This is a talk by Aylwyn Scally from the Wellcome Trust Sanger Institute. This lecture is 52 minutes long.
- sequence formats and quality.
- alignment of Next-Gen sequences to reference sequences.
- identification and labelling of variants, such as SNPs and structural variations.
- sequence assembly.
For each of these topics, he covers some of the programmes available for carrying out these analyses, the important issues to consider and the kinds of file inputs and outputs involved.