ID   GQ150784; SV 1; circular; genomic DNA; STD; VRL; 984 BP.
XX
AC   GQ150784;
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DT   10-AUG-2009 (Rel. 101, Created)
DT   27-SEP-2009 (Rel. 102, Last updated, Version 2)
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DE   Faba bean necrotic stunt virus isolate JKI-2000 segment DNA-U2, complete
DE   sequence.
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KW   .
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OS   Faba bean necrotic stunt virus
OC   Viruses; Nanoviridae; Nanovirus.
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RN   [1]
RP   1-984
RX   DOI; 10.1128/JVI.01212-09.
RX   PUBMED; 19656882.
RA   Grigoras I., Timchenko T., Katul L., Grande-Perez A., Vetten H.J.,
RA   Gronenborn B.;
RT   "Reconstitution of authentic nanovirus from multiple cloned DNAs";
RL   J. Virol. 83(20):10778-10787(2009).
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RN   [2]
RP   1-984
RA   Grigoras I., Gronenborn B.;
RT   ;
RL   Submitted (13-MAY-2009) to the INSDC.
RL   Institut des Sciences du Vegetal, Centre National de la Recherche
RL   Scientifique, Gif sur Yvette 91198, France
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DR   MD5; c0b10fbc966906c8a55b2fb91261adb5.
DR   EuropePMC; PMC5533907; 28566384.
DR   EuropePMC; PMC6414197; 30857590.
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FH   Key             Location/Qualifiers
FH
FT   source          1..984
FT                   /organism="Faba bean necrotic stunt virus"
FT                   /segment="DNA-U2"
FT                   /host="Vicia faba"
FT                   /strain="Ethiopia:Holetta"
FT                   /isolate="JKI-2000"
FT                   /mol_type="genomic DNA"
FT                   /country="Ethiopia:Holetta"
FT                   /isolation_source="plant tissue"
FT                   /collected_by="K.M. Makkouk"
FT                   /collection_date="Oct-1997"
FT                   /identified_by="L. Katul and H.-J. Vetten"
FT                   /note="acronym: FBNSV-[ET:Hol] DNA-U2"
FT                   /db_xref="taxon:283824"
FT   stem_loop       join(965..984,1..13)
FT                   /note="part of replication origin"
FT   misc_feature    join(978..984,1..2)
FT                   /note="conserved nonanucleotide at replication origin"
FT   misc_feature    1
FT                   /note="first base of viral (+) strand"
FT   regulatory      259..265
FT                   /regulatory_class="TATA_box"
FT   CDS             330..695
FT                   /codon_start=1
FT                   /product="U2 protein"
FT                   /db_xref="UniProtKB/TrEMBL:C7DLN8"
FT                   /protein_id="ACU00027.1"
FT                   /translation="MDAGFEYNLSMREIMLLKEEQDRFWSSYHDFLRAHEDVLGEFCRL
FT                   HGRRVLAYPRLPSYAPTRLVLKSRTVYDIRVDECKTCIAECKNSSCNNDGSLEGLCDLY
FT                   DYGNYRYQVRYSNTSCN"
FT   regulatory      705..710
FT                   /regulatory_class="polyA_signal_sequence"
XX
SQ   Sequence 984 BP; 288 A; 174 C; 207 G; 315 T; 0 other;
     acccccgccc caggttcagc ggagtcatca cgtgagacgc acatgctatt aaatgaatta        60
     taattaaata agtgggggat ggtccccact ccgcactaac ttcaaataca ctgaatatat       120
     gctttgcttc gtcacgaagc aaaggtaaag aataaaaact ggtccccaca acgctttata       180
     tgcaattcag tggattcaaa cgagtggttg atattcatta cgttaaaata gcttacagag       240
     gaagcttggg ttatgtacta taaattcaat atatctgaat tgtttattca gatcgtcttc       300
     ttccatcccc attttctgtt ttggttctta tggatgccgg ctttgagtat aatctctcta       360
     tgagagagat tatgttattg aaagaagaac aagaccgctt ttggagctca tatcacgatt       420
     ttcttcgtgc ccacgaagat gttcttggtg aattctgcag actccatgga agaagagttc       480
     tagcgtatcc aaggttacct agctatgctc ctactcgttt ggtccttaaa tctagaactg       540
     tatatgatat tagagtagat gaatgcaaaa cttgtattgc agaatgtaaa aacagttctt       600
     gtaataacga tggcagtcta gaaggtttat gtgatttata tgactatggt aattacagat       660
     atcaagttcg ttattctaat acttcatgta attaagatat atgtaatata tttgaagcat       720
     aattaatgca gtgatgaaat taatatgttg ttgttgtctt gtgtaattgt tgtgttgttg       780
     aagaaccaga gagtcccaag gtagaagaca actactatac acgtgttatc acgggattgg       840
     ttattattgt tttgggttta ctccgcgtag cggtatgctt cagcccaaca ggcccaattg       900
     ctctcaaggc ccaatagact gaccaatagg aatgaagcat gacgtcattt gatcccgtgc       960
     tgagctgggg cggggcttag tatt                                              984
//