Microarray


Microarray
ArrayExpress definition: A DNA microarray consists of an arrayed series of thousands of microscopic spots of DNA oligonucleotides, called features, each containing picomoles of a specific DNA sequence, known a probe (or reporter). This can be a short section of a gene or other DNA element that is used to hybridise a cDNA or cRNA sample (called target) under high-stringency conditions. Probe-target hybridisation is usually detected and quantified by detection of fluorophore-, silver, or chemiluminescence-labeled targets to determine the relative abundance of nucleic acid sequences in the target. DNA microarrays can be used to measure changes in expression levels, to detect single-nucleotide polymorphisms (SNPs), to detect genomic gains and losses, etc.