ID   AB060963; SV 1; linear; genomic RNA; STD; VRL; 1724 BP.
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AC   AB060963;
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DT   30-OCT-2001 (Rel. 69, Created)
DT   30-JAN-2009 (Rel. 99, Last updated, Version 4)
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DE   Apple chlorotic leaf spot virus genomic RNA, 5750-7512 nt. position,
DE   isolate: MO31.
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KW   .
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OS   Apple chlorotic leaf spot virus
OC   Viruses; Riboviria; Tymovirales; Betaflexiviridae; Trivirinae; Trichovirus.
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RN   [1]
RP   1-1724
RA   Nakahara K.;
RT   ;
RL   Submitted (29-APR-2001) to the INSDC.
RL   Contact:Kenji Nakahara Akita Prefectural University, Biotechnology
RL   Institute; Minami 2-2, Ogata, Akita 010-0444, Japan
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RN   [2]
RA   Nakahara K., Yoshida K., Suzaki K., Ito T., Yoshikawa N.;
RT   "Nested PCR Assay Developed for Detection of Apple Chlorotic Leaf Spot
RT   Virus Heterogeneous in Apple";
RL   Unpublished.
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DR   MD5; 41e4f71e733ca02ba6220efb077c7247.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1724
FT                   /organism="Apple chlorotic leaf spot virus"
FT                   /map="5750-7512 nt. position"
FT                   /isolate="MO31"
FT                   /mol_type="genomic RNA"
FT                   /isolation_source="Apple"
FT                   /db_xref="taxon:12175"
FT   misc_feature    1..1724
FT                   /note="This cDNA is amplified with forward primer:
FT                   GAAGATCGCAGAAGGGGATATTC and reverse primer:
FT                   GTCTACAGGCTATTTATTATAAG, corresponding to 5750..7512 nt.
FT                   position of genome sequence reported in Acc#:D14996."
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SQ   Sequence 1724 BP; 537 A; 299 C; 421 G; 461 T; 6 other;
     gtgagtcctt ttaagagggc ctctgacttg atgattcatt ggaatgaatt tgttttcaaa        60
     gtcatgccgg aagacattgc gggggacggt ttcagattgg cgtccatacc tgtgatacct       120
     tcgtcagaag tccaggcggt tctgaggaag agagaaagca caaattatgt gcattgggga       180
     gctttgtcaa tatcaataga tgctttattc aggaaaaatg caggggtttc cggttggtgc       240
     tatgtatatg acaacaggtg ggaaacattt gagcaggcaa tgctmcagaa atttcgtttc       300
     aacttggata gtggatcagc cacattggtg acctccccaa actttcctgt ctcgttggac       360
     gatccaggcc tctcgaattc aatcagcgtg gcggtgatgt ttgagaatct gaacttcaaa       420
     ttggaaagtt atccaattag tgtgagagta ggcaacatgt gcaggttctt tgacagcttc       480
     ttgagcagtg ttaagaacaa agttgattct aattttctac ttgaagccgc aaatgcagac       540
     cctctgggag caggagcttt tgggtttgaa caggatgatc aagtgagtga attatttcat       600
     tacattcaaa ctgttccaac ccaagccatc aaatcaaggg aacatgaaat tcccaaaggt       660
     ttatttggaa tgatggggaa gaagaaagtt aaatcctttg aatttgcttc aggatcaaga       720
     agtatggaaa ggagaaggcc acagagaggg agaccattgg agaggtcaac cagccaaaga       780
     gttatccctg gatttaggtc ccaggacgag aaaattgaac atcaaggttt gtcaactgac       840
     agcgattttg aaaattttct caagaacaaa ggaaaaggaa aagctggaac caaatccatt       900
     gcttcagagg ggtcatccat tgataacatt tctccaagag aatttcagtt tgctcgacaa       960
     gatcaggcga aggagaatgg cagcagttct gaatttacag ctaaaagtgg acgcagatct      1020
     gaaagcgttc ctggccgcag aaggcagacc ccttcatgga aagacagggg caatcctgga      1080
     acagatactg gagtccatct tcgcgaacat agcgatwcag ggcacgtcag agcagacaga      1140
     attcctggat ctartggtgg aggtgaagtc matggaggat cagaaggtaa tcgggtctta      1200
     caacctgaag gaggtggtca acatgatcaa agctttcaag actacctctt cggatccgaa      1260
     catcagcaac atgactttcc gtcaggtgtg tgaggctttc gcaccggagg cgagaaacgg      1320
     gttggtaaaa ttgaagtata aaggggtttt tactaacctc tttacaacca tgccggaagt      1380
     aggaagtaag tacccggagc tgatgtttga ttttaataag ggccttaaca tgtttatcat      1440
     gaayaaggct caacaaaaag tcataactaa tatgaatcgg cgtcttttac aaactgaatt      1500
     tgcaaaaagt gagaatgagg cgaagctctc atctgttaca actgatcttt gcatttagtc      1560
     tgtttaagaa gtttggttta ataaacaaaa taaatagatr gtgtgttgcg tgtttaaata      1620
     tatttgcatt aaatatgttt gcattaaata acaaactctt gaacccatga aagagtataa      1680
     agagtcatgg tatttgattg gagtgtttag acttataata aata                       1724
//