One- and two-channel experiments


One- and two-channel experiments
Two-channel or two-colour hybridisation experiments aim to compare the relative transcript abundance in two mRNA or DNA samples (for example a ‘test’ cell state and a ‘reference’ cell state) which are labelled using two different fluorescent dyes (say, a red dye for the test and a green dye for the reference), mixed and then hybridized to the arrayed DNA spots.Affymetrix arrays use instead a one-channel or single-color labeling strategy where experimental mRNA is enzymatically amplified, biotin-labeled for detection, hybridized to the array, and detected through the binding of a fluorescent compound.