RNA-Seq in the Zebrafish gene build

This talk is by John Collins from the Wellcome Trust Sanger Institute. He explains how RNA-Seq data were incorporated into the zebrafish Ensembl gene build. This is an example of creating a gene-build combining RNA-Seq with other data, improving its quality.

He describes how RNA libraries were created from adult and embryonic zebrafish tissues and prepped for Illumina sequencing. He describes RNA-Seq was used to define 3' ends by enriching for polyA tails and trimming, thus improving 3' end prediction.

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Incorporating RNA-seq data into the zebrafish Ensembl genebuild. Collins JE, White S, Searle SM, Stemple DL. Genome Res. 2012 Oct;22(10):2067-78. doi: 10.1101/gr.137901.112. Epub 2012 Jul 12.

This research was supported by the Wellcome Trust Sanger Institute grant number 098051