Part 3

This last talk by Matthias is 10 minutes long.

In this talk he examines some of the Velvet extensions available:

  • Columbus, which allows you to align reads to a reference sequence.
  • Oases, for transcriptome analysis.
  • Metavelvet, to separate out and assemble a mixed pot of multiple genomes.

He also discusses some issues related to assembly quality, including:

  • Read simulation.
  • Testing of different methods in Assemblathon.
  • Expected and observed coverage.

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