This talk is by John Collins from the Wellcome Trust Sanger Institute. He explains how RNA-Seq data were incorporated into the zebrafish Ensembl gene build. This is an example of creating a gene-build combining RNA-Seq with other data, improving its quality.
He describes how RNA libraries were created from adult and embryonic zebrafish tissues and prepped for Illumina sequencing. He describes RNA-Seq was used to define 3' ends by enriching for polyA tails and trimming, thus improving 3' end prediction.
This research was supported by the Wellcome Trust Sanger Institute grant number 098051