ID   GU983872; SV 1; circular; genomic DNA; STD; VRL; 983 BP.
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AC   GU983872;
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DT   27-JUN-2010 (Rel. 105, Created)
DT   27-JUN-2010 (Rel. 105, Last updated, Version 1)
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DE   Faba bean necrotic stunt virus isolate Holetta-1997 segment DNA-U2,
DE   complete sequence.
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KW   .
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OS   Faba bean necrotic stunt virus
OC   Viruses; Nanoviridae; Nanovirus.
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RN   [1]
RP   1-983
RX   DOI; 10.1128/JVI.00607-10.
RX   PUBMED; 20592071.
RA   Grigoras I., Timchenko T., Grande-Perez A., Katul L., Vetten H.-J.,
RA   Gronenborn B.;
RT   "High variability and rapid evolution of a nanovirus";
RL   J. Virol. 84(18):9105-9117(2010).
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RN   [2]
RP   1-983
RA   Grigoras I., Gronenborn B.;
RT   ;
RL   Submitted (08-MAR-2010) to the INSDC.
RL   Institut des Sciences du Vegetal, Centre National de la Recherche
RL   Scientifique, Gif sur Yvette 91198, France
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DR   MD5; 28320348bc09e2360125391e0086eda6.
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CC   GenBank Accession Numbers GU983866-GU983873 represent the complete
CC   genome of Faba bean necrotic stunt virus isolate Holetta-1997.
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FH   Key             Location/Qualifiers
FH
FT   source          1..983
FT                   /organism="Faba bean necrotic stunt virus"
FT                   /segment="DNA-U2"
FT                   /host="Vicia faba"
FT                   /strain="Ethiopia:Holetta"
FT                   /isolate="Holetta-1997"
FT                   /mol_type="genomic DNA"
FT                   /country="Ethiopia:Holetta"
FT                   /isolation_source="plant tissue"
FT                   /collected_by="K.M. Makkouk"
FT                   /collection_date="Oct-1997"
FT                   /identified_by="L. Katul and H.-J. Vetten"
FT                   /note="acronym: FBNSV-[ET:Hol] DNA-U2"
FT                   /db_xref="taxon:283824"
FT   stem_loop       join(964..983,1..13)
FT                   /note="part of replication origin"
FT   misc_feature    join(977..983,1..2)
FT                   /note="conserved nonanucleotide at replication origin"
FT   misc_feature    1
FT                   /note="first base of viral (+) strand"
FT   regulatory      259..265
FT                   /regulatory_class="TATA_box"
FT   CDS             330..695
FT                   /codon_start=1
FT                   /product="U2 protein"
FT                   /db_xref="UniProtKB/TrEMBL:C7DLN8"
FT                   /protein_id="ADJ00312.1"
FT                   /translation="MDAGFEYNLSMREIMLLKEEQDRFWSSYHDFLRAHEDVLGEFCRL
FT                   HGRRVLAYPRLPSYAPTRLVLKSRTVYDIRVDECKTCIAECKNSSCNNDGSLEGLCDLY
FT                   DYGNYRYQVRYSNTSCN"
FT   regulatory      705..710
FT                   /regulatory_class="polyA_signal_sequence"
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SQ   Sequence 983 BP; 290 A; 172 C; 206 G; 315 T; 0 other;
     acccccgccc caggttcagc ggagtcatca cgtgagacgc acatgctatt aaatgaatta        60
     taattaaata agtgggggat ggtccccact ccgcactaac ttcaaataca ctgaatatat       120
     gctttgcttc gtcacgaagc aaaggtaaag aataaaaact ggtccccaca acgctttata       180
     tgcaattcag tggattcaaa cgagtggttg atattcatta cgttaaaata gcttacagag       240
     gaagcttggg ttatgtacta taaattcaat atatctgaat tgtttattca gatcgtcttc       300
     ttccatcccc attttctgtt ttggttctta tggatgccgg ctttgagtat aatctctcta       360
     tgagagagat tatgttattg aaagaagaac aagaccgctt ttggagctca tatcacgatt       420
     ttcttcgtgc ccacgaagat gttcttggtg aattctgcag actccatgga agaagagttc       480
     tagcgtatcc aaggttacct agctatgctc ctactcgttt ggtccttaaa tctagaactg       540
     tatatgatat tagagtagat gaatgcaaaa cttgtattgc agaatgtaaa aacagttctt       600
     gtaataacga tggcagtcta gaaggtttat gtgatttata tgactatggt aattacagat       660
     atcaagttcg ttattctaat acttcatgta attaagatat atgtaatata tttgaagcat       720
     aattaatgca gtgatgaaat taatatgttg ttgttgtctt gtgtaattgt tgtgttgttg       780
     aagaaccaga gagtcccaag gtagaagaca actactatac acgtgttatc acgggattgg       840
     ttattattat ttgggtttac tccgcgtagc ggtatgcttc agcccaatag gcccaattgc       900
     tctcaaggcc caatagaatg accaatagga atgaagcatg acgtcatttg atcccgtgct       960
     gagctggggc ggggcttagt att                                               983
//