ID   FJ807730; SV 1; linear; viral cRNA; STD; VRL; 1305 BP.
XX
AC   FJ807730;
XX
DT   09-JUN-2009 (Rel. 101, Created)
DT   07-SEP-2010 (Rel. 106, Last updated, Version 2)
XX
DE   Freesia sneak virus isolate Virginia coat protein (CP) gene, complete cds.
XX
KW   .
XX
OS   Ophiovirus freesiae
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC   Milneviricetes; Serpentovirales; Aspiviridae; Ophiovirus.
XX
RN   [1]
RP   1-1305
RX   AGRICOLA; IND44295012.
RX   DOI; 10.1094/PDIS-93-9-0965B.
RA   Vaira A.M., Hansen M.A., Murphy C., Reinsel M.D., Hammond J.;
RT   "First Report of Freesia sneak virus in Freesia sp. in Virginia";
RL   Plant Dis. 93(9):965-965(2009).
XX
RN   [2]
RP   1-1305
RA   Vaira A.M., Hammond J., Hansen M.A.;
RT   ;
RL   Submitted (06-MAR-2009) to the INSDC.
RL   National Arboretum, USDA-ARS, Floral and Nursery Plants Research Unit,
RL   10300 Baltimore Avenue, Beltsville, MD 20894, USA
XX
DR   MD5; 50c53d3e0ca1085bffbf36153679bab0.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1305
FT                   /organism="Ophiovirus freesiae"
FT                   /segment="RNA3"
FT                   /host="Freesia refracta hyb."
FT                   /isolate="Virginia"
FT                   /mol_type="viral cRNA"
FT                   /country="USA"
FT                   /collection_date="Mar-2008"
FT                   /clone="F2"
FT                   /PCR_primers="fwd_seq: gactcgagaaatgtctggaaaatactctgttc,
FT                   rev_seq: ccaggatccttagatagtgaatccataagctg"
FT                   /db_xref="taxon:2170046"
FT   gene            1..1305
FT                   /gene="CP"
FT   CDS             1..1305
FT                   /codon_start=1
FT                   /gene="CP"
FT                   /product="coat protein"
FT                   /db_xref="GOA:C5I9W7"
FT                   /db_xref="InterPro:IPR021310"
FT                   /db_xref="UniProtKB/TrEMBL:C5I9W7"
FT                   /protein_id="ACR56715.1"
FT                   /translation="MSGKYSVQDVVKVMRASIYSPEQKELLMSLKLINDKEQVNEVNRT
FT                   LLYQASKENPDNIVHLKTDDTLMFIASETVKQPQAPITAPEETLAFKPEIIKDIIEAKT
FT                   LALDQDKIQAVLDQYVKDLPKSGDTYKSGDVIVKEFKNVKLNISSLLAAGTKVLDAILY
FT                   MTYKDSADHSFIFSKETLSPESVVKKEIASRIDIGNKAIKSSFCLVYNQGGLPSKSSSN
FT                   KTLSKFIKETVFRDSKMDSDTLCDFLSSADPSFFPASVFLNIPLDHVHTEVASRCKMAI
FT                   AGNKAIRYAKFAAKFEKDQVNEADYKSNENIKELVVKEEKLKTAHAIVEFLCSLASNFE
FT                   AQKKMHPLSPGRSSRKNFTLQLTCAIMFSRSKQGRMDMRSAITSQKIESFKRDENLYGS
FT                   VDSLGHVSFPIFENGEADFSELSVPAIRAAYGFTI"
FT   primer_bind     1..22
FT   variation       238
FT                   /gene="CP"
FT                   /replace="a"
FT                   /note="results in Ala to Thr substitution"
FT   variation       495
FT                   /gene="CP"
FT                   /replace="t"
FT   variation       663
FT                   /gene="CP"
FT                   /replace="g"
FT   variation       927
FT                   /gene="CP"
FT                   /replace="a"
FT                   /note="results in Asn to Lys substitution"
FT   primer_bind     complement(1283..1305)
XX
SQ   Sequence 1305 BP; 431 A; 237 C; 248 G; 389 T; 0 other;
     atgtctggaa aatactctgt tcaagatgtc gtcaaggtta tgagggccag catctacagt        60
     cctgaacaaa aggaactttt gatgagcctt aaactgatta atgacaaaga gcaagttaat       120
     gaagtcaaca gaactttgct ttatcaagct tcaaaagaaa accctgataa cattgttcac       180
     ttgaagacag atgacacatt gatgttcatc gcaagtgaaa cagttaagca acctcaggcc       240
     cctattactg cacctgaaga aactttggca ttcaaacctg aaatcataaa ggacatcatc       300
     gaagcgaaaa ccctggcttt agatcaagac aagattcaag cagttttaga tcagtatgtc       360
     aaagatttac ctaaaagtgg agatacttac aagtctggtg atgtaatcgt gaaagaattt       420
     aaaaatgtaa aattaaacat tagcagtctc cttgctgctg ggactaaagt tttggatgcg       480
     attctctaca tgacctataa agattcagcg gatcacagtt tcatcttctc taaagagact       540
     ttgagtcctg aatctgtggt taagaaagaa atcgctagca gaattgatat tggtaacaag       600
     gctataaagt catcattttg tttagtctac aatcaaggcg gtttaccatc gaaaagttct       660
     tcaaataaga cattgagtaa atttatcaaa gaaacggttt ttcgtgacag caaaatggac       720
     agtgacactc tttgtgactt tttgtcgtct gcagaccctt cctttttccc agctagtgtt       780
     tttctcaaca tacctcttga tcatgttcac actgaggttg cttccaggtg taagatggca       840
     atagcaggta ataaagcaat caggtatgct aaatttgctg ccaaatttga aaaagaccag       900
     gtgaatgaag ctgattataa atcaaatgaa aatattaaag aattagtcgt aaaagaagaa       960
     aagttaaaga ctgctcatgc tatcgttgag ttcctttgtt ctctagcttc aaatttcgaa      1020
     gctcaaaaga aaatgcatcc actttctcct ggtaggtctt ctagaaagaa cttcactctt      1080
     cagctgacgt gtgcgataat gttttctcgt tctaaacaag gcagaatgga tatgagaagc      1140
     gcaatcacat ctcaaaagat agaatcattt aaaagagacg aaaacctata tggttctgtt      1200
     gattcacttg gtcatgtttc ctttcccatt tttgaaaatg gtgaagctga tttttctgag      1260
     ctttcagttc cggctattcg agcagcttat ggattcacta tctaa                      1305
//