Training

This course is aimed at molecular biologists who wish to gain new skills in the analysis of high-throughput DNA sequencing data. The programme will cover various EBI databases and resources, and third-party software for the analysis, interpretation and storage of genetic sequence data. Using a combination of lectures, hands-on practical sessions and discussions with trainers, you will learn how to manipulate NGS data, analyse the results of different experiments and utilise sequence databases from the EBI..

What will I learn?

By the end of this course you will be able to:

• Navigate the major databases at the EBI needed for NGS
• Understand the differences between the major nucleotide sequence databases
• Browse the ENA database in detail; understand its structure and the type of data it contains.
• Utilize different ways to access sequence data from EBI resources
• Understand the basis of CRAM and the need for data compression
• Use techniques to generate and analyse RNA-Seq data
• Comprehend the different tools involved in whole genome re-sequencing, assembly and variation calling
• Utilize these tools to conduct simple re-sequencing assemblies
• Comprehend the techniques used to generate and analyse ChIP-Seq data Use the MACS and PeakAnalyzer utilities to discover immuno-enriched areas in ChIP-Seq data
• Understand the varied applications of NGS data analysis and how it is being used to answer interesting scientific questions