This talk by Remco Loos from the EMBL-EBI looks at ChIP-seq and lasts 35 minutes.
In his talk, Remco explains the principles behind ChIP (Chromatin ImmunoPrecipitation) and how NGS has improved ChIP technology but created challenges in terms of data quantity. He discusses the various stages and issues of ChIP-seq, including:
- Experimental design.
- Mapping software.
- Peak calling, and non-peak based analysis.
- Analysis of enriched areas.
- Viewing ChIP-seq data in genome browsers.