability within a single isolate of Grapevine
RT   leafroll-associated virus 1";
RL   Unpublished.
XX
RN   [2]
RP   1-18659
RA   Rott M.E., Belton M.;
RT   ;
RL   Submitted (09-NOV-2011) to the INSDC.
RL   Sidney Laboratory, Canadian Food Inspection Agency, 8801 East Saanich Rd,
RL   Sidney, British Columbia V8L 1H3, Canada
XX
DR   MD5; ea469d947a48dd095e2ff0a872c2a8ce.
DR   EuropePMC; PMC3889241; 24426308.
XX
CC   ##Assembly-Data-START##
CC   Assembly Method       :: CLCBio genomic workbench v. 4.8
CC   Sequencing Technology :: Illumina HiSeq
CC   ##Assembly-Data-END##
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..18659
FT                   /organism="Grapevine leafroll-associated virus 1"
FT                   /isolate="1050"
FT                   /mol_type="genomic RNA"
FT                   /country="Canada"
FT                   /isolation_source="infected plant 1050"
FT                   /collection_date="22-Oct-2010"
FT                   /db_xref="taxon:47985"
FT   5'UTR           1..781
FT   variation       207
FT                   /replace="c"
FT                   /frequency=".023"
FT   variation       376
FT                   /replace="c"
FT                   /frequency=".426"
FT   variation       458
FT                   /replace="t"
FT                   /frequency=".379"
FT   gene            782..7396
FT                   /gene="MET/HEL"
FT   CDS             782..7396
FT                   /codon_start=1
FT                   /gene="MET/HEL"
FT                   /product="methyl transferase/helicase"
FT                   /db_xref="GOA:G9L8E6"
FT                   /db_xref="InterPro:IPR002588"
FT                   /db_xref="InterPro:IPR005123"
FT                   /db_xref="InterPro:IPR027351"
FT                   /db_xref="InterPro:IPR027450"
FT                   /db_xref="InterPro:IPR037151"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8E6"
FT                   /protein_id="AEW24401.1"
FT                   /translation="MAVKSIFAQEDLISIQKDFRFPFVSSSTSLFLCNLPSPPIIFQKG
FT                   SRFIAPTRSSNGFLRKAIDYAVCCKNYGRSLKFDATPVQSTHHIIVKAVFRNTYYRAAS
FT                   GKWVTLRAGTASGTVRLVRPAVVELESDHAGMFSVFFVRKQLSEAPEKAKSPQCVIDRK
FT                   PAQSKIPVPTPIKAKQVAAAVPGLNGNNAQSHARFGKFFNEGGSFQRPAFLLRSGRLWD
FT                   IQGQCFFTPKFCHKWLLTRDWSSGKRYVFCFVEGAVIAVPLEVIRYDMRVLWRRLPVGV
FT                   YVALPHLLFRCEDTGELFYGDKYWCWLQLAVLNGNNLLAGSFESCISVRKLKRMLRFNV
FT                   KLEKTDEANIFHVGNKPTVSLAEVDDRCFVGMAAKGGQQSLVASVSNALNQEDLFEGIV
FT                   STIANRLVLKEGSTLVTHLDEKISELFMMKEDTLGKKNKCVVTVALSAVAKESLTRAFP
FT                   ELFITFLDSVSSSHSLCNAVRSCFNSLYASKYRGVPFVDIGGSVAYHVRNGDKDCHCCN
FT                   PVLDYKDCRRREEECLRLATAEEKVMTVESVLKKEAAKNITYCQMDTRFCDHKAPVGFM
FT                   VDVYDLDVFELAQALEKKDIKIFELCLMFPIELTARDGSLTIPELGVEVVRKGDVIMYT
FT                   VGGVGDAYTHSVQKIISFFGSNVVQLPSGSAFSVEYVGYRLGYHQFSLCVIDTAHASYN
FT                   LTRKVSTTFKGHSLVMIPEIADGFLSFRQMYLDSDFVDRVYSYLLNTTSAFVDRTFEYA
FT                   VSCARSQKTHVIVGTRVVHDKVELSPEEQWGLVVALMIQAITDRKKAHVAHHSIEALRG
FT                   NLWNCIVLIVRKLCSSFFSGMNEYALTMLQALGSNLDVLMDQNFRFVRTVPATVTLHLV
FT                   AETNPCFTDRSMLLDEGLMAYRLHLRNGTNIKVAKDLVREAHKILRSMRAAEKAKAESK
FT                   GLEVDEREITEDDILTFIQTKNLHVGLKGGVRSRIINQGVKSYVNSAASTFTSSGNPAS
FT                   WAKSFAELKVAENGYTVGKIFSKSPANGVVKLPIAATVASLVMKDDGGLGEKIEVGQPA
FT                   WVSLLASSLRALSERAAYVLRSKSSLLMFGGGLLLMASMLAFRRQILNRAHGLAQGVRT
FT                   SLKKTKGKLVLGLSDLKERLGSVRRNAVVSAQGSVRSLKVVARAEFKDRVSGYVEEMGY
FT                   IYGDSLNVQTVCLTAAAYLSSSYEKSAILILLAPKRFRYIGSVGYGIAALRCIKGGEYG
FT                   RASKLMGVAACLALTAKKGASRAASLQIVFPKRGDVYEGVTSFYRQRAERKGAAIEPSR
FT                   RSEGEAMDDILDEATCGTSSEVEQEKEDLTSKTEDSTDASDDESVESSQHTAQSHFVTT
FT                   SVNESKHESGILADGKVESRKEEEPRRIVDKGKKVVDYDEELNMRKEKCRLIIEKRREE
FT                   HGGLIPVVFKEPKCEGKDDESEEESEGYLCLSSTKVERGDASPSGCKSADCKSISEESP
FT                   ERKTKSSAIIEDVTPVKVEVSAPRNVQTAMEREKFKGARKLVSSDIFVDKLRGRDVAFY
FT                   SKYSRRYVYNGGSHASQGWNKALDELREELKLDESYDHCLIQKYRKGATIGFHADDEKC
FT                   YTSGVSVVTVNLNGQARFRVRSNKTGEIVEHLLGDGDVFVMSPGMQRDFKHSVESLDEG
FT                   RVSITLRNATVDYCKREATEGKRVKGEVTPQSRVSVNDGKTSKEAKGLLGPVPSSGVSS
FT                   SSGESVATVRQSASKSTIGTAVEMGCIDKDSWVLGEDPVLGIQSFNDVVYCKDLKTAGT
FT                   EAPAIEFIMYLARRCFDLFIKLKRARDLVAKTDKRVGHKFPLAVYENLPGLRVYDANFK
FT                   VVANAPEDGTTIFDLEYVFLVSTGTFVPGRNLEAVLSRQDAVLVCDDLLVFHDAMNLRG
FT                   CVRLAKRALVGEYMKNVRLSAVNSPPGGGKTTRLVDEYFGRKKRAKIATANTGSAADIN
FT                   SAIRAREGKKETDLVAKTANSWIVNSRPRPNSHVGLIDEVYMLHKGMFQLTVVAMGVKE
FT                   VIAYGDKNQIPFINREKTFVTPNEAVEFAEELIEYTDISYRCPADVCYVLSSMTDMRGA
FT                   KLYPNGVFPNGDTRPLRSFEKVPIATPEDALLYEADVYLTMTQNEKAEMQRVVAKMEIA
FT                   AGKKRPDVLTTHEAQGKTYGDVVLVRLKKADDPIFSRKPHIVVALSRHTRSMKYAVLSS
FT                   KMSDTISKLIDGTYAGKVSDVLLQQLQRNDRFRVD"
FT   variation       1086
FT                   /replace="t"
FT                   /frequency=".013"
FT                   /note="results in alanine to valine substitution"
FT   variation       1335
FT                   /replace="t"
FT                   /frequency=".011"
FT                   /note="results in proline to leucine substitution"
FT   variation       1381
FT                   /replace="t"
FT                   /frequency=".014"
FT                   /note="results in lysine to asparagine substitution"
FT   variation       1762
FT                   /replace="c"
FT                   /frequency=".023"
FT   variation       2084
FT                   /replace="g"
FT                   /frequency=".012"
FT                   /note="results in lysine to glutamic acid substitution"
FT   variation       3004
FT                   /replace="a"
FT                   /frequency=".019"
FT   variation       3133
FT                   /replace="g"
FT                   /frequency=".022"
FT   variation       3148
FT                   /replace="a"
FT                   /frequency=".064"
FT   variation       3157
FT                   /replace="g"
FT                   /frequency=".050"
FT   variation       3162
FT                   /replace="c"
FT                   /frequency=".045"
FT                   /note="results in isoleucine to threonine substitution"
FT   variation       3166
FT                   /replace="c"
FT                   /frequency=".038"
FT   variation       3899
FT                   /replace="a"
FT                   /frequency=".034"
FT                   /note="results in glutamic acid to lysine substitution"
FT   variation       3983
FT                   /replace="t"
FT                   /frequency=".381"
FT   variation       4018
FT                   /replace="g"
FT                   /frequency=".019"
FT   variation       4166
FT                   /replace="g"
FT                   /frequency=".011"
FT                   /note="results in serine to glycine substitution"
FT   variation       4560
FT                   /replace="g"
FT                   /frequency=".015"
FT                   /note="results in aspartic acid to glycine substitution"
FT   variation       4887
FT                   /replace="a"
FT                   /frequency=".035"
FT                   /note="results in arginine to lysine substitution"
FT   variation       5031
FT                   /replace="g"
FT                   /frequency=".011"
FT                   /note="results in glutamic acid to glycine substitution"
FT   variation       5053
FT                   /replace="t"
FT                   /frequency=".026"
FT                   /note="results in glutamic acid to aspartic acid
FT                   substitution"
FT   variation       5305
FT                   /replace="t"
FT                   /frequency=".010"
FT   variation       5696
FT                   /replace="g"
FT                   /frequency=".016"
FT                   /note="results in arginine to glycine substitution"
FT   variation       5716
FT                   /replace="t"
FT                   /frequency=".020"
FT   variation       5776
FT                   /replace="c"
FT                   /frequency=".067"
FT                   /note="results in glutamic acid to aspartic acid
FT                   substitution"
FT   variation       6748
FT                   /replace="g"
FT                   /frequency=".011"
FT   variation       6874
FT                   /replace="g"
FT                   /frequency=".029"
FT   variation       7123
FT                   /replace="a"
FT                   /frequency=".012"
FT   variation       7141
FT                   /replace="a"
FT                   /frequency=".460"
FT   variation       7142
FT                   /replace="g"
FT                   /frequency=".027"
FT                   /note="results in threonine to alanine substitution"
FT   variation       7297
FT                   /replace="c"
FT                   /frequency=".027"
FT   variation       7309
FT                   /replace="a"
FT                   /frequency=".010"
FT   gene            7374..8927
FT                   /gene="POL"
FT   CDS             7374..8927
FT                   /codon_start=1
FT                   /gene="POL"
FT                   /product="RNA dependent RNA polymerase"
FT                   /db_xref="GOA:G9L8E7"
FT                   /db_xref="InterPro:IPR001788"
FT                   /db_xref="InterPro:IPR007094"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8E7"
FT                   /protein_id="AEW24402.1"
FT                   /translation="MIAFESIECFSAAPQLEKYIMAKVSTSHVQVVNLFLESRVPGITC
FT                   FNTEFWEDDFETSGLDTLVETATISDNFPKYRVTDRPRLQNVVRSVIGRPKRNSLKCNL
FT                   VTFESRNFNADRGCDVSSDPIAATELASLFFDTWVDGSKLAECVGDTISQNAVAASSWL
FT                   DSRSSMAKQSLWARLRSFVYDLSAMTRYKLMVKSDAKPKLDSTPLQQYITGQNIVYHDR
FT                   AITAMFSHIFTQAVERLKYVLHTKVLAYHGMSTEDFSREVGDRLGDVSGYYVYELDISK
FT                   YDKSQGACMKDVERRILIGLGVSESVVDAFFCGEYDSVVTMGKNELVLSVGAQRRSGGA
FT                   NTWLGNTLVLMTLLAISLKGSEPSLVVVCGDDSLIFSKNELQIDDRILEESFGFDLKLT
FT                   CQCAPYFCSKYLVRTPDHCYFLPDPFKLFLKLSLEREPNEDLLFEVFTSFVDLTRGFGD
FT                   ESAVQQLVAMDAQRYGPNPYAYAAFCLVHILAANFVQFKRLFYDADGVFGKNSKSKATR
FT                   "
FT   variation       7528
FT                   /replace="g"
FT                   /frequency=".044"
FT                   /note="results in glutamic acid to glycine substitution"
FT   variation       8007
FT                   /replace="g"
FT                   /frequency=".444"
FT                   /note="results in isoleucine to valine substitution"
FT   variation       8160
FT                   /replace="a"
FT                   /frequency=".026"
FT                   /note="results in glycine to serine substitution"
FT   variation       8980
FT                   /replace="g"
FT                   /frequency=".034"
FT   variation       9046
FT                   /replace="a"
FT                   /frequency=".458"
FT   variation       9047
FT                   /replace="c"
FT                   /frequency=".456"
FT   variation       9065
FT                   /replace="t"
FT                   /frequency=".481"
FT   variation       9072
FT                   /replace="a"
FT                   /frequency=".500"
FT   variation       9087
FT                   /replace="a"
FT                   /frequency=".453"
FT   variation       9098
FT                   /replace="a"
FT                   /frequency=".477"
FT   variation       9112
FT                   /replace="t"
FT                   /frequency=".462"
FT   variation       9120
FT                   /replace="c"
FT                   /frequency=".446"
FT   variation       9121
FT                   /replace="t"
FT                   /frequency=".013"
FT   variation       9126
FT                   /replace="g"
FT                   /frequency=".419"
FT   variation       9128
FT                   /replace="a"
FT                   /frequency=".423"
FT   variation       9133
FT                   /replace="t"
FT                   /frequency=".397"
FT   variation       9135
FT                   /replace="t"
FT                   /frequency=".397"
FT   variation       9145
FT                   /replace="g"
FT                   /frequency=".383"
FT   variation       9471
FT                   /replace="g"
FT                   /frequency=".020"
FT   variation       9619
FT                   /replace="a"
FT                   /frequency=".059"
FT   CDS             9680..9859
FT                   /codon_start=1
FT                   /product="p7"
FT                   /note="ORF2"
FT                   /db_xref="GOA:G9L8E8"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8E8"
FT                   /protein_id="AEW24403.1"
FT                   /translation="MDLRQFSHELLYTVSLFILVALCFVVYFIVRAIRHCCAKIEDKPV
FT                   REPGNSSYRYVAQP"
FT   gene            9865..11496
FT                   /gene="HSP70"
FT   CDS             9865..11496
FT                   /codon_start=1
FT                   /gene="HSP70"
FT                   /product="hsp70-like protein"
FT                   /db_xref="GOA:G9L8E9"
FT                   /db_xref="InterPro:IPR013126"
FT                   /db_xref="InterPro:IPR029047"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8E9"
FT                   /protein_id="AEW24404.1"
FT                   /translation="MEVGLDFGTTFSTACFSVPSEDDSGCVTLVNSPFVPTQVFIGSDM
FT                   TYSIGHRAYSDFVAGKPGGLYINPKRWVGVDGYNFQAVKRKLNPEYEVKLSNGEVVLGS
FT                   VGNVNAPLVRVVDLVFLFVKGILLETEEAVGKAVSGVVCTVPAEYNSFKRSFLGVALEG
FT                   LGKPLRALINEPTSAALYGAVRGGALKETYAVFDFGGGTLDISFISRFNNVVSVLFSKG
FT                   DNFLGGRDIDRAIVQFLRKEKRITGVIDAGILAVMIADLKEKICVNGGTQYTQVKTSNG
FT                   LETLSMSVDELNAVSEPFVDRAIKIFAEGANELKRSPIVCVLTGGSVALPLVQQKLKTL
FT                   PYVSRTEYDSKTFRLSVAVGAKIYSDILTGHSDLRLIDTVSQTLSDELSGFTEIVIFPK
FT                   GHPIPSEYETSFQISGSTMEYGIIEGESNRTWLNEIAFKGTDYRPSNERKSDKVKYEIS
FT                   VDGKLKLSVDGRQLKNTRLPAPVSASAHAYRYVSSMKKYLIQLENNYVDMFSELHGDKI
FT                   SIDDVYDATGAYFDKNILVNFLRLSK"
FT   variation       9876
FT                   /replace="g"
FT                   /frequency=".016"
FT   variation       10131
FT                   /replace="g"
FT                   /frequency=".010"
FT   variation       10422
FT                   /replace="g"
FT                   /frequency=".024"
FT   variation       10699
FT                   /replace="g"
FT                   /frequency=".011"
FT                   /note="results in serine to alanaine substitution"
FT   CDS             11493..12941
FT                   /codon_start=1
FT                   /product="p55"
FT                   /note="ORF4"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F0"
FT                   /protein_id="AEW24405.1"
FT                   /translation="MNPLVWLTGATDYMSVLRPLYEDFKAGGGRRVVNYIEVIDRGDKP
FT                   HIIRGLSPSDENFELIAWAVAVGAFENSIYAFDTILSTMRNWKAYATPGSLQLDTPITK
FT                   KKNKHFTLTAEQQAAVRTSPNSTNEAFAQLFLNLGVFLKRVPKDEDISGRGNIPLVRVS
FT                   QAESTDRIEQGRYSPLLAKFVRKYTHVVDAYDSVVTRQKIEVCSYYIPELIGLLFRARD
FT                   GAQAYKTVSLYLASFLQSKDPGKYDPETRVIWFARTLTMARELLEIYYNIRVEVSEREI
FT                   IRNFRIQPGDEFHQLSTMLLGLVADTSAKLTGITDVDVVLRRVLGLCLKAMVDENQHIS
FT                   NINYTGALYLMLVYFSIVGTNYLRKKDTPNEMVFRINGVVRKVNFTHLKRLVAEASVDG
FT                   RNYPREISSVFAGITLQLRQLGNIDTVVWPDVVLSNPSLGFDTVLHSGYISLNPAMYKD
FT                   IEIIKRKIRNSTHWVGGYKYGKRF"
FT   variation       11685
FT                   /replace="t"
FT                   /frequency=".017"
FT                   /note="results in valine to leucine substitution"
FT   variation       12280
FT                   /replace="c"
FT                   /frequency=".106"
FT                   /note="results in arginine to proline substitution"
FT   gene            13046..14014
FT                   /gene="CP"
FT   CDS             13046..14014
FT                   /codon_start=1
FT                   /gene="CP"
FT                   /product="coat protein"
FT                   /db_xref="GOA:G9L8F1"
FT                   /db_xref="InterPro:IPR002679"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F1"
FT                   /protein_id="AEW24406.1"
FT                   /translation="MASVISQNDDDYNVVRGGNIVVPRTPTLNGFGTSAFTIPAGEATA
FT                   YVLKTQYSKPEAGTPEANHPVIGVLPDEYVFVKGPGGYTLRPSRPSTGQRSGDSEVAKR
FT                   EMGDKLKRTFNIAEIFTNPEMNIIFEPPKDMEVSVVVPTGPGLVTPAVATAISTELKNL
FT                   CAEVMGNTDQKSLTDFFLAMLQLMLTFSTSPDTESKEEYFVNLYSNGERKLTYEKVKGA
FT                   VVKGAEGSTFENPMRQYARLFSATAVHLILNGKLRPNEKVAMQHGVPKRFLPYTFDFCR
FT                   PSYSQFSNDAIRAWQLAAESAFGRKSNVTSSVLRNTSELKV"
FT   variation       13274
FT                   /replace="t"
FT                   /frequency=".060"
FT                   /note="results in valine to leucine substitution"
FT   variation       13675
FT                   /replace="t"
FT                   /frequency=".018"
FT   gene            14037..15539
FT                   /gene="CPd1"
FT   CDS             14037..15539
FT                   /codon_start=1
FT                   /gene="CPd1"
FT                   /product="coat protein duplicate 1"
FT                   /db_xref="GOA:G9L8F2"
FT                   /db_xref="InterPro:IPR002679"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F2"
FT                   /protein_id="AEW24407.1"
FT                   /translation="MTTALVAPIVGDKGEYLSLLRNLPNDNSQESWLPNAGRMNDDFFK
FT                   IGEAYKLQNLSIGYTINVSVSIKSIPKETMGVLRVIIEPRDGDMLIHTLRFYRDKNLIE
FT                   QVYTQRKGGVGSVLKSRPVDSSIVNFHNERKETMTSMVNKQNRLMEVFTNGSSLGSSQI
FT                   KVPVDSLITVIVSLESKKVSCDGLSELVPNDVGSIVKFDGTVDYKEINRERKYIFPYKT
FT                   FRDSKYREILTEAIAARNETAAAQPETETVPVHPIANVTYVTPPEGVSITNAAIPETKP
FT                   IDEVATLLTAIENVTDLPIGRSKLNVSELDVIELTGYYNPSVMEADDVRSVNAKLLDYY
FT                   SRLYGRSESSNRALAIGMIQGALTWSTSANLKDGEIRKVDVSLNGRKFTVDFNELRHII
FT                   QSSVPQSKYENPVRQYMRWFSTTTISLIKSGVVVPNYHVMARHGVTSQFIPYGFDYCIL
FT                   LPSYNRRDDKVAAALARAQAIALANKRRAGKTLYNFSELEKS"
FT   variation       14060
FT                   /replace="c"
FT                   /frequency=".014"
FT   variation       14402
FT                   /replace="a"
FT                   /frequency=".207"
FT   variation       14502
FT                   /replace="a"
FT                   /frequency=".329"
FT                   /note="results in serine to threonine substitution"
FT   variation       15096
FT                   /replace="t"
FT                   /frequency=".026"
FT   variation       15358
FT                   /replace="c"
FT                   /frequency=".025"
FT                   /note="results in arginine to proline substitution"
FT   variation       15500
FT                   /replace="g"
FT                   /frequency=".010"
FT   gene            15540..16862
FT                   /gene="CPd2"
FT   CDS             15540..16862
FT                   /codon_start=1
FT                   /gene="CPd2"
FT                   /product="coat protein duplicate 2"
FT                   /db_xref="GOA:G9L8F3"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F3"
FT                   /protein_id="AEW24408.1"
FT                   /translation="MEIDEAVDSRNAIRIYDVKRALLSLSKSMVLRSRVRFVRRAGFRS
FT                   LYQVWIGRGKNVLELGVDFGGTHGAKLRPFVYYGGSYYYNEQMVPTDISEDVLDYVNVE
FT                   ISLTLSKKRVVTAALINGIDVPCLGYALKNGTLRFGHEIIFKVDDDSLHYLLDKSTRNK
FT                   RIEGLYTELVVKVDGGDVLLGDVDMAGTPLDRAYSNKTGIEPSIHVANVGDWKSLFVLS
FT                   ASDTRTGVEQPYTENAMLYYEVVDRNLEDVAKTCGYFEIPTATVAGLTMVDFEGMEKVT
FT                   LSVSGESTYLSKDVAKRITLALLRLPLTVDDGSVLTDKRLLLVLIIQGAVTYGTRADFV
FT                   EKGPSNVCVSYKGKSVRLDFMYVRSVIIANCDSIYITNPIRKYMRWWSTATIQLIKLGI
FT                   VKPNPIVAAKRGLSSNNIWLSFDYILLDARFNDRKEKILLR"
FT   variation       15624
FT                   /replace="g"
FT                   /frequency=".019"
FT                   /note="results in methionine to valine substitution"
FT   variation       15753
FT                   /replace="t"
FT                   /frequency=".025"
FT   variation       15892
FT                   /replace="g"
FT                   /frequency=".018"
FT                   /note="results in alanine to glycine substitution"
FT   variation       16103
FT                   /replace="a"
FT                   /frequency=".012"
FT                   /note="results in methionine to isoleucine substitution"
FT   variation       16109
FT                   /replace="g"
FT                   /frequency=".304"
FT   variation       16119
FT                   /replace="a"
FT                   /frequency=".240"
FT                   /note="results in aspartic acid to asparagine substitution"
FT   variation       16136
FT                   /replace="t"
FT                   /frequency=".012"
FT   variation       16368
FT                   /replace="g"
FT                   /frequency=".012"
FT                   /note="results in methionine to valine substitution"
FT   variation       16479
FT                   /replace="g"
FT                   /frequency=".018"
FT                   /note="results in serine to glycine substitution"
FT   CDS             16946..17515
FT                   /codon_start=1
FT                   /product="p21"
FT                   /note="ORF8"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F4"
FT                   /protein_id="AEW24409.1"
FT                   /translation="MEFAPVCYICTKNEEGSDKKLTLLSREDLIDFNSDENQDELSLEK
FT                   KPFVKSYENYLRDWAERPFYFSNAMSVMDLAMHTGNASIDSDSGLSKSGEKRRTAVEVQ
FT                   SPWKTGIPFDSVDNGVLVAVGYEGLNADKKKARSVALFMQNEGSYIMLIGGEVYASSSY
FT                   IKECMRCQRLASTMSGDVFVDALRLV"
FT   variation       17188
FT                   /replace="a"
FT                   /frequency=".054"
FT                   /note="results in asparagine to lysine substitution"
FT   variation       17484
FT                   /replace="g"
FT                   /frequency=".016"
FT                   /note="results in aspartic acid to glycine substitution"
FT   CDS             17559..18191
FT                   /codon_start=1
FT                   /product="p24"
FT                   /note="ORF9"
FT                   /db_xref="UniProtKB/TrEMBL:G9L8F5"
FT                   /protein_id="AEW24410.1"
FT                   /translation="MALITPKYVGNFSEFCVHIIEISNADSVHLLYEALKLNSRWLELS
FT                   FSTFVEETQKELEQTPNGWQLRFARENTVAEIDTKFMRKMYGMYLREADTLKDIRKALH
FT                   LILVDTKLTDASVNGLARGRGKKNFSRSLLKGMMRAIRELQSSLDKIGVKSGAEVKLTA
FT                   IAISLLAEDSHSSPKDVILRVNRLSSYYLLKDNVTFHFFGAIASNLV"
FT   variation       17860
FT                   /replace="g"
FT                   /frequency=".024"
FT                   /note="results in lysine to arginine substitution"
FT   variation       18002
FT                   /replace="g"
FT                   /frequency=".030"
FT   3'UTR           18192..18659
FT   variation       18464
FT                   /replace="a"
FT                   /frequency=".067"
XX
SQ   Sequence 18659 BP; 5015 A; 3439 C; 4946 G; 5259 T; 0 other;
     gtaggtatcg aaccagacaa tcccaatcct accattgctt tccatttcag atctgaaatc        60
     cacacttctt ctcttctctc cgcttccgag cattcttact ctcattcctt tttctctcga       120
     gtgctaaaac cactcccttc tcactaccct tttacctctc ccttttcgtt tcctttctct       180
     cagggacgaa gtatatactt cacctctcta ctgttctact tcttctcctt ttctttcctt       240
     tttctcttga gtgctgatat cactctattc tcactacttt ttcacttctc cctttttgtt       300
     tcctttcatt cagggacgaa gcacgtactt cttctcttta ctcttctccc ttttctcctt       360
     atctttcctt tcttttttat tttttattta actctattta tccgccttct ttcttttcgt       420
     atcgttcatt tccttctctt ttctttttct ttttatcgtt actttttcgc cttctttctt       480
     attgtatcca tcgtttcctt ttcttttctc cttagttcgt cactctaacg ttttcccgaa       540
     ccactccatt tcttttcatt tcctttcatt tcgcgtgctt cactttattg tttccccttt       600
     cttttactgt ctaggttcta atttttcctt ttcctcctta gtattttgca gtatcggttt       660
     catttctaac ttttttattc gtcttgtact tccttttatc ttcgcttttc tcgtttttgt       720
     cgttccccat tgttctcttt tcttattttt atatttcctt tcttctcgtt tatttaaaat       780
     catggcagtt aagtccatct ttgcacaaga agacttgatt tccattcaaa aagattttcg       840
     tttccccttt gtttcttctt ctacatcttt atttttgtgc aatcttcctt cacccccaat       900
     catatttcaa aaagggtcac gctttatagc ccccacaagg agtagcaatg gttttctgag       960
     gaaagcgatc gactatgctg tttgttgcaa aaactacggt cggtcattga aatttgacgc      1020
     tacacctgtc cagtccactc accacataat agtaaaggct gtgttcagga acacatatta      1080
     tagggctgcg tcaggcaagt gggtaacttt gcgggcaggg acagcttcag gtacggtacg      1140
     tttagtgagg ccggcggtcg tggaacttga atcagaccac gccgggatgt tttctgtttt      1200
     cttcgtaaga aaacagctgt cggaggcacc agagaaggct aaatccccgc agtgtgtgat      1260
     cgaccgaaag ccagcgcagt cgaaaatacc ggtgcctaca ccaataaagg caaagcaggt      1320
     tgcggccgcc gtgccgggtc taaatgggaa caacgcgcaa tctcacgcgc gtttcggaaa      1380
     attttttaat gaaggcggtt cgtttcagcg cccggcattt ttgttgcgtt cggggcggct      1440
     atgggatatt caagggcaat gttttttcac cccgaaattt tgtcacaagt ggttgttgac      1500
     ccgcgactgg agttcaggta agaggtatgt tttttgtttc gttgaagggg cagttatcgc      1560
     cgtacctctt gaagttataa ggtacgatat gcgcgttcta tggcggaggc tgcctgtggg      1620
     tgtttacgtg gcgcttcctc acttgctctt tagatgtgag gacacgggtg agctgtttta      1680
     tggtgataag tattggtgtt ggttgcagct ggcagttctg aacggcaaca atcttttggc      1740
     cggctctttc gagagctgta ttagtgtgcg gaagttgaag cgaatgctgc gctttaacgt      1800
     caagctggag aagacggatg aagcgaatat ctttcatgtg gggaacaaac ccactgtttc      1860
     tttggctgag gttgatgata gatgcttcgt cggtatggcg gctaaagggg ggcaacagag      1920
     cctggtggca agcgtaagta atgcgctcaa tcaggaagat ctattcgaag ggatagtatc      1980
     aacaattgct aaccggctag tactgaaaga agggagcaca ctggtgacgc acctcgacga      2040
     gaagatttcg gagctgttca tgatgaagga ggatacactg gggaagaaga ataagtgcgt      2100
     cgtgacggta gcgcttagtg ctgtggctaa agaaagtttg acgagagctt ttccagaact      2160
     ttttataacc tttttggata gtgtttcgag ctcgcacagt ttatgtaacg ctgttcgttc      2220
     gtgcttcaac tcattgtatg cgagcaaata cagaggcgta ccgttcgtag acatcggagg      2280
     gagtgttgcc tatcatgtgc gtaatggaga caaagattgt cattgttgta accccgttct      2340
     ggattacaaa gattgcagaa ggcgcgaaga ggagtgtttg aggctcgcta cagctgagga      2400
     aaaggttatg acagtcgaaa gcgtgcttaa gaaagaggct gcgaagaaca tcacgtactg      2460
     ccaaatggac acgcgctttt gtgatcataa agcgccagtt ggtttcatgg ttgacgtcta      2520
     cgacttagat gtttttgaat tggcgcaagc actagagaag aaagatataa aaatattcga      2580
     gttgtgcttg atgtttccaa tagagctgac agccagagac ggcagcctaa ctataccaga      2640
     actgggtgtg gaagtggtgc gaaaaggcga tgtgataatg tatactgtcg gaggcgtagg      2700
     cgatgcttac actcattccg tgcagaagat tataagtttc ttcgggagta acgtcgttca      2760
     gttacccagt ggcagtgctt tttcggtcga gtatgtaggc tacaggcttg gttaccatca      2820
     gttttctctg tgcgttatcg ataccgctca tgcctcgtac aatttgacga ggaaagtgtc      2880
     gacgacgttt aagggacata gtctggttat gataccggag attgctgatg gttttctctc      2940
     attcagacaa atgtacttag attccgattt cgttgatcgt gtgtactcgt atttgcttaa      3000
     tacgacgtct gcattcgtgg atagaacatt tgagtacgcc gtgtcttgcg cgcggagcca      3060
     gaagacgcat gttatcgtgg gtacacgtgt ggtgcacgac aaggtcgaat tgtctccgga      3120
     agagcagtgg ggactggttg tagcgttgat gatacaagcg atcacagaca gaaagaaggc      3180
     acacgtggct catcactcga tcgaggctct tcgcgggaac ttgtggaatt gtatcgtttt      3240
     gatcgtacgg aagctttgta gctctttctt ttcggggatg aatgagtacg ctttgactat      3300
     gttgcaggcg ctggggtcta atctggacgt gttgatggat caaaattttc ggttcgtacg      3360
     tacggttcct gccacggtga cgctacatct ggtggcggag accaaccctt gtttcactga      3420
     tagatcgatg ttattggatg agggtttgat ggcttacagg ttgcacctcc gtaacgggac      3480
     gaatatcaag gtcgctaagg atctagttcg tgaagcgcac aaaattctga ggagcatgag      3540
     agccgcagaa aaggcgaagg ctgagagcaa aggtttggag gttgatgaga gagaaatcac      3600
     tgaagatgat atcctgacgt ttatacagac gaaaaatctc catgtcggtt tgaaaggtgg      3660
     tgttagaagt cgcataatca atcagggcgt aaagtcatac gtgaattcag cggcgagcac      3720
     gtttacatcg agcgggaatc cggcaagttg ggccaaatct ttcgctgaat tgaaggtggc      3780
     ggagaacggt tacaccgtgg gaaaaatttt ttcaaaatct ccagcgaacg gagtagtcaa      3840
     gcttccaatt gcggcaacgg tggcatcgct ggtaatgaag gacgatggag gtctagggga      3900
     aaaaatagag gttgggcagc ctgcgtgggt atctttgctt gcgtcatcat tgcgcgctct      3960
     gagcgagagg gcggcttatg ttctacggag taagtcgtcg ttgctaatgt tcgggggagg      4020
     tctattactg atggcttcca tgctcgcttt tcggcgccaa attttgaaca gagctcatgg      4080
     gttagcgcaa ggtgtccgca cctccttgaa aaagacgaaa ggtaagctgg tgttaggtct      4140
     gagcgacttg aaagagagac tcgggagtgt aagaaggaat gccgtcgttt cggctcaggg      4200
     atcagtgcgt tcattgaagg tggtagcgag ggctgaattt aaagatcgcg tgtctggata      4260
     cgtggaagag atggggtaca tttacggcga ctcgttgaac gttcagaccg tgtgccttac      4320
     ggccgccgca tatctatcat cgagctatga gaaatcggcg attcttatat tactcgcgcc      4380
     taagcggttc aggtatatag ggagcgtcgg atacgggata gcggcattga ggtgtatcaa      4440
     aggaggcgag tatggcaggg cttcgaaatt gatgggcgta gccgcgtgtt tggcgttgac      4500
     tgcaaagaaa ggtgcaagta gggcagccag cttgcagatt gtgttcccga agcgtgggga      4560
     cgtgtatgag ggcgttacta gcttctacag gcagcgagca gaaaggaagg gtgcggcaat      4620
     cgaaccgtcg cgcagaagcg aaggcgaggc tatggatgat atcctggatg aagcaacttg      4680
     tggaacctcg agcgaagttg agcaagagaa ggaggatttg acgtcaaaaa ctgaggattc      4740
     aacagacgca tcagatgacg aatcagttga aagtagtcag catacggcac agtcgcactt      4800
     tgtgactaca agcgtcaacg agtcaaaaca cgaaagcggt atcttggctg atgggaaagt      4860
     cgaaagtcgg aaggaagagg agccgaggcg tattgtcgat aaagggaaga aggtcgttga      4920
     ttacgacgaa gagttgaata tgcggaagga gaagtgcagg ttgattatag agaagagacg      4980
     agaagagcat ggtggcctaa ttcctgttgt gtttaaagaa ccgaagtgtg aggggaagga      5040
     cgacgaatcg gaagaagaga gcgaaggcta tctgtgctta agctctacca aagtggagcg      5100
     tggcgacgct tcgcccagtg gttgtaagtc cgctgattgc aagagtatat cggaggaaag      5160
     cccggagaga aagacgaaat cgtcagcgat tatcgaagat gttactccgg tcaaggtcga      5220
     agtgtcagct ccccgaaacg ttcaaacggc gatggagcgt gaaaaattca aaggggcccg      5280
     caaactagtg agcagcgaca ttttcgtcga taagttgcgg gggagagatg tagcattcta      5340
     cagcaagtac agccgaaggt atgtctataa tggtggttcg cacgcgagtc agggttggaa      5400
     taaagcgctt gatgagcttc gggaggaact gaaattggat gagagttatg atcattgctt      5460
     gatacagaag tacagaaagg gtgctacgat cgggtttcat gctgatgatg agaaatgcta      5520
     cacatctggt gtgagcgttg tcaccgtaaa tttgaatggt caggctcgct ttagagtgcg      5580
     gtcgaataag acgggagaga ttgtcgagca tcttctgggt gatggtgatg tcttcgtgat      5640
     gagcccgggg atgcagcgtg attttaagca ttcggtggaa tctttggatg aagggagggt      5700
     gagtattaca ttacgcaacg ccactgtgga ttattgcaag cgcgaagcta ctgaaggcaa      5760
     gcgcgttaaa ggcgaagtta ctcctcaatc ccgggtatcg gtgaatgacg ggaagacgtc      5820
     gaaggaagct aaaggtctac tgggacctgt gccaagttca ggggttagtt cttcttcagg      5880
     cgaaagtgtg gcgaccgttc ggcaaagcgc gtcaaagtct actatcggca cagctgtgga      5940
     gatggggtgt atcgacaagg acagttgggt attaggtgaa gacccagtct tggggataca      6000
     atccttcaat gatgtcgtat actgcaaaga tctgaagacc gcgggtactg aagccccggc      6060
     gattgagttc attatgtatt tagcacggcg atgtttcgat ttgttcatca agctgaagcg      6120
     agcgcgagat cttgttgcta aaacggacaa aagagtagga cacaagtttc cgctcgcagt      6180
     ttacgagaat cttcctggtt tacgagtgta cgacgcgaat ttcaaagtgg ttgcaaatgc      6240
     gccggaagac gggacgacta tcttcgattt ggaatacgtc tttttggtat ccacaggcac      6300
     tttcgtgcca gggcgaaatc tcgaggcagt attgtcgagg caagatgccg tgctagtttg      6360
     tgatgatcta ctagtgtttc atgatgcgat gaatttgcgt ggatgtgtta ggttggctaa      6420
     gagggcgttg gtcggtgaat acatgaagaa tgtgaggttg tccgcggtga attcaccacc      6480
     tggtggaggc aagacgacgc gtctcgttga cgaatatttt ggaaggaaga agcgtgcgaa      6540
     gattgctaca gcgaacactg gaagcgctgc agatattaac tcggctattc gagccagaga      6600
     agggaagaag gagaccgatt tggtagcgaa gaccgcgaac tcgtggattg ttaattcccg      6660
     gccccgtcca aactcgcatg ttgggcttat cgatgaagtg tatatgctgc ataagggaat      6720
     gtttcagtta acagtggtag cgatgggagt gaaagaagtt atcgcgtacg gagataagaa      6780
     tcagatacca tttatcaaca gggaaaagac gtttgtcacg cctaatgaag ccgtagaatt      6840
     cgcggaagag ctgattgagt acactgacat ttcatacagg tgccctgctg atgtatgtta      6900
     cgtattatcg tcgatgactg atatgcgagg agctaaattg tacccgaatg gtgtgtttcc      6960
     taacggggac actcgacctt tacgctcttt cgagaaggtg cctatcgcaa ctccagagga      7020
     cgctttactg tatgaagcgg atgtttacct gacaatgact cagaatgaaa aagcagaaat      7080
     gcagagggtt gttgcgaaga tggaaattgc ggctggaaaa aagaggccag atgtgttaac      7140
     gacgcatgaa gcgcaaggca agacgtatgg tgatgttgta ttggtaagat tgaagaaggc      7200
     agatgatccg atcttctcgc ggaagcctca tattgtagtt gctttgtcaa ggcatactcg      7260
     cagcatgaaa tacgcggtgc tgagctctaa gatgtctgat actatctcga agctgattga      7320
     tggtacgtac gctggaaaag tgagtgacgt tttgttgcag caactgcagc ggaatgatcg      7380
     ctttcgagtc gattgagtgt ttctcggctg ctccgcagct ggagaagtat attatggcga      7440
     aggtttctac atcccacgtt caagtggtga atcttttctt ggagagcaga gtgcccggta      7500
     tcacgtgttt caatacggaa ttttgggagg atgattttga gacgtctggt cttgatacgt      7560
     tggtcgaaac ggccacgata agtgacaact tcccgaagta tagggtaacg gacaggcctc      7620
     ggttgcagaa tgtggtaagg tcagtgattg gacggcccaa gcggaacagt ttgaaatgta      7680
     acctcgtcac gtttgagagc agaaacttca atgcagacag gggatgtgat gtctcgtctg      7740
     accctatcgc tgctactgag cttgccagct tgttttttga tacctgggtt gatgggagta      7800
     agctcgcaga gtgcgtcggg gacacaatat cccaaaatgc agtggcggcg tcgtcgtggc      7860
     tcgatagcag gtcttctatg gctaagcagt cgttgtgggc tcgtttgcgg tcttttgtct      7920
     acgatctgtc ggcaatgact cgttataagc ttatggttaa gtcagacgcc aaaccgaagt      7980
     tggactccac gcctttacag cagtacatta cagggcaaaa cattgtgtac catgatagag      8040
     ctataactgc gatgttttca cacatcttta cgcaagcggt cgagaggttg aaatatgtgc      8100
     tgcatacgaa agtcttagca taccacggta tgtcaacaga agacttcagt cgcgaggttg      8160
     gtgatagact gggggatgtg tcgggttatt atgtttacga gctggatatt tcaaaatatg      8220
     acaagtctca gggcgcgtgc atgaaggacg tcgaaaggcg aattttaatc ggtctaggtg      8280
     tgtcggaatc tgttgtagat gctttcttct gtggagagta tgattccgtg gtcacgatgg      8340
     ggaagaacga gctcgttttg tcggtagggg cgcagcggag atctggaggt gcaaatacgt      8400
     ggttagggaa cactttggtt cttatgactt tgttggcgat atctttgaaa ggttcggaac      8460
     cgtctttggt tgttgtttgt ggcgatgact ctttgatttt ttcgaagaac gagctgcaga      8520
     tagatgatag gattcttgaa gagagttttg ggttcgacct caagttaacg tgccagtgtg      8580
     ctccttattt ttgttcgaaa tatttggtga ggacaccgga tcattgttat tttttacctg      8640
     acccgtttaa gttgtttttg aagctaagct tggaaagaga gcccaatgaa gatttgttgt      8700
     ttgaagtgtt tacctcgttt gttgatttga cgcgaggttt tggggatgag agcgcagtac      8760
     agcagcttgt cgcgatggat gcacagaggt atggtcctaa tccatacgca tacgcagcgt      8820
     tttgtttagt gcatatactg gcagcaaatt ttgtgcaatt caagaggtta ttttatgatg      8880
     ctgatggggt gtttgggaag aactcaaaga gtaaggctac tcgatgagct ctatatgtaa      8940
     tggtctcgta taccagggaa tacggctcaa ttctcgacca cccgtaattg gggagaaaag      9000
     atcagggatc gggtgtctac tatcctgaag tagaggtaac ggctatttga gctaggtcgt      9060
     tacaatgtaa tgtagtgtgc agtttttctt agctttagtt catggtttta ggtgttttta      9120
     atttttagta gtgtcgcgtc ttttattctt tcttttcgta gtttattttt gtctatttat      9180
     gttcgtctta gtatagagta gtctagggtg gtagagtaag tttgtggtgt cttcgtctca      9240
     gccgggccgt acgcctcgga ggagttcacc gcacaatcgt gggtactagc gcgttatgtg      9300
     ccgtaaaggc accaggggat gaggcgtacg gcctggctga attgggagat tcaggtaaga      9360
     ttgtatatag gtgtttcgtt gtatagcgtg tacatacgtg ttaaggcgtc ttagtgtgtg      9420
     tatgccgtca tggcattaag ggggtaagca tgcggcttgg ctccttgggg aggttctggt      9480
     aagaatgtta ccatgtattg taatatatat agtgcaagta cgtagtatgg cattataaca      9540
     ccaaaatcgg tagtgttaaa atcagggtta tcccaggttt gcatattaat cgtcaatatt      9600
     taactgtgat atcgccaagt ttgtaatata ctatcagtac agcggtgtgt tttgggtttg      9660
     aataatatac actttcttta tggacttgcg acagttttcc cacgaacttc tatataccgt      9720
     ttctcttttt atactagtag ccttgtgttt tgtcgtatat tttatcgtaa gagctattcg      9780
     gcactgttgt gcgaaaatcg aggataaacc ggtgcgagaa ccaggaaatt catcgtatag      9840
     gtacgtagct caaccgtaga cggaatggaa gtgggacttg atttcggcac tactttcagt      9900
     acggcttgtt tttcggttcc atcggaagat gattccgggt gcgtaacgtt ggtgaattct      9960
     ccgttcgttc caacgcaggt ttttatagga agtgatatga cgtatagcat tgggcacaga     10020
     gcctactccg attttgtagc aggcaaaccc gggggacttt atatcaatcc gaagcgttgg     10080
     gtgggcgtcg acggctacaa ttttcaggct gttaagcgaa agctgaatcc agaatacgaa     10140
     gttaaactga gtaatggtga agttgtttta ggcagcgtgg gtaatgtgaa tgcgccattg     10200
     gtcagagtag ttgatttggt ttttcttttc gtgaagggta tattgctaga aacagaggaa     10260
     gcggtaggca aagcggtatc aggcgttgtc tgtaccgtgc cagcggagta taactcgttc     10320
     aagcgaagtt ttttgggtgt cgctctggaa gggttaggta agccattgag ggctctcata     10380
     aacgaaccaa cgtcagcagc tttgtacgga gctgttaggg gaggtgcgct gaaagagacg     10440
     tacgccgtct tcgacttcgg aggagggact ttggatatat cgttcatatc aaggtttaat     10500
     aacgtcgtga gtgtgctatt ttcgaaggga gacaactttc tgggaggtcg cgacatagac     10560
     agagcgatag tgcagtttct gcgtaaggag aaacgaataa ccggagtgat agatgcgggt     10620
     atattggcag tgatgatagc cgacctaaaa gaaaaaattt gtgttaatgg tggtacacaa     10680
     tatacgcagg tgaagacgtc aaacggctta gaaacgttgt caatgtccgt agacgaattg     10740
     aacgctgtct ctgaaccatt tgtagacaga gcaatcaaaa tcttcgcaga aggtgctaat     10800
     gagcttaaac gtagtccgat tgtctgtgtt ctaaccggcg ggtcggtcgc tttaccgttg     10860
     gtacaacaaa agctaaagac gttaccgtat gttagccgaa cggagtacga ctctaagaca     10920
     tttagattat cggtggccgt aggtgctaag atttatagtg atattctaac ggggcatagc     10980
     gatttgcgac taattgacac ggtttcccaa acgttgtctg acgaattgtc aggtttcacg     11040
     gagatcgtaa tttttcccaa gggtcacccc ataccgtcgg aatatgaaac aagttttcaa     11100
     atatcgggaa gtactatgga gtatggaata atagaaggtg agtctaatcg tacttggttg     11160
     aacgagatcg cttttaaagg tacggattac agaccatcta acgaaaggaa gagtgataag     11220
     gttaaatacg agatatcagt agacggaaaa ttgaaactga gcgtcgacgg tagacaattg     11280
     aaaaataccc gattaccagc gccggtgagc gcgtcggcgc acgcgtatcg ctacgtatcg     11340
     agtatgaaga agtatttaat tcaactcgag aataattacg tcgatatgtt ctctgagtta     11400
     catggcgata aaatatcgat cgacgacgtc tatgatgcta caggtgcgta cttcgacaag     11460
     aatatactag taaacttttt aagattgtcg aaatgaatcc attggtctgg ttgaccggag     11520
     ctaccgatta tatgtcggtg cttaggccac tttacgagga tttcaaagct gggggcggtc     11580
     ggcgcgtagt gaattatatc gaagttatag atcgcggtga taagccgcat ataattcggg     11640
     ggttgtctcc ctcagatgag aacttcgaat tgatagcatg ggcagtagcc gttggtgctt     11700
     tcgagaactc aatttatgcg ttcgatacga tcctttctac gatgcgtaat tggaaggcgt     11760
     acgcgacccc tgggtcgtta caattagaca cgccgataac aaagaaaaag aataagcact     11820
     tcacgttgac agctgaacag caggcagcgg taagaacgtc accgaatagt acgaatgaag     11880
     cttttgcgca attgttcttg aatttaggcg ttttcttgaa acgcgtacca aaggacgaag     11940
     atatatcagg acgaggcaat atcccacttg tgcgtgtgtc acaagcggag tcgacagata     12000
     gaatagaaca gggaaggtac agtcctcttt tggcaaagtt tgtacgtaag tatactcacg     12060
     ttgttgacgc gtacgattcc gttgtcactc gccaaaagat tgaggtgtgt agttattaca     12120
     ttcctgagtt aataggattg ctttttcgag cacgcgatgg cgctcaggca tataaaacgg     12180
     tttcgttata tttggcctcc tttttgcagt ctaaggatcc tggaaagtac gatcccgaaa     12240
     cgagagttat atggtttgcg cggacgttaa cgatggctcg cgaactatta gagatctatt     12300
     acaatattcg cgttgaagtc agcgaaaggg aaattattcg taacttcagg attcaacctg     12360
     gagacgaatt ccatcagctg tcgacaatgt tgttggggtt ggtagcagac acgtcggcta     12420
     agttgacggg gataaccgat gttgatgttg tactaaggag agtcctgggt ctgtgtttaa     12480
     aagctatggt tgatgaaaat caacatatca gtaacatcaa ctataccggt gcgctttatc     12540
     taatgctagt ttatttctcg atagttggaa ctaattactt gaggaagaaa gatacaccta     12600
     acgaaatggt tttcagaatt aacggtgttg tgcggaaagt aaactttact catttaaaga     12660
     ggcttgtggc tgaagcgtcg gttgacggga ggaattatcc gagagaaatt tcgagtgttt     12720
     ttgctgggat tactttgcaa ctacgccagc taggtaatat agacacggta gtatggccgg     12780
     atgtggtctt aagtaatccg agtcttgggt tcgataccgt tttacatagc gggtacatta     12840
     gtttgaaccc tgctatgtac aaggatatcg agattatcaa aagaaagata cggaattcaa     12900
     ctcattgggt gggtggatat aaatatggaa aaagattttg atattatcat ctaagtgata     12960
     aggatactca taaagcgaca aatttcgtgt tttaacagta ttttgattcg gtaatacatc     13020
     gtgcgtttta cggtatcgat ttaagatggc tagcgttata tctcaaaatg atgatgacta     13080
     taacgtcgtc cggggaggaa atatcgttgt gccgcgcacg cctactttaa atggtttcgg     13140
     tacatcggcc ttcactatac ctgcaggtga ggcgactgcg tacgttttga aaacacagta     13200
     ctcgaagcca gaagctggta ctcccgaggc aaatcaccct gtgattggtg ttctgccaga     13260
     cgagtatgtg ttcgtgaaag gtccgggagg ttatactctc cgtccttctc gaccgtctac     13320
     gggtcagcgt tcgggggatt ctgaagtggc taagcgagaa atgggcgata agttaaagag     13380
     aactttcaat atagcagaaa tctttactaa tcccgagatg aatattatct ttgaaccacc     13440
     aaaggatatg gaagtttcgg ttgtggtgcc gaccggaccc ggcttggtga ctccggcagt     13500
     ggcaacagct atatctacgg aattaaaaaa tttatgtgct gaagtgatgg gtaacactga     13560
     tcagaaaagt ctcacagatt ttttcttggc aatgttgcaa ttaatgttga cgtttagcac     13620
     atcaccagac actgagagca aagaagagta cttcgtgaat ttgtacagta acggcgagcg     13680
     aaaattaact tacgagaagg ttaagggcgc cgttgtcaaa ggagctgaag gctcgacgtt     13740
     cgaaaatcct atgcgtcagt atgccaggtt gttctcagca acggctgtac atttaatatt     13800
     gaatggaaag ttaaggccga acgagaaagt agctatgcaa cacggcgtac ctaagaggtt     13860
     tcttccgtat acttttgatt tctgcagacc atcttattct caatttagca atgacgccat     13920
     tagagcatgg cagttggcgg cagaatcggc gttcgggcgg aagagtaatg taacaagctc     13980
     agtactaaga aacaccagtg agctcaaggt gtagatttag aaagctgtga ttaataatga     14040
     caacggcgtt agtggctcct atagttgggg ataaaggcga gtatttgtct ttactgagaa     14100
     acttgccgaa tgataattcc caagaatctt ggctccctaa cgcgggtcgg atgaatgatg     14160
     atttctttaa aataggagag gcgtataagt tacaaaatct ttcgataggg tacacgatta     14220
     atgtatctgt ttcgattaaa tcgatcccaa aagaaacgat gggcgtgctt agagtaataa     14280
     tagaaccacg ggacggtgat atgcttatac acactctaag attctatcgt gacaagaact     14340
     taattgaaca ggtctacacg caaagaaaag gtggcgtcgg tagtgttctc aaaagtagac     14400
     cggtggattc gtcaatcgta aattttcata atgaaagaaa agaaactatg acctcaatgg     14460
     ttaataagca gaataggtta atggaagtct ttacgaatgg ctctagttta ggatcgagcc     14520
     aaatcaaagt cccagtcgac agtttgatca ctgttatagt ttcgttagag tcaaagaaag     14580
     tttcatgcga tggactctcc gaattggtac cgaacgacgt gggctcgatt gtgaaattcg     14640
     acggaacggt cgattacaag gaaataaatc gcgaacggaa gtatatcttt ccgtataaga     14700
     cgtttaggga ttcgaaatac cgggagatct taaccgaagc gattgctgca cgaaacgaaa     14760
     cagcggcggc tcagcctgaa acagagacag ttccggtgca tccgatcgcg aacgtgacct     14820
     atgtaactcc accagagggt gtatcgataa caaatgcagc tatccctgaa actaaaccca     14880
     ttgacgaagt agcgacgtta cttactgcga ttgagaacgt caccgattta cctataggta     14940
     ggtcgaagtt gaatgtttca gaattggatg ttatcgagtt gacgggatat tacaatccca     15000
     gtgttatgga agcagacgat gttcgctctg ttaacgcgaa attactggat tattattcgc     15060
     ggttgtacgg aagatcagaa agttcgaacc gtgcgctggc gataggtatg atccaaggag     15120
     cgctcacgtg gagtacaagt gcaaatctca aggacggtga gatacggaaa gtggatgtat     15180
     cactgaacgg aagaaaattt acagtggact ttaacgaact ccggcatatt atacagtcgt     15240
     cggtaccgca gtcaaaatac gagaaccccg tcagacagta tatgcgttgg ttttctacaa     15300
     cgactataag tctgataaag tctggcgtgg tagtaccaaa ctatcatgta atggctcgac     15360
     acggtgtgac tagccaattt ataccatatg gttttgacta ttgtatatta ttaccttcgt     15420
     ataacaggag agacgataaa gtagcggccg cgttggctag agctcaggct atagcgttag     15480
     cgaacaaaag acgggcagga aagacactat ataacttttc tgaattagaa aaatcataaa     15540
     tggagatcga cgaggcggtc gattcgagaa acgcgattcg tatctatgac gtcaagcgcg     15600
     cgttgctctc tctgtccaag tctatggttt tacgtagcag ggtcaggttc gttagaagag     15660
     ctggtttccg gagtttatat caagtgtgga tcggtagagg taaaaacgta ttagagttgg     15720
     gtgtagactt cgggggcact catggtgcta aactacgacc ctttgtttac tacggtggtt     15780
     catattatta taatgagcaa atggttccaa ctgatatatc ggaagatgta ttggattatg     15840
     tgaacgttga gatcagtttg actctctcaa agaaaagagt cgtaacggct gctttaataa     15900
     acggaatcga cgtgccgtgc ctaggttacg ccttgaaaaa cggaacgctg cgattcggac     15960
     acgaaattat ttttaaagtt gatgacgaca gtttacatta tctattagat aaatcgacta     16020
     ggaataaacg aatcgaaggg ttgtatactg aattagtcgt taaggtcgat ggcggtgacg     16080
     ttttattggg agacgttgat atggcaggta cgccactaga cagagcgtac tcaaacaaaa     16140
     cggggatcga gccttctata cacgttgcga acgtgggaga ctggaaaagc ttgtttgttt     16200
     tatcagcttc tgacacgagg acaggcgtcg aacaacccta taccgaaaat gcaatgcttt     16260
     actatgaagt agtcgataga aatttagaag acgtagcgaa aacgtgcggc tattttgaaa     16320
     taccgaccgc tactgttgct ggattaacta tggtagactt cgaggggatg gaaaaggtaa     16380
     cgctttccgt cagtggtgaa agtacttatc ttagcaaaga cgttgccaag agaataacac     16440
     tggcgttgtt gagactaccg ttgacggtcg acgacgggag tgtgttgact gataaaagac     16500
     tgctattggt attaataatc cagggagcag tcacttatgg aacgcgagcg gatttcgtag     16560
     agaaaggccc gtctaacgtt tgtgtcagtt acaagggaaa gagtgtgcgg ttagatttta     16620
     tgtatgtcag aagtgtgata atagcaaatt gtgacagcat atatattact aatcctatac     16680
     gcaagtacat gcgttggtgg tctacagcga ctatacagct cattaagttg ggtatcgtta     16740
     aaccaaatcc aatagtggcg gcgaagagag gattatcgag taataacata tggttatcat     16800
     tcgactatat actactggac gcgaggttta acgatagaaa agaaaagatt ttgttgcggt     16860
     gatagttggt tcactgagtg aaataactct atgtaaataa tatcactaaa gctttataaa     16920
     gtaattagac tataaattta aaaatatgga atttgctcca gtgtgttata tttgtactaa     16980
     gaatgaagaa ggaagtgata agaaattaac gcttttgtct cgtgaggatc tcatcgattt     17040
     taacagtgac gagaaccaag atgagctctc tttggagaaa aagcccttcg taaaatctta     17100
     cgagaattat ctgcgagatt gggctgagag accgttttat ttctctaatg ctatgtcggt     17160
     tatggatctg gctatgcaca cggggaatgc atcgatagac tcagattctg gtttaagcaa     17220
     gtcgggtgag aagaggcgta ccgctgtaga agttcaaagt ccttggaaaa ctggtatccc     17280
     gttcgatagc gtcgataacg gcgtcttggt ggcagtaggg tacgaaggtt taaatgcgga     17340
     taagaaaaag gcccgttccg tggctctatt tatgcagaac gagggatctt atataatgtt     17400
     gataggcggc gaagtgtatg cgtcgtcgtc atacattaaa gagtgtatgc gctgtcaacg     17460
     cttggcatca acgatgagcg gggacgtgtt cgtagatgca ttgagactgg tttgagggta     17520
     atcaaagtta cccactgttt gttatacata ttgctacgat ggctttgatt acgcctaagt     17580
     atgttggtaa cttctctgag ttttgcgtac acatcatcga aatttcgaac gcggatagcg     17640
     ttcacttact ctatgaagcg ttaaaattga attctcgatg gttagagtta tcgttctcga     17700
     catttgtgga ggagacgcag aaggaattag agcagacgcc aaacggttgg cagttgaggt     17760
     tcgctcgtga gaacacggtg gccgaaattg acaccaagtt tatgcgtaag atgtatggaa     17820
     tgtacctgcg cgaggcagac acactcaaag acatacgcaa ggcgttacat ttgattttag     17880
     ttgacacgaa gttgaccgat gctagcgtaa acggtctagc tcggggtaga gggaagaaaa     17940
     atttttctcg ttcgttgctg aaaggaatga tgagagcgat acgggaactt caatcgagtt     18000
     tagacaaaat tggggtgaaa tcgggggctg aggtcaagct aacagctata gcgatatcgc     18060
     tgttggccga agacagtcac agtagcccga aagatgttat tttgagggtt aataggcttt     18120
     catcctatta tttgctcaaa gataatgtca cgtttcactt tttcggggct atagccagca     18180
     atttggtgta agaagagtca gatgtgattc gttttcgtat cttcaccaat gagcaagaag     18240
     aggtgaattg gttggtatca tttccatgat ccaacatcga tgtccgtcag tgcgagacat     18300
     ttatattgac aattggcgtt cagagactaa caacggtgaa aaatgcggtt atggcacgtt     18360
     caccatcaca aaaggcgcca atacgcttta atcgacgcgt atattatgat tattattgtt     18420
     ctaataatgt tgttagtgct tactttttat ttaattaaga agaggcgatt aagttaaatt     18480
     aaaataaaat aaaataaaat aaataaatgg ctcctaacgc taattaccgg ggagtgaaat     18540
     atcgaaatgt tacttagtaa taagataatc tgagggtcta ggttctcaga ttatataagt     18600
     cctattaggg tgagatatat aaataaacgg aaacgcgcgg acatcgatgt tggatcatg      18659
//