This exercise was set by Remco Loos and Myrto Kostadima from the EMBL-EBI. The exercise involves using a sample RNA-Seq dataset from zebrafish to perform a number of basic tasks:
- Aligning RNA-Seq data to the genome.
- Transcriptome reconstruction.
- Comparison of gene expression between two different conditions.
The exercise materials are available for your to work through at your leisure.
You will need to install the following programmes and commands on your computer in order to carry out this practical: