Training

Is it right for me?

This joint EBI-Wellcome Trust workshop provides hands-on training in the use of public databases for peptide analysis, annotation of subsequent protein lists using these resources, incorporation of information from molecular interaction and pathway databases, and the use of protein 3D-structure data in proteomics experiments. The workshop is aimed at research scientists with a minimum of a degree in a biological discipline, including laboratory and clinical staff as well as specialists in related fields. The practical elements of the course will take raw data from a proteomics experiment and analyse it in order to go from MS spectra, identify peptides and finally to lists of protein identifiers that can be analysed further using a wide range of resources. The aim is to provide attendees with the practical bioinformatics knowledge they need to go back to the lab and process their own data (including large datasets) when collected.

What will I learn?

• Mass Spectroscopy Basics and MS Search Engines
• Sequence databases and their use
• Proteomics data and services integration
• Standardising proteomics data
• Protein 3-D structure data and proteomics

What will it cover?

• PRIDE - The Proteomics Identification Database
• Protein interaction data through IntAct
• Using ontologies to annotate data
• Placing proteins in pathways using Reactome
• Protein sequence data in UniProt and InterPro